Effects of <italic style="font-style: italic">Tongdu Tiaoshen</italic> acupuncture on depression-like behavior and Endophilin A1/ROS pathway in hippocampal tissue of CUMS model rats

ZOU Ling; SONG Xiaoge; ZHAO Yanbiao; QIAN Tingting; CHU Yifan; PAN Wen; CHU Haoran; YANG Shaojie; SUN Meixiang; SUN Peiyang

doi:10.13703/j.0255-2930.20240922-k0001

您当前的位置：

首页 >

文章列表页 >

Effects of Tongdu Tiaoshen acupuncture on depression-like behavior and Endophilin A1/ROS pathway in hippocampal tissue of CUMS model rats

Study on Mechanism | 更新时间：2025-09-12

- Effects of Tongdu Tiaoshen acupuncture on depression-like behavior and Endophilin A1/ROS pathway in hippocampal tissue of CUMS model rats
- Chinese Acupuncture & Moxibustion Vol. 45, Issue 9, Pages: 1281-1289(2025)
- 作者机构：
  
  安徽中医药大学第二附属医院脑病七科，合肥　230061
  安徽中医药大学针灸经络研究所
- 作者简介：
- 基金信息：
- DOI：10.13703/j.0255-2930.20240922-k0001
  CLC：
- Received：22 September 2024，
  
  Online First：03 December 2024，
  
  Published：12 September 2025
- 稿件说明：
移动端阅览
ZOU Ling, SONG Xiaoge, ZHAO Yanbiao, et al. Effects of Tongdu Tiaoshen acupuncture on depression-like behavior and Endophilin A1/ROS pathway in hippocampal tissue of CUMS model rats[J]. Chinese Acupuncture & Moxibustion, 2025, 45(9): 1281-1289. DOI: 10.13703/j.0255-2930.20240922-k0001.
DOI：

ZOU Ling, SONG Xiaoge, ZHAO Yanbiao, et al. Effects of Tongdu Tiaoshen acupuncture on depression-like behavior and Endophilin A1/ROS pathway in hippocampal tissue of CUMS model rats[J]. Chinese Acupuncture & Moxibustion, 2025, 45(9): 1281-1289. DOI: 10.13703/j.0255-2930.20240922-k0001. DOI：

摘要

目的

观察“通督调神”针刺对慢性不可预知温和刺激（CUMS）模型大鼠抑郁样行为及海马内吞蛋白A1（Endophilin A1）/活性氧（ROS）信号通路的影响，探讨“通督调神”针刺治疗抑郁症的作用机制。

方法

将48只SPF级雄性SD大鼠随机分为正常组（12只）、造模组（36只）。造模组采用CUMS法建立抑郁症大鼠模型。将造模成功的36只大鼠随机分为模型组、通督调神针刺组（简称针刺组）、氟西汀组，每组12只。针刺组予“通督调神”针刺，穴取“百会”“神庭”“水沟”“大椎”，每天1次，连续干预6 d后休息1 d，共干预28 d。氟西汀组大鼠予氟西汀溶液（2.1 mg/kg）灌胃，每天1次，连续干预28 d。于造模前、造模后、干预后观察各组大鼠体质量、蔗糖偏好率及旷场实验运动总距离、穿越方格数；干预后，采用HE法观察大鼠海马神经元形态，尼氏染色法观察大鼠海马尼氏小体，透射电镜观察大鼠海马线粒体情况，流式细胞术检测大鼠海马ROS平均荧光强度，Western blot法检测大鼠海马Endophilin A1、生长相关蛋白43（GAP-43）、脑源性神经营养因子（BDNF）蛋白表达，RT-qPCR法检测大鼠海马Endophilin A1、GAP-43、BDNF mRNA表达，免疫荧光法检测大鼠海马Endophilin A1、GAP-43、BDNF平均荧光强度。

结果

与正常组比较，模型组大鼠体质量、蔗糖偏好率及旷场实验运动总距离、穿越方格数降低（

0.01）；海马神经元结构不清晰，基质相对疏松，尼氏小体数量减少（

0.01）；海马线粒体结构紊乱、外膜破裂，线粒体嵴不规则或缺失；海马ROS平均荧光强度及Endophilin A1蛋白表达、mRNA表达、平均荧光强度升高（

0.01），GAP-43及BDNF蛋白表达、mRNA表达、平均荧光强度降低（

0.01）。与模型组比较，针刺组、氟西汀组大鼠体质量、蔗糖偏好率及旷场实验运动总距离、穿越方格数升高（

0.05，

0.01）；海马神经元结构相对清晰，基质相对致密，尼氏小体数量增多（

0.01）；海马线粒体结构稍正常，排列稍紊乱；海马ROS平均荧光强度及Endophilin A1蛋白表达、mRNA表达、平均荧光强度降低（

0.01），GAP-43及BDNF蛋白表达、mRNA表达、平均荧光强度升高（

0.01，

0.05）。与氟西汀组比较，针刺组大鼠海马ROS平均荧光强度、Endophilin A1蛋白表达及平均荧光强度、GAP-43蛋白表达、BDNF mRNA表达升高（

0.01，

0.05），BDNF蛋白表达及平均荧光强度、Endophilin A1 mRNA表达、GAP-43平均荧光强度降低（

0.01，

0.05）。

结论

“通督调神”针刺可减轻CUMS模型大鼠抑郁样行为，保护海马神经元，其机制可能与抑制Endophilin A1/ROS信号通路，减轻氧化应激反应有关。

Abstract

Objective

To observe the effects of

Tongdu Tiaoshen

acupuncture (for unblocking the obstruction in the governor vessel and regulating the spirit) on depression-like behavior and the hippocampal Endophilin A1/reactive oxygen species (ROS) pathway in the chronic unpredictable mild stress (CUMS) model rats

and explore the mechanism of this therapy for depression.

Methods

Forty-eight male SD rats of SPF grade were randomly divided into a normal group (

=12) and a modeling group (

=36). In the modeling group

CUMS was performed to establish depression model. The successfully-modeled rats were randomized into a model group

a Tongdu Tiaoshen acupuncture group (referred to as the acupuncture group)

and a fluoxetine group

with 12 rats in each group. In the acupuncture group

"Baihui" (GV20)

"Shenting" (GV24)

"Shuigou" (GV26) and "Dazhui" (GV14) were stimulated with acupuncture. This intervention measure was delivered once a day

continuously for 6 days; it was discontinued on day 7 and was completed in 28 days. In the fluoxetine group

intragastric administration was done with fluoxetine solution (2.1 mg/kg)

once a day

and for 28 consecutive days. Before and after modeling

and after intervention completion

the body mass

sucrose preference rate and the total distance of movement and the boxes of horizontal crossing in the open field experiment were observed in each group. After intervention

using HE staining

the hippocampal neuron morphology was observed; using Nissl staining

the hippocampal Nissl body number was counted. The hippocampal mitochondria was observed under transmission electron microscopy. The average fluorescence intensity of ROS in hippocampal was determined using flow cytometry. With Western blot method

the protein expression of Endophilin A1

growth associated protein 43 (GAP-4

and brain-derived neurotrophic factor (BDNF) in hippocampal was detected; and with RT-qPCR method

the mRNA expression of Endophilin A1

GAP-43

and BDNF was recorded. Using the immunofluorescence

the average fluorescence intensity of Endophilin A1

GAP-43

and BDNF in hippocampal tissue was determined.

Results

Compared with the normal group

in the model group

the body mass

sucrose preference rate

and the total distance of movement and the boxes of horizontal crossing in the open field experiment decreased (

0.01); the hippocampal neuronal structure was unclear

the matrix was relatively loose

and the number of Nissl body decreased (

0.01); mitochondrial structure was disarranged

the outer membrane was ruptured

mitochondrial cristae was irregular or missed; the average fluorescence intensity of ROS in hippocampal tissue

the protein and mRNA expression and the average fluorescence intensity of Endophilin A1 in hippocampal tissue increased (

0.01)

while the protein and mRNA expression of GAP-43 and BDNF and its average fluorescence intensity decreased (

0.01). Compared with the model group

the acupuncture group and the fluoxetine group showed the increase in body mass

sucrose preference rate

the total distance of movement and the boxes of horizontal crossing in the open field experiment (

0.05

0.01); the hippocampal neuronal structure became relatively clear

the matrix was relatively dense

and the number of Nissl body was elevated (

0.01); mitochondrial structure got normal and disarranged slightly

the average fluorescence intensity of ROS in hippocampal tissue

the protein and mRNA expression and the average fluorescence intensity of Endophilin A1 in hippocampal tissue were reduced (

0.01)

while the protein and mRNA expression of GAP-43 and BDNF and the average fluorescence intensity rose (

0.01

0.05). Compared with the fluoxetine group

the acupuncture group presented the increase in the average fluorescence intensity of ROS

the protein expression and the average fluorescence intensity of Endophilin A1

the protein expression of GAP-43 and the mRNA expression of BDNF (

0.01

0.05)

and the decrease of the protein expression and the average fluorescence intensity of BDNF

the mRNA expression of Endophilin A1

and the average fluorescence intensity of GAP-43 (

0.01

0.05).

Conclusion

Tongdu tiaoshen

acupuncture alleviates depression-like behaviors in CUMS model rats and protects hippocampal neurons

which may be related to suppressing Endophilin A1/ROS signaling pathway and attenuating oxidative stress reactions.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Tongdu Tiaoshen acupuncture on neuroinflammation in rats with CUMS based on the miR-26a-5p/EZH2/H3K27me3 pathway

Effect of Tongdu Tiaoshen acupuncture on hippocampal neuronal ferroptosis in depression rats based on SLC7A11/GPX4 pathway

Effect of electroacupuncture at "Hegu" (LI4) and "Taichong" (LR3) on DNA methylation of the SLC6A4 gene promoter in the hippocampus of depressed rats

Tiaoshen Guben holistic therapy of acupuncture and moxibustion for 24 cases of comorbidity of depression and insomnia

Evaluation of the reporting quality of randomized controlled trials of acupuncture for depression based on CONSORT statement, STRICTA criteria and SHARE checklist

Related Author

SUN Peiyang

PAN Wen

ZHANG Daozong

JIAO Shiyi

ZOU Ling

ZHANG Xiaomeng

QIAN Tingting

GAO Zhi

Related Institution

Graduate School of Anhui University of CM

Second Affiliated Hospital of Anhui University of CM

Graduate School, Guangxi University of CM

Department of Acupuncture and Moxibustion, First Affiliated Hospital of Guangxi University of CM

Guangxi Key Laboratory of Molecular Medicine in Prevention and Treatment of Major Diseases with TCM

⁰

Effects of Tongdu Tiaoshen acupuncture on depression-like behavior and Endophilin A1/ROS pathway in hippocampal tissue of CUMS model rats

DOI：10.13703/j.0255-2930.20240922-k0001

摘要

Abstract

关键词

Keywords

references