Effects of electroacupuncture on gait and proliferation and differentiation of muscle satellite cell in rats with acute blunt trauma of gastrocnemius muscle

HUANG Yu-ting; CHEN Jia-yan; ZHENG Lin-yao; LIU Yue-yue; TONG Xiu-bing; XIAO Si-yang; KAN Yu; FANG Yan-ping; JING Xiang-hong; LIAO Jun

doi:10.13703/j.0255-2930.20230327-k0003

您当前的位置：

首页 >

文章列表页 >

Effects of electroacupuncture on gait and proliferation and differentiation of muscle satellite cell in rats with acute blunt trauma of gastrocnemius muscle

Jingjin Golumn | 更新时间：2024-06-03

- Effects of electroacupuncture on gait and proliferation and differentiation of muscle satellite cell in rats with acute blunt trauma of gastrocnemius muscle
- Chinese Acupuncture & Moxibustion Vol. 43, Issue 9, Pages: 982-989(2023)
- 作者机构：
  
  1.福建中医药大学针灸学院，福州 350122
  2.中国中医科学院针灸研究所
- 作者简介：
- 基金信息：
- DOI：10.13703/j.0255-2930.20230327-k0003
  CLC： R 245.9⁺7
- Received：27 March 2023，
  
  Online First：01 August 2023，
  
  Published：12 September 2023
- 稿件说明：
移动端阅览
黄于婷,陈佳彦,郑林遥等.电针对腓肠肌急性钝挫伤大鼠步态及肌卫星细胞增殖分化的影响[J].中国针灸,2023,43(09):982-989.

HUANG Yu-ting,CHEN Jia-yan,ZHENG Lin-yao,et al.Effects of electroacupuncture on gait and proliferation and differentiation of muscle satellite cell in rats with acute blunt trauma of gastrocnemius muscle[J].Chinese Acupuncture & Moxibustion,2023,43(09):982-989.
黄于婷,陈佳彦,郑林遥等.电针对腓肠肌急性钝挫伤大鼠步态及肌卫星细胞增殖分化的影响[J].中国针灸,2023,43(09):982-989. DOI： 10.13703/j.0255-2930.20230327-k0003.

HUANG Yu-ting,CHEN Jia-yan,ZHENG Lin-yao,et al.Effects of electroacupuncture on gait and proliferation and differentiation of muscle satellite cell in rats with acute blunt trauma of gastrocnemius muscle[J].Chinese Acupuncture & Moxibustion,2023,43(09):982-989. DOI： 10.13703/j.0255-2930.20230327-k0003.

摘要

目的

观察电针对腓肠肌急性钝挫伤大鼠痛阈、步态及肌卫星细胞增殖分化的影响，探讨电针促进骨骼肌急性损伤修复的可能机制。

方法

将48只SD大鼠随机分为空白组（6只）、模型组（24只）和电针组（18只）。模型组、电针组大鼠采用自制打击器建立右侧腓肠肌急性钝挫伤模型。电针组大鼠于右侧“承山”“阳陵泉”行电针干预，予疏密波，频率2 Hz/100 Hz，每次30 min，每日1次。按取材时间分别干预3、7、14 d。造模后第1、3、7、14天，采用Von Frey法检测各组大鼠后足机械缩足反射痛阈，计算左右足痛阈差值；Cat Walk XT

动物步态分析仪记录大鼠站立时间及右后足最大接触面积；HE染色法观察大鼠右侧腓肠肌形态及炎性细胞数；免疫荧光法检测大鼠右侧腓肠肌配对盒基因7（Pax7）及成肌分化因子（MyoD）阳性表达。

结果

造模后右侧腓肠肌肌纤维断裂，可见大量红细胞及炎性细胞浸润；电针干预后肌纤维组织形态完整，炎性细胞浸润明显减少。与空白组比较，模型组大鼠造模后第1、3、7、14天后足机械缩足反射痛阈差值升高（

0.05），站立时间缩短、右后足最大接触面积减少（

0.05），右侧腓肠肌炎性细胞数及Pax7、MyoD阳性表达增加（

0.05）。与模型组比较，电针组大鼠造模后第7、14天后足机械缩足反射痛阈差值降低、站立时间延长（

0.05），右侧腓肠肌炎性细胞数减少（

0.05）；造模后第3、7、14天右后足最大接触面积增大、右侧腓肠肌MyoD阳性表达增加（

0.05）；造模后第3天右侧腓肠肌Pax7阳性表达增加（

0.05）。

结论

电针可有效改善腓肠肌急性钝挫伤大鼠痛阈及步态，促进骨骼肌损伤修复，其机制可能与增加Pax7、MyoD表达，从而促进肌卫星细胞增殖分化有关。

Abstract

Objective

To observe the effects of electroacupuncture on threshold of pain

gait

proliferation and differentiation of muscle satellite cell in rats with acute blunt trauma of gastrocnemius muscle

and to explore the possible mechanism of electroacupuncture in promoting the repair of acute injury of skeletal muscle.

Methods

A total of 48 SD rats were randomly divided into a blank group (6 rats)

a model group (24 rats) and an electroacupuncture group (18 rats). In the model group and the electroacupuncture group

the model of acute blunt trauma of gastrocnemius muscle was established by self-made impactor. In the electroacupuncture group

electroacupuncture was applied at "Chengshan" (BL 57) and "Yanglingquan" (GB 34) on the right side

with disperse-dense wave

in frequency of 2 Hz/100 Hz

once a day

30 min each time. Electroacupuncture intervention was performed for 3

7 and 14 days according to the sampling time. On the 1st

3rd

7th and 14th days after modeling

the mechanical withdrawal pain threshold of hindfoot was detected by Von Frey method; the standing time and the maximum contact area of the right hindfoot were recorded by Cat Walk XT

animal gait analysis instrument; the morphology of the right gastrocnemius muscle and the number of inflammatory cells were observed by HE staining; the positive expression of paired box gene 7 (Pax7) and myogenic differentiation (MyoD) of the right gastrocnemius muscle was detected by immunofluorescence.

Results

After modeling

the muscle fiber rupture and massive infiltration of red blood cells and inflammatory cells were observed in the right gastrocnemius muscle; after electroacupuncture intervention

the morphology of muscle fiber was intact and the infiltration of inflammatory cells was improved. Compared with the blank group

in the model group

the differences of mechanical withdrawal pain threshold between the left and right foot were increased (

0.05)

the standing time was shortened and the maximum contact area of the right hindfoot was decreased (

0.05)

the number of inflammatory cells and the positive expression of Pax7 and MyoD of the right gastrocnemius muscle were increased (

0.05) on the 1st

3rd

7th and 14th

days after modeling. Compared with the model group

in the electroacupuncture group

the differences of mechanical withdrawal pain threshold were decreased (

0.05)

the standing time was prolonged (

0.05)

the number of inflammatory cells of right gastrocnemius muscle was decreased (

0.05) on the 7th and 14th days after modeling; the maximum contact area of the right hindfoot was increased (

0.05)

the positive expression of MyoD of the right gastrocnemius muscle was increased (

0.05) on the 3rd

7th and 14th days after modeling; the positive expression of Pax7 of the right gastrocnemius muscle was increased (

0.05) on the 3rd day after modeling.

Conclusion

Electroacupuncture can effectively improve the pain threshold and gait in rats with acute blunt trauma of gastrocnemius muscle

and promote the repair of skeletal muscle injury

the mechanism may be related to the up-regulation of Pax7 and MyoD

so as to promoting the proliferation and differentiation of muscle satellite cell.

关键词

Keywords

references

Sanni AA , Blanks AM , Derella CC , et al . The effects of whole-body vibration amplitude on glucose metabolism, inflammation, and skeletal muscle oxygenation [J ] . Physiol Rep , 2022 , 10 ( 5 ): e15208 .

Zbinden-Foncea H , Castro-Sepulveda M , Fuentes J , et al . Effect of epicatechin on skeletal muscle [J ] . Curr Med Chem , 2022 , 29 ( 6 ): 1110 - 1123 .

Zhang HY , He FY , Zhou LF , et al . Activation of TLR4 induces inflammatory muscle injury via mTOR and NF-κB pathways in experimental autoimmune myositis mice [J ] . Biochem Biophys Res Commun , 2022 , 603 : 29 - 34 .

Carraro U , Kern H . Severely atrophic human muscle fibers with nuclear misplacement survive many years of permanent denervation [J ] . Eur J Transl Myol , 2016 , 26 ( 2 ): 5894 .

Enright K , Green M , Hay G , et al . Workload and injury in professional soccer players: role of injury tissue type and injury severity [J ] . Int J Sports Med , 2020 , 41 ( 2 ): 89 - 97 .

卢雅梦 , 雷静 , 尤浩军 . 骨骼肌损伤后疼痛机制及非药物治疗研究进展 [J ] . 中国疼痛医学杂志 , 2023 , 29 ( 2 ): 138 - 143 .

叶晔 . 急性骨骼肌损伤的外治法研究进展 [D ] . 北京 : 北京中医药大学 , 2008 .

孙峰 , 史晓林 , 李旭云 . 针刺配合中药熏蒸治疗急性腓肠肌损伤24例临床观察 [J ] . 甘肃中医药大学学报 , 2016 , 33 ( 1 ): 58 - 60 .

许建丰 . 阿是穴深刺加电针治疗臀中肌损伤综合征临床观察 [J ] . 内蒙古中医药 , 2020 , 39 ( 11 ): 125 - 127 .

孙阳 , 邵萍 , 郑晓 . 电针承山穴治疗急性腰扭伤临床疗效观察 [J ] . 辽宁中医杂志 , 2020 , 47 ( 4 ): 176 - 182 .

Boström P , Wu J , Jedrychowski MP , et al . A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis [J ] . Nature , 2012 , 481 ( 7382 ): 463 - 468 .

Ma XL , Du YR , Wang SL , et al . Adjacent segment degeneration after intervertebral fusion surgery by means of cervical block vertebrae [J ] . Eur Spine J , 2018 , 27 ( 6 ): 1401 - 1407 .

袁亚 , 朱世鹏 , 金洵 , 等 . 电针对腓肠肌急性钝挫伤大鼠肌肉弹性及生肌调节因子表达的影响 [J ] . 针刺研究 , 2020 , 45 ( 4 ): 287 - 292 .

中国针灸学会 . 实验动物常用穴位名称与定位第2部分:大鼠 [J ] . 针刺研究 , 2021 , 46 ( 4 ): 351 - 352 .

邹媛 , 刘通 , 李健 , 等 . 斜刺阿是穴对股四头肌损伤小鼠肌卫星细胞成肌分化抗原/生肌转录因子的影响 [J ] . 针刺研究 , 2021 , 46 ( 12 ): 1011 - 1015, 1022 .

Xiao WH , Liu Y , Luo BB , et al . Time-dependent gene expression analysis after mouse skeletal muscle contusion [J ] . J Sport Health Sci , 2016 , 5 ( 1 ): 101 - 108 .

李学艳 , 张春 , 陈默 , 等 . 大鼠爪印印迹分析用于骨关节炎疼痛模型评估 [J ] . 西南国防医药 , 2021 , 31 ( 2 ): 97 - 100 .

Heinzel J , Längle G , Oberhauser V , et al . Use of the CatWalk gait analysis system to assess functional recovery in rodent models of peripheral nerve injury - a systematic review [J ] . J Neurosci Methods , 2020 , 345 : 108889 .

Miyagi M , Ishikawa T , Kamoda H , et al . Assessment of gait in a rat model of myofascial inflammation using the CatWalk system [J ] . Spine , 2011 , 36 ( 21 ): 1760 - 1764 .

Tomar L , Govil G , Dhawan P . Bilateral femoral neck stress fracture in an obese middle-aged female with osteomalacia and coxa-Vara managed by simultaneous bilateral total hip arthroplasty [J ] . Cureus , 2020 , 12 ( 11 ): e11478 .

邢华医 , 周谋望 . 骨骼肌卫星细胞的增殖分化及其影响因素 [J ] . 中国康复医学杂志 , 2016 , 31 ( 1 ): 114 - 117 .

Rudnicki MA , Le Grand F , McKinnell I , et al . The molecular regulation of muscle stem cell function [J ] . Cold Spring Harb Symp Quant Biol , 2008 , 73 : 323 - 331 .

von Maltzahn J , Jones AE , Parks RJ , et al . Pax7 is critical for the normal function of satellite cells in adult skeletal muscle [J ] . Proc Natl Acad Sci USA , 2013 , 110 ( 41 ): 16474 - 16479 .

Tian ZL , Jiang SK , Zhang M , et al . Detection of satellite cells during skeletal muscle wound healing in rats: time-dependent expressions of Pax7 and MyoD in relation to wound age [J ] . Int J Legal Med , 2016 , 130 ( 1 ): 163 - 172 .

Olguin HC , Yang ZH , Tapscott SJ , et al . Reciprocal inhibition between Pax7 and muscle regulatory factors modulates myogenic cell fate determination [J ] . J Cell Biol , 2007 , 177 ( 5 ): 769 - 779 .

Le Grand F , Rudnicki M . Satellite and stem cells in muscle growth and repair [J ] . Development , 2007 , 134 ( 22 ): 3953 - 3957 .

李俊华 , 王正珍 , 王军力 , 等 . 针刺对急性骨骼肌钝挫伤大鼠肌卫星细胞增殖的影响 [J ] . 中国体育科技 , 2012 , 48 ( 4 ): 137 - 140 .

李永平 , 梁炳生 . MyoD肌形成作用机制研究进展 [J ] . 国际骨科学杂志 , 2007 , 28 ( 1 ): 37 - 40 .

Khachian A , Seyedoshohadaei M , Haghani H , et al . Effect of self-management program on outcome of adult knee osteoarthritis [J ] . Int J Orthop Trauma Nurs , 2020 , 39 : 100797 .

孙文静 , 马惠昇 , 穆静 , 等 . 理筋手法通过PI3K-Akt通路干预兔静力性损伤的机制研究 [J ] . 江苏中医药 , 2022 , 54 ( 11 ): 72 - 76 .

唐芳 , 高品操 , 潘同斌 . 钝挫伤对大鼠骨骼肌卫星细胞体外增殖的影响 [J ] . 中国老年学杂志 , 2017 , 37 ( 5 ): 1091 - 1093 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of electroacupuncture on denervated skeletal muscle atrophy in rats based on p38 MAPK signaling pathway

Effect of electroacupuncture on microglial activation in the lumbar spinal cord of rabbits with lumbar intervertebral disc degeneration

Electroacupuncture promotes browning of white adipose tissue by regulating mTOR/SREBP1 signaling pathway in obese mice

Mechanism of "biaoben acupoint combination" electroacupuncture pretreatment in alleviating cardiomyocyte cuproptosis in rats with myocardial ischemia-reperfusion injury

Clinical efficacy and cost-effectiveness evaluation of acupoint thread-embedding for knee osteoarthritis of early to middle stage

Related Author

QIU Wei

TANG Chenglin

LIAO Cai

YANG Yunhao

YANG Yan

YANG Kang

PENG Wanchun

YANG Jiawang

Related Institution

College of TCM, Chongqing Medical University, Chongqing Key Laboratory of TCM for Prevention and Treatment of Metabolic Diseases

Department of Orthopedics, Guangyuan Second People's Hospital

Department of General Medicine, Guangyuan Maternal and Child Health Hospital

Treatment Center of Acupuncture and Moxibustion, Hubei Provincial Hospital of TCM

Treatment Center of Acupuncture and Moxibustion, Affiliated Hospital of Hubei University of CM

⁰