Effect on p53 and caspase-3 of hippocampal neuron in the rats with cerebral ischemiareperfusion injury treated with electroacupuncture preconditioning at the acupoints on the basis of biao and ben relationship

doi:10.13703/j.0255-2930.2019.09.011

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Effect on p53 and caspase-3 of hippocampal neuron in the rats with cerebral ischemiareperfusion injury treated with electroacupuncture preconditioning at the acupoints on the basis of biao and ben relationship

更新时间：2023-05-10

- Effect on p53 and caspase-3 of hippocampal neuron in the rats with cerebral ischemiareperfusion injury treated with electroacupuncture preconditioning at the acupoints on the basis of biao and ben relationship
- Chinese Acupuncture and Moxibustion Vol. 39, Issue 9, Pages: 957-962(2019)
- 作者机构：
  
  1. 武汉市七医院综合内科
  2. 湖北中医药大学针灸骨伤学院
- 作者简介：
- 基金信息：
- DOI：10.13703/j.0255-2930.2019.09.011
  CLC：
- Published：2019
- 稿件说明：
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Effect on p53 and caspase-3 of hippocampal neuron in the rats with cerebral ischemiareperfusion injury treated with electroacupuncture preconditioning at the acupoints on the basis of biao and ben relationship[J]. Chinese Acupuncture and Moxibustion, 2019, 39(9): 957-962.
DOI：

Effect on p53 and caspase-3 of hippocampal neuron in the rats with cerebral ischemiareperfusion injury treated with electroacupuncture preconditioning at the acupoints on the basis of biao and ben relationship[J]. Chinese Acupuncture and Moxibustion, 2019, 39(9): 957-962. DOI： 10.13703/j.0255-2930.2019.09.011.

摘要

目的:探讨电针预处理对大鼠脑缺血再灌注损伤的保护作用及其抗神经元凋亡相关机制。方法:将60只SPF级3月龄雄性SD大鼠随机分为假手术组、缺血/再灌注组和电针预处理组

每组20只。缺血/再灌注组和电针预处理组采用改良的MCAO线栓法缺血2 h后行再灌注3 h

制备大鼠右侧局灶性脑缺血再灌注损伤模型。假手术组仅分离右侧颈总动脉

不给予其他处理。电针预处理组造模前取"百会""肾俞""三阴交"电针预处理

予疏密波

频率2 Hz/100 Hz

强度1 m A

每15分钟为一刺激单元(通电10 min

留针不通电5 min)

连续重复4次

共计1 h。各组麻醉清醒后进行神经行为学评分;TTC染色法测定脑梗死区百分比

TUNEL法检测海马区神经元凋亡指数(AI)

免疫组织化学法检测p53和caspase-3蛋白表达。结果:与假手术组比较

缺血/再灌注组神经行为学评分、脑梗死区百分比、神经元凋亡指数明显增高(均P<0.01);与缺血/再灌注组比较

电针预处理组神经行为学评分、脑梗死区百分比、神经元凋亡指数明显降低(均P<0.01)。p53为细胞核着色

caspase-3为细胞质着色

两者阳性细胞均呈棕黄色

假手术组大鼠右侧海马CA3区神经元细胞的结构清晰且明显

阳性细胞少;缺血/再灌注组右侧海马CA3区p53和caspase-3阳性表达明显增多(P<0.01);与缺血/再灌注组比较

电针预处理组右侧海马CA3区caspase-3和p53阳性表达明显减少(P<0.01)。结论:电针预处理可能是通过抑制p53和caspase-3的表达抗神经元凋亡

从而改善大鼠脑组织的缺血性损伤。

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