Effect of electroacupuncture at “Neiguan” (PC 6) and “Zusanli” (ST 36) on gastrointestinal hormone in the antral tissue of rats with functional dyspepsia

XIAO Yi; ZHOU Jing-ying; YIN Hong-zhi; LIU Qian; LUO Rong; LIU Wei-ai; DU Ge-shu; ZHONG Huan; LIU Mi

doi:10.13703/j.0255-2930.20230809-0007

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Effect of electroacupuncture at “Neiguan” (PC 6) and “Zusanli” (ST 36) on gastrointestinal hormone in the antral tissue of rats with functional dyspepsia

Study on Mechanism | 更新时间：2024-06-03

- Effect of electroacupuncture at “Neiguan” (PC 6) and “Zusanli” (ST 36) on gastrointestinal hormone in the antral tissue of rats with functional dyspepsia
- Chinese Acupuncture & Moxibustion Vol. 43, Issue 12, Pages: 1435-1440(2023)
- 作者机构：
  
  1.湖南中医药大学针灸推拿与康复学院，长沙 410208
  2.湖南中医药大学第一附属医院针灸推拿康复科
  3.湖南中医药大学第二附属医院针灸推拿康复科
  4.长沙市中医医院针灸康复科
- 作者简介：
- 基金信息：
- DOI：10.13703/j.0255-2930.20230809-0007
  CLC： R245.9⁺7
- Received：09 August 2023，
  
  Online First：30 November 2023，
  
  Published：12 December 2023
- 稿件说明：
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肖逸,周竞颖,尹鸿智等.电针“内关”“足三里”对功能性消化不良大鼠胃窦组织胃肠激素的影响[J].中国针灸,2023,43(12):1435-1440.

XIAO Yi,ZHOU Jing-ying,YIN Hong-zhi,et al.Effect of electroacupuncture at “Neiguan” (PC 6) and “Zusanli” (ST 36) on gastrointestinal hormone in the antral tissue of rats with functional dyspepsia[J].Chinese Acupuncture & Moxibustion,2023,43(12):1435-1440.
肖逸,周竞颖,尹鸿智等.电针“内关”“足三里”对功能性消化不良大鼠胃窦组织胃肠激素的影响[J].中国针灸,2023,43(12):1435-1440. DOI： 10.13703/j.0255-2930.20230809-0007.

XIAO Yi,ZHOU Jing-ying,YIN Hong-zhi,et al.Effect of electroacupuncture at “Neiguan” (PC 6) and “Zusanli” (ST 36) on gastrointestinal hormone in the antral tissue of rats with functional dyspepsia[J].Chinese Acupuncture & Moxibustion,2023,43(12):1435-1440. DOI： 10.13703/j.0255-2930.20230809-0007.

摘要

目的

观察电针“内关”“足三里”对功能性消化不良（FD）大鼠胃排空率、胃窦组织5-羟色胺（5-HT）含量及胃动素（MTL）、胃饥饿素（Ghrelin）、P物质（SP）、血管活性肠肽（VIP）蛋白表达的影响，探讨电针治疗FD的作用机制。

方法

将21只SPF级雄性SD幼鼠随机分为正常组、模型组、电针组，每组7只。模型组、电针组大鼠以0.1 %蔗糖碘乙酰胺溶液灌胃叠加改良小平台法制备FD模型。造模结束后，电针组予电针“内关”“足三里”干预，疏密波，频率20 Hz /100 Hz，每次30 min，每日1次，持续7 d。干预前后，观察大鼠一般情况；干预结束后，计算大鼠胃排空率，采用HE染色法观察大鼠胃窦组织形态，ELISA法检测胃窦组织5-HT含量，Western blot法检测胃窦组织MTL、Ghrelin、SP、VIP蛋白表达。

结果

正常组大鼠精神状态良好，毛发润泽，活动灵敏，饮食量、体质量增加；模型组大鼠精神状态差，毛发无光泽，喜蜷缩，活动度、反应度降低，部分大鼠大便稀溏，胃体明显增大，胃内有食物残留；电针组大鼠精神状态、饮食量、活动度等一般情况较模型组改善，胃体较模型组明显缩小，胃内食物残留减少。各组大鼠胃窦组织结构完整，腺体丰富，胃黏膜未见明显炎性反应、水肿等损伤。与正常组比较，模型组大鼠胃排空率下降（

0.01），5-HT含量升高（

0.01），MTL、Ghrelin蛋白表达下降（

0.01），VIP蛋白表达升高（

0.01）。与模型组比较，电针组大鼠胃排空率升高（

0.01），5-HT含量下降（

0.01），MTL、Ghrelin蛋白表达升高（

0.05，

0.01）。

结论

电针 “内关”“足三里”可有效改善FD大鼠胃肠动力障碍，增强胃运动，促进胃排空，从而缓解消化不良症状，其机制可能与调节胃窦组织胃肠激素水平有关。

Abstract

Objective

To observe the effects of electroacupuncture (EA) at “Neiguan” (PC 6) and “Zusanli”(ST 36) on the gastric emptying rate

the level of serotonin (5-HT) and the protein expression of motilin (MTL)

ghrelin

substance P (SP) and vasoactive intestinal peptide (VIP) in the antral tissue of the rats with functional dyspepsia (FD) and explore the effect mechanism of EA in treatment of FD.

Methods

A total of 21 SPF male SD rat pups were randomly divided into a normal group

a model group and an EA group

with 7 rats in each group. In the model group and the EA group

FD model was prepared by the gavage with 0.1% sucrose iodoacetamide solution combined with the modified small platform method. After the successful modeling

EA was applied to “Neiguan” (PC 6) and “Zusanli”(ST 36) in the rats of the EA group

with disperse-dense wave

20 Hz/100 Hz in frequency

stimulated for 30 min

once daily

for 7 days consecutively. Before and after intervention

the general condition of the rats was observed in each group. After the completion of intervention

the gastric emptying rate was measured

the morphological changes of gastric antral tissue were observed using HE staining

the level of 5-HT was detected with ELISA method

and the protein expression of MTL

ghrelin

and VIP was determined with Western blot method in the antral tissue of rats.

Results

In the normal group

the rats were in a good mental state

with lustrous fur

flexible movement and the increase of food intake and body mass. In the model group

the rats were poor in mental state

lack of lustre in fur

preference for the body curled up

reduced activity and response; and a part of rats had loose stool

obviously enlarged gastric body and gastric food retention. In the EA group

the general condition of rats

e.g. the mental state

food intake and activity

were improved

the gastric body got smaller obviously and the gastric food retention was reduced when compared with the model group. The antral structure was intact

the glands were rich and no injury of the gastric mucosa was found

e.g. inflammatory reaction and edema in the rats of each group. Compared with the normal group

the gastric emptying rate was decreased (

0.01)

5-HT level was increased (

0.01)

the protein expression of MTL and ghrelin was reduced (

0.01) and that of VIP was elevated (

0.01) in the rats of the model group. The gastric emptying rate was increased (

0.01)

5-HT level was decreased (

0.01)

and the protein expression of MTL and ghrelin was elevated (

0.05

0.01) in the rats of the EA group when compared with those in the model group.

Conclusion

Electroacupuncture at “Neiguan” (PC 6) and “Zusanli”(ST 36) may effectively relieve gastric dysfunction

strengthen gastric motility and promote gastric emptying so as to alleviate the symptoms of dyspepsia in FD rats

and its mechanism may be related to the regulation of gastrointestinal hormones in the antral tissue.

关键词

Keywords

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