Objective To observe the difference in the efficacy of acupuncture intervention at different time points on the pregnancy rate of in vitro fertilization-frozen embryo transfer（IVF-FET）.Methods Ninety-nine patients with secondary infertility who were going to receive IVF-FET were divided into a transplantation-day acupuncture group

a transplantation cycle acupuncture group

and a non-acupuncture group according to their wishes

with 33 cases in each group.All three groups of patients received conventional IVF-FET treatment.The transplantation-day acupuncture group received acupuncture treatment 1 h before and 30 min after the embryo transfer

and the transplantation cycle acupuncture group received acupuncture treatment 5 weeks before the FET（once a week

5 times） and on the day of transplantation

Zigong（EX-CA 1）

Guanyuan（CV 4）

Taichong（LR 3）

Hegu（LI 4）

etc.were selected in the two groups.The biochemical pregnancy rate and clinical pregnancy rate in the three groups were compared.Results The biochemical pregnancy rate and clinical pregnancy rate in the transplantation cycle acupuncture group were higher than those in the non-acupuncture group（P<0.05）.There was no statistical difference in the biochemical pregnancy rate and clinical pregnancy rate between the transplantation cycle acupuncture group and the transplantation-day acupuncture group

and between the transplantation-day acupuncture group and the non-acupuncture group（P>0.05）.Conclusion Acupuncture during the transplantation cycle can effectively improve the pregnancy rate of secondary infertility patients receiving IVF-FET.