Effects of electroacupuncture at pterygopalatine region on NLRP3-mediated pyroptosis and inflammatory factors in allergic rhinitis rats

LV Haiyang; TIAN Meihui; SHE Shuyi; LIU Yucheng; SUN Lei; SONG Wu; TANG Yong

doi:10.13703/j.0255-2930.20240204-k0001

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Effects of electroacupuncture at pterygopalatine region on NLRP3-mediated pyroptosis and inflammatory factors in allergic rhinitis rats

Study on Mechanism | 更新时间：2025-03-13

- Effects of electroacupuncture at pterygopalatine region on NLRP3-mediated pyroptosis and inflammatory factors in allergic rhinitis rats
- Chinese Acupuncture & Moxibustion Vol. 45, Issue 3, Pages: 345-350(2025)
- 作者机构：
  
  长春中医药大学，吉林长春 130117
  长春中医药大学附属第三临床医院
  长春中医药大学临床医学院，吉林长春 130117
  长春中医药大学附属医院，吉林长春 130021
- 作者简介：
- 基金信息：
- DOI：10.13703/j.0255-2930.20240204-k0001
  CLC：
- Received：04 February 2024，
  
  Online First：29 November 2024，
  
  Published：12 March 2025
- 稿件说明：
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LV Haiyang, TIAN Meihui, SHE Shuyi, et al. Effects of electroacupuncture at pterygopalatine region on NLRP3-mediated pyroptosis and inflammatory factors in allergic rhinitis rats[J]. Chinese Acupuncture & Moxibustion, 2025, 45(3): 345-350.
DOI：

LV Haiyang, TIAN Meihui, SHE Shuyi, et al. Effects of electroacupuncture at pterygopalatine region on NLRP3-mediated pyroptosis and inflammatory factors in allergic rhinitis rats[J]. Chinese Acupuncture & Moxibustion, 2025, 45(3): 345-350. DOI： 10.13703/j.0255-2930.20240204-k0001.

摘要

目的：

观察电针翼腭区对变应性鼻炎（AR）大鼠NOD样受体热蛋白结构域相关蛋白3（NLRP3）介导的细胞焦亡和炎性因子的影响。

方法：

将24只SD大鼠随机分为空白组、模型组、针刺组、电针组，每组6只。除空白组外，余组大鼠均经卵清白蛋白（OVA）致敏法建立AR大鼠模型。电针组大鼠予双侧翼腭区电针治疗，选择疏密波，频率2 Hz/100 Hz，电流0.5～1 mA，每次15 min，隔日1次，共3次；针刺组大鼠仅进行双侧翼腭区针刺，不连接电针。观察各组大鼠的鼻炎症状积分，HE染色观察大鼠鼻黏膜组织形态，ELISA法检测大鼠血清OVA特异性免疫球蛋白E（OVA-sIgE）、白细胞介素（IL）-4、IL-6和IL-1β的含量，实时荧光定量PCR法检测鼻黏膜中NLRP3、凋亡相关斑点样蛋白（ASC）、半胱氨酸天冬氨酸蛋白酶-1（caspase-1）、IL-18的mRNA表达，Western blot法检测大鼠鼻黏膜中NLRP3、ASC、caspase-1、IL-18的蛋白表达。

结果：

与空白组比较，模型组大鼠鼻炎症状积分升高（

0.01），血清OVA-sIgE、IL-4、IL-6、IL-1β含量均升高（

0.05），鼻黏膜呈炎性浸润病理表现，且鼻黏膜组织中的NLRP3、ASC、caspase-1、IL-18的mRNA及蛋白表达升高（

0.05）；与模型组比较，电针组大鼠鼻炎症状积分降低（

0.01），鼻黏膜病理表现改善，血清OVA-sIgE、IL-4、IL-6和IL-1β的含量降低（

0.05），鼻黏膜中的NLRP3、ASC、caspase-1、IL-18 mRNA及蛋白表达降低（

0.05）。

结论：

电针翼腭区可通过抑制NLRP3介导的细胞焦亡和炎性因子失衡来发挥抗炎作用，从而缓解AR大鼠的鼻炎症状。

Abstract

Objective

To observe the effects of electroacupuncture at the pterygopalatine region on nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3)-mediated pyroptosis and inflammatory factors in rats with allergic rhinitis (AR).

Methods

Twenty-four SD rats were randomly divided into a blank group

a model group

an acupuncture group and an electroacupuncture group

6 rats in each group. Except for the blank group

OVA-induced AR model was established in the remaining groups. In the electroacupuncture group

the rats were treated with electroacupuncture at the bilateral pterygopalatine region

with disperse-dense wave

in frequency of 2 Hz/100 Hz and current of 0.5-1 mA

15 min each time

once every other day

for 3 times. In the acupuncture group

the rats were treated with acupuncture at bilateral pterygopalatine region simply

without electrical stimulation. The rhinitis symptom score was observed

the pathomorphology of the nasal mucosa was observed by HE staining; the serum levels of OVA-specific immunoglobulin E (OVA-sIgE)

interleukin (IL)-4

IL-6 and IL-1β were detected by ELISA; the mRNA expression of NLRP3

apoptosis-associated speck-like protein containing CARD (ASC)

cysteinyl aspartate specific proteinase-1 (caspase-1) and IL-18 in the nasal mucosa was detected by real-time PCR; the protein expression of NLRP3

ASC

caspase-1 and IL-18 in the nasal mucosa was detected by Western blot.

Results

Compared with the blank group

in the model group

the rhinitis symptom score was increased (

0.01)

the serum levels of OVA-sIgE

IL-4

IL-6 and IL-1β were increased (

0.05)

the nasal mucosa showed pathomorphology of inflammatory infiltration; the mRNA and protein expression of NLRP3

ASC

caspase-1 and IL-18 in the nasal mucosa was increased (

0.05). Compared with the model group

in the electroacupuncture group

the rhinitis symptom score was reduced (

0.01)

the pathology of the nasal mucosa was improved; the serum levels of OVA-sIgE

IL-4

IL-6 and IL-1β were decreased (

0.05); the mRNA and protein expression of NLRP3

ASC

caspase-1 and IL-18 in the nasal mucosa was decreased (

0.05).

Conclusion

Electroacupuncture at the pterygopalatine region can exerting the anti-inflammatory effect by inhibiting NLRP3-mediated pyroptosis and inflammatory factor imbalance

thus alleviate rhinitis symptoms in AR rats.

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