Objective

2

To explore the rules of acupoint selection and pattern-acupoint relationship in treatment with acupuncture and moxibustion for endometriosis (EMs) based on complex network analysis technology.

Methods

2

The articles for clinical trial of EMs treated with acupuncture and moxibustion were searched from CNKI

Wanfang

VIP

SinoMed

PubMed

EMbase and Cochrane Library from the inception of the databases to December 14

2022. Using Microsoft Excel 2019 software

the database was established to collect the use frequency of acupoint

meridian tropism

location and pattern-acupoint relationship. SPSS Modeler 18.0 Apriori algorithm was adopted to conduct the association rule analysis

Cytoscape3.7.2 software was used to plot the complex co-occurrence network map; and SPSS Statistics 26.0 was adopted to perform hierarchical cluster analysis on high-frequency acupoints and a tree diagram was drawn.

Results

2

A total of 163 articles were included

and 167 core acupoint prescriptions and 74 pattern-associated acupoint prescriptions were extracted

involving 92 acupoints

with a cumulative frequency of 1 223 times. The top five acupoints with the highest use frequency were Guanyuan (CV 4)

Sanyinjiao (SP 6)

Zhongji (CV 3)

Zigong (EX-CA 1) and Qihai (CV 6). The selected acupoints were mostly distributed in the chest

abdomen and lower limbs; and the involved meridians included the conception vessel

the spleen meridian of foot-

taiyin

and the stomach meridian of foot-

yangming

. The acupoint compatibility of high fr

equency referred to Guanyuan (CV 4) - Sanyinjiao (SP 6)

Guanyuan (CV 4) - Zhongji (CV 3)

and Guanyuan (CV 4) - Zigong (EX-CA 1). The close association was presented among Guanyuan (CV 4)

Sanyinjiao (SP 6)

Qihai (CV 6) and Zhongji (CV 3)

which had the strongest connection with the other acupoints; among the top 25 acupoints with the highest use frequency

5 acupoint prescriptions with high frequency were obtained by the cluster analysis. Guanyuan (CV 4)

Qihai (CV 6)

Sanyinjiao (SP 6)

Zigong (EX-CA 1) and Zhongji (CV 3) were selected for cold and blood stagnation; Guanyuan (CV 4)

Sanyinjiao (SP 6)

Zhongji (CV 3)

Dahe (KI 12) and Taixi (KI 3) for kidney deficiency and blood stagnation; Zhongji (CV 3)

Guanyuan (CV 4)

Sanyinjiao (SP 6)

Xuehai (SP 10) and Diji (SP 8) for

qi

and blood stagnation; Qihai (CV 6)

Guanyuan (CV 4)

Zusanli (ST 36)

Xuehai (SP 10)

and Zigong (EX-CA 1) for

qi

deficiency and blood stagnation; Sanyinjiao (SP 6)

Fenglong (ST 40)

Zhongliao (BL 33)

Ciliao (BL 32) and Xialiao (BL 34) for interaction of phlegm and stasis; and Daheng (SP 15)

Guanyuan (CV 4)

Zhongji (CV 3)

Qihai (CV 6) and Zhongwan (CV 12) for retention of damp and heat.

Conclusion

2

The core acupoints are Guanyuan (CV 4)

Sanyinjiao (SP 6)

Zhongji (CV 3)

Qihai (CV 6) and Zigong (EX-CA 1) in treatment of endometriosis with acupuncture and moxibustion. Six patterns/syndromes are involved in clinical practice. In terms of the properties

functions and indications

the supplementary acupoints are selected on the basis of the core acupoints for different patterns/sydnromes of the disease.