Effect of moxibustion at "Shenque" (CV8) on the expression of BDNF and c-fos in the urinary control brain regions of rats with neurogenic bladder after spinal cord injury

YU Han; FU Yuanbo; LIU Huilin; ZHANG Yuzhuo; NI Yutong; LI Qingdai; XU Yi

doi:10.13703/j.0255-2930.20240507-k0004

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Effect of moxibustion at "Shenque" (CV8) on the expression of BDNF and c-fos in the urinary control brain regions of rats with neurogenic bladder after spinal cord injury

Study on Mechanism | 更新时间：2025-05-15

- Effect of moxibustion at "Shenque" (CV8) on the expression of BDNF and c-fos in the urinary control brain regions of rats with neurogenic bladder after spinal cord injury
- Chinese Acupuncture & Moxibustion Vol. 45, Issue 5, Pages: 638-645(2025)
- 作者机构：
  
  北京中医药大学，北京　100029
  首都医科大学附属北京中医医院针灸科，北京　100010
- 作者简介：
- 基金信息：
- DOI：10.13703/j.0255-2930.20240507-k0004
  CLC：
- Received：07 May 2024，
  
  Online First：19 February 2025，
  
  Published：12 May 2025
- 稿件说明：
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YU Han, FU Yuanbo, LIU Huilin, et al. Effect of moxibustion at "Shenque" (CV8) on the expression of BDNF and c-fos in the urinary control brain regions of rats with neurogenic bladder after spinal cord injury[J]. Chinese Acupuncture & Moxibustion, 2025, 45(5): 638-645.
DOI：

YU Han, FU Yuanbo, LIU Huilin, et al. Effect of moxibustion at "Shenque" (CV8) on the expression of BDNF and c-fos in the urinary control brain regions of rats with neurogenic bladder after spinal cord injury[J]. Chinese Acupuncture & Moxibustion, 2025, 45(5): 638-645. DOI： 10.13703/j.0255-2930.20240507-k0004.

摘要

目的：

观察艾灸“神阙”对脊髓损伤后神经源性膀胱（NB）大鼠尿动力学及脑桥排尿中枢（PMC）、中脑导水管周围灰质（PAG）、内侧前额叶皮层（mPFC）脑源性神经营养因子（BDNF）及即刻早期基因c-fos表达的影响。

方法：

将24只SPF级雌性SD大鼠随机分为假手术组（6只）和脊髓横断组（18只），脊髓横断组大鼠采用T

完全脊髓横断法建立神经源性逼尿肌过度活动模型，将造模成功的12只大鼠随机分为模型组和艾灸组，每组

6只。艾灸组大鼠予隔姜隔盐灸“神阙”，每次连续灸4壮，每日1次，共干预14 d。干预后，检测各组大鼠尿动力学指标，采用实时荧光定量PCR法检测大鼠PMC、PAG、mPFC中BDNF、c-fos mRNA表达，采用Western blot法检测大鼠PMC、PAG、mPFC中BDNF、c-fos蛋白表达。

结果：

假手术组大鼠在膀胱充盈期间未出现逼尿肌期相性收缩。与模型组比较，艾灸组大鼠漏尿前5 min期相性收缩频率、波幅均降低（

0.05），膀胱充盈期间期相性收缩首次出现时间延长（

0.05）。与假手术组比较，模型组大鼠PMC、PAG、mPFC中BDNF、c-fos mRNA及蛋白表达升高（

0.05）；与模型组比较，艾灸组大鼠PMC、PAG、mPFC中BDNF、c-fos mRNA及蛋白表达降低（

0.05）。

结论：

艾灸“神阙”可改善脊髓损伤后NB大鼠膀胱充盈期间期相性收缩情况，可能通过下调PMC、PAG、mPFC中BDNF、c-fos mRNA及蛋白表达实现。

Abstract

Objective

To observe the effects of moxibustion at "Shenque" (CV8) on urodynamics and the expression of brain-derived neurotrophic factor (BDNF) and immediate early gene (c-fos) in pontine micturition center (PMC)

periaqueductal gray (PAG)

medial prefrontal cortex (mPFC) of neurogenic bladder (NB) rats after spinal cord injury.

Methods

Twenty-four SPF female SD rats were randomly divided into a sham-operation group (6 rats) and a modeling group (18 rats). In the modeling group

complete spinal cord transection method was used to establish a neurogenic detrusor overactivity model

and the 12 rats with successful modeling were randomized into a model group and a moxibustion group

with 6 rats in each group. The rats in the moxibustion group were treated with ginger/salt-insulated moxibustion at "Shenque" (CV8)

and 4 consecutive moxa cones were delivered in one intervention. Moxibustion was operated once daily and for 14 days. After intervention completion

the urodynamic indexes of rats in each group were detected. Fluorescence quantitative PCR was used to detect the mRNA expression of BDNF and c-fos in PMC

PAG and mPFC in rats. Western blot was used to detect the protein expression of BDNF and c-fos in PMC

PAG and mPFC.

Results

The rats in the sham-operation grou

p did not show phasic detrusor contraction during bladder filling. Compared with the model group

the frequency and amplitude of the phasic detrusor contraction were reduced 5 min before urine leakage in the rats of the moxibustion group (

0.05)

and the duration of the first phasic detrusor contraction during bladder filling was prolonged (

0.05). Compared with the sham-operation group

the mRNA and protein expression of BDNF and c-fos in PMC

PAG and mPFC increased in the model group (

0.05). Compared with the model group

the mRNA and protein expression of BDNF and c-fos in PMC

PAG and mPFC decreased in the moxibustion group (

0.05).

Conclusion

Moxibustion at "Shenque" (CV8) can improve the phasic contraction during bladder filling in NB rats after spinal cord injury

possibly by down-regulating the mRNA and protein expression of BDNF and c-fos in PMC

PAG

and mPFC.

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