Effect of electroacupuncture at “Jiaji”(EX-B 2) points combined with nerve mobilization on motor function and mRNA and protein expression of RhoA in rabbits with sciatic nerve injury

doi:10.13703/j.0255-2930.2019.06.016

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Effect of electroacupuncture at “Jiaji”(EX-B 2) points combined with nerve mobilization on motor function and mRNA and protein expression of RhoA in rabbits with sciatic nerve injury

更新时间：2023-05-10

- Effect of electroacupuncture at “Jiaji”(EX-B 2) points combined with nerve mobilization on motor function and mRNA and protein expression of RhoA in rabbits with sciatic nerve injury
- Chinese Acupuncture and Moxibustion Vol. 39, Issue 6, Pages: 625-631(2019)
- 作者机构：
  
  1. 黑龙江中医药大学附属第二医院康复中心
  2. 黑龙江中医药大学研究生院
- 作者简介：
- 基金信息：
- DOI：10.13703/j.0255-2930.2019.06.016
  CLC：
- Published：2019
- 稿件说明：
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Effect of electroacupuncture at “Jiaji”(EX-B 2) points combined with nerve mobilization on motor function and mRNA and protein expression of RhoA in rabbits with sciatic nerve injury[J]. Chinese Acupuncture and Moxibustion, 2019, 39(6): 625-631.
DOI：

Effect of electroacupuncture at “Jiaji”(EX-B 2) points combined with nerve mobilization on motor function and mRNA and protein expression of RhoA in rabbits with sciatic nerve injury[J]. Chinese Acupuncture and Moxibustion, 2019, 39(6): 625-631. DOI： 10.13703/j.0255-2930.2019.06.016.

摘要

目的:观察"夹脊"电针结合神经松动术对家兔坐骨神经损伤后RhoA蛋白及mRNA表达的影响

为"夹脊"电针结合神经松动术治疗周围神经损伤提供理论依据。方法:将180只新西兰家兔随机分为正常对照组、模型对照组、神经松动术组、夹脊电针组、电针+神经松动术组

每组36只;每组再按术后干预1、2、4周分为3个亚组

每个亚组12只。钳夹法制造坐骨神经损伤模型。正常对照组不做任何干预;模型对照组术后放入笼中安静饲养;神经松动术组进行坐骨神经神经松动术治疗

每次操作时间为1 s

放松5 s

每组10次

每天1组

每周6 d;夹脊电针组取坐骨神经发出的相应脊髓节段L4～L6"夹脊"穴进行电针治疗

每天1次

每次30 min

每周6次;电针+神经松动术组先进行"夹脊"电针治疗后进行神经松动术治疗。采用趾张反射和改良Tarlov评分对损伤侧坐骨神经功能进行评价;取坐骨神经发出的脊髓相应节段(L4～L6)及坐骨神经卡压处组织

实时荧光定量PCR检测方法观察RhoA基因表达的变化;Western Blot法检测RhoA蛋白的表达。结果:①趾张反射和改良Tarlov评分:在1、2、4周时

模型对照组评分低于正常对照组(均P<0.01)

神经松动术组、夹脊电针组、电针+神经松动术组各亚组评分高于模型对照组(均P<0.01)

且电针+神经松动术组各亚组高于神经松动术组和夹脊电针组(均P<0.01)

其中4周组恢复最好;②Rho A的mRNA及蛋白表达:脊髓节段:在1、2、4周时

模型对照组高于正常对照组(均P<0.01)

神经松动术组、夹脊电针组、电针+神经松动术组各亚组低于模型对照组(均P<0.01)

且电针+神经松动术组各亚组低于神经松动术组和夹脊电针组(均P<0.01)

在1周和4周时

神经松动术组低于夹脊电针组(均P<0.01)

在2周时

神经松动术组高于夹脊电针组(P<0.01);坐骨神经:在1、2、4周时

模型对照组均高于正常对照组(均P<0.01)

神经松动术组、夹脊电针组、电针+神经松动术组各亚组低于模型对照组(均P<0.01)

且在2周和4周时

电针+神经松动术组低于神经松动术组和夹脊电针组(均P<0.01);在1周时

神经松动术组低于夹脊电针组和电针+神经松动术组(均P<0.01)

夹脊电针组与电针+神经松动术组比较差异无统计学意义(P>0.05);在2周时

神经松动术组高于夹脊电针组(P<0.01);在4周时

神经松动术组低于夹脊电针组(均P<0.01)。结论:神经松动术和夹脊电针均可以促进损伤的坐骨神经修复

可能与降低RhoA的表达水平有关

且两者结合效果更佳。

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