Effect of <italic style="font-style: italic">Xingnao Kaiqiao</italic> acupuncture therapy on ischemic stroke in rats based on Zn<sup>2+</sup>-mediated TRPA1 channel

LI Honglei; ZHAO Ruyu; XU Wentao

doi:10.13703/j.0255-2930.20241111-k0001

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Effect of Xingnao Kaiqiao acupuncture therapy on ischemic stroke in rats based on Zn²⁺-mediated TRPA1 channel

Study on Mechanism | 更新时间：2026-01-16

- Effect of Xingnao Kaiqiao acupuncture therapy on ischemic stroke in rats based on Zn²⁺-mediated TRPA1 channel
- Chinese Acupuncture & Moxibustion Vol. 46, Issue 1, Pages: 57-65(2026)
- 作者机构：
  
  南京中医药大学针灸推拿学院·养生康复学院，江苏南京　210023
- 作者简介：
- 基金信息：
- DOI：10.13703/j.0255-2930.20241111-k0001
  CLC：
- Received：11 November 2024，
  
  Online First：21 October 2025，
  
  Published：12 January 2026
- 稿件说明：
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LI Honglei, ZHAO Ruyu, XU Wentao. Effect of Xingnao Kaiqiao acupuncture therapy on ischemic stroke in rats based on Zn²⁺-mediated TRPA1 channel[J]. Chinese Acupuncture & Moxibustion, 2026, 46(1): 57-65. DOI: 10.13703/j.0255-2930.20241111-k0001.
DOI：

LI Honglei, ZHAO Ruyu, XU Wentao. Effect of Xingnao Kaiqiao acupuncture therapy on ischemic stroke in rats based on Zn²⁺-mediated TRPA1 channel[J]. Chinese Acupuncture & Moxibustion, 2026, 46(1): 57-65. DOI: 10.13703/j.0255-2930.20241111-k0001. DOI：

摘要

目的：

观察“醒脑开窍”针刺法对缺血性脑卒中大鼠局部血管舒张的影响，探讨针刺对缺血性脑损伤保护作用的机制。

方法：

将80只SPF级雄性SD大鼠随机分为假手术组、模型组、针刺组、锌螯合剂组，每组 20 只。除假手术组外，其余各组大鼠均采用改良的Longa线栓法建立大脑中动脉栓塞模型；假手术组仅进行钝性分离，不插入线栓。针刺组行“醒脑开窍”针刺法干预，穴取双侧“内关”及“水沟”，留针30 min，每日1次，连续干预7 d。锌螯合剂组于造模前30 min腹腔注射锌螯合剂TPEN（15 mg/kg）。于造模后2 h及造模后7 d评定神经行为学评分；于造模前、造模后2 h及造模后7 d采用激光散斑测量大鼠右侧大脑皮层缺血区血管管径，计算血管管径扩缩比；造模后7 d，采用2，3，5-氯化三苯基四氮唑（TTC）染色法检测大鼠脑梗死体积百分比，FLOUZin-3荧光探针检测大鼠右侧大脑皮层缺血区锌离子（Zn

）水平，Western blot 法和实时荧光定量PCR 法检测大鼠右侧大脑皮层缺血区血管舒张相关因子瞬时受体电位锚蛋白1（TRPA1）、降钙素基因相关肽（CGRP）蛋白和mRNA表达，钙测试盒检测大鼠右侧大脑皮层缺血区钙离子（Ca

）含量。

结果：

造模后7 d，与假手术组比较，模型组大鼠神经行为学评分、脑梗死体积百分比、Zn

水平和Ca

含量均升高（

0.05），血管管径扩缩比差异无统计学意义（

0.05），TRPA1、CGRP蛋白和mRNA表达降低（

0.05）。与模型组比较，针刺组与锌螯合剂组大鼠神经行为学评分、脑梗死体积百分比、Zn

水平和Ca

含量降低（

0.05），血管管径扩缩比升高（

0.05），TRPA1、CGRP蛋白和mRNA表达升高（

0.05）。与锌螯合剂组比较，针刺组大鼠神经行为学评分、脑梗死体积百分比升高（

0.05），血管管径扩缩比、Zn

水平、TRPA1蛋白和mRNA表达、CGRP蛋白和mRNA表达、Ca

含量差异无统计学意义（

0.05）。

结论：

“醒脑开窍”针刺法可有效舒张脑血管以减轻缺血性脑损伤，其机制可能与降低游离Zn

水平，激活TRPA1通道促进Ca

内流，从而释放CGRP诱导脑血管舒张有关。

Abstract

Objective

To observe the effect of

Xingnao Kaiqiao

(regaining consciousness and opening orifices) acupuncture therapy on the local vasodilatation of ischemic stroke rats

so as to elucidate the mechanism of the protective effect of acupuncture on ischemic brain injury.

Methods

Eighty SPF-grade male SD rats were randomly divided into a sham-operation group

a model group

an acupuncture group and a zinc chelator (TPEN) group

with 20 rats in each group. Except in the sham-operation group

modified Longa filament method was used to establish rat models of middle cerebral artery occlusion in the other groups. In the sham-o

peration group

the blunt dissection was performed

without the suture occluded. In the acupuncture group

Xingnao Kaiqiao

acupuncture therapy was operated at bilateral "Neiguan" (PC6) and "Shuigou" (GV26)

and the needles were retained for 30 min

once daily

for consecutively 7 days. In the TPEN group

TPEN was injected intraperitoneally (15 mg/kg)

30 min before modeling. The neurobehavioral scores were assessed 2 h and 7 d after modeling respectively. Before modeling

and 2 h and 7 d after modeling

the diameter of blood vessels in the ischemic area of the right cerebral cortex of rats was measured by laser speckle

and the vascular expansion and contraction ratio was calculated. Seven days after modeling

using 2

5-triphenyltetrazolium chloride (TTC) staining method

the percentage of cerebral infarction volume was determined in the ischemic area of the right cerebral cortex of rats

FLOUZin-3 fluorescent probe was used to detect the level of Zn

Western blotting and real-time quantitative PCR were adopted to detect the protein and mRNA expression of the vasodilation-related transient receptor potential factor 1 (TRPA1) and calcitonin gene-related peptide (CGRP)

and calcium test kit was used to detect the content of calcium ion (Ca

) in the ischemic area of the right cerebral cortex of rats.

Results

Seven days after modeling

compared with the sham-operation group

the neurobehavioral score

the percentage of cerebral infarction volume

the level of Zn

and the contents of Ca

increased (

0.05)

vascular expansion and contraction ratio was not different statistically (

0.05)

and the mRNA and protein expression of TRPA1 and CGRP decreased (

0.05) in the model group. Compared with the model group

the neurobehavioral scores

the percentage of cerebral infarction volume

levels and Ca

contents were reduced (

0.05)

vascular expansion and contraction ratio rose (

0.05)

and the mRNA and protein expression of TRPA1 and CGRP was elevated (

0.05) in the acupuncture group and the TPEN group. When compared with the TPEN group

the neurobehavioral score and the percentage of cerebral infarction volume were elevated (

0.05)

and the differences were not significant statistically in Zn

level and Ca

content

vascular expansion and contraction ratio

and the mRNA and protein expression of TRPA1 and CGRP in the acupuncture group (

0.05).

Conclusion

Xingnao Kaiqiao

acupuncture therapy can effectively dilate cerebral blood vessels to alleviate ischemic brain injury

which may be related to reducing the level of free Zn

activating TRPA1 channel to promote Ca

influx

thereby releasing CGRP to induce cerebral vasodilation.

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Related Institution

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⁰

Effect of Xingnao Kaiqiao acupuncture therapy on ischemic stroke in rats based on Zn2+-mediated TRPA1 channel

DOI：10.13703/j.0255-2930.20241111-k0001

摘要

Abstract

关键词

Keywords

references

Effect of Xingnao Kaiqiao acupuncture therapy on ischemic stroke in rats based on Zn²⁺-mediated TRPA1 channel