Effect of moxibustion on serum inflammatory response in rat models of rheumatoid arthritis based on GSDMD

XU Yalin; WANG Haoyu; YANG Xingchun; XU Yanqiu; ZHOU Haiyan; WU Xiao

doi:10.13703/j.0255-2930.20250307-k0005

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Effect of moxibustion on serum inflammatory response in rat models of rheumatoid arthritis based on GSDMD

Study on Mechanism | 更新时间：2026-07-09

- Effect of moxibustion on serum inflammatory response in rat models of rheumatoid arthritis based on GSDMD
- Chinese Acupuncture & Moxibustion Vol. 46, Issue 7, Pages: 1156-1160(2026)
- 作者机构：
  
  西南医科大学附属中医医院治未病中心，四川泸州　646000
  成都中医药大学针灸推拿学院
- 作者简介：
- 基金信息：
- DOI：10.13703/j.0255-2930.20250307-k0005
  CLC：
- Received：07 March 2025，
  
  Online First：08 June 2026，
  
  Published：12 July 2026
- 稿件说明：
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XU Yalin, WANG Haoyu, YANG Xingchun, et al. Effect of moxibustion on serum inflammatory response in rat models of rheumatoid arthritis based on GSDMD[J]. Chinese Acupuncture & Moxibustion, 2026, 46(7): 1156-1160.
DOI：

XU Yalin, WANG Haoyu, YANG Xingchun, et al. Effect of moxibustion on serum inflammatory response in rat models of rheumatoid arthritis based on GSDMD[J]. Chinese Acupuncture & Moxibustion, 2026, 46(7): 1156-1160. DOI： 10.13703/j.0255-2930.20250307-k0005.

摘要

目的：

观察艾灸对类风湿关节炎（RA）大鼠足部外观及血清炎症因子白细胞介素-1β（IL-1β）、白细胞介素-18（IL-18）、基质金属蛋白酶-3（MMP-3）的影响，探索艾灸对消皮素D（GSDMD）介导的细胞焦亡的调控机制。

方法：

将50只SD大鼠随机分为对照组、模型组、艾灸组、GSDMD过表达组、GSDMD过表达联合艾灸治疗组（GSDMD过表达艾灸组），每组10只。除对照组外，其余各组采用弗氏完全佐剂（FCA）建立RA大鼠模型。GSDMD过表达组和GSDMD过表达艾灸组大鼠采用GSDMD慢病毒载体构建过表达状态。艾灸组与GSDMD过表达艾灸组大鼠行艾灸干预，穴取“肾俞”“足三里”，两侧交替施灸，每次每穴5壮，每日1次，连续6 d休息1 d，共干预18次。观察大鼠一般情况，于造模前、造模后7 d、慢病毒注射后7 d、艾灸干预结束后测量大鼠右侧足容积。干预结束后，采用ELISA法检测大鼠血清炎症因子IL-1β、IL-18、MMP-3含量。

结果：

与对照组比较，模型组大鼠右侧足容积增加（

0.05），血清炎症因子IL-1β、IL-18、MMP-3含量升高（

0.05）。与模型组比较，艾灸组大鼠右侧足容积降低（

0.05），血清IL-1β、IL-18、MMP-3含量均降低（

0.05）；GSDMD过表达组大鼠右侧足容积增加（

0.05），血清IL-1β、IL-18、MMP-3含量升高（

0.05）。艾灸组右侧足容积及血清IL-1β、IL-18、MMP-3含量均低于GSDMD过表达艾

灸组（

0.05）。

结论：

艾灸可减轻RA大鼠足部肿胀及血清炎症反应，其可能通过调控GSDMD介导的细胞焦亡实现。

Abstract

Objective

To observe the effects of moxibustion on paw appearance and serum levels of interleukin-1β (IL-1β)

interleukin-18 (IL-18) and matrix metalloproteinase-3 (MMP-3) in model rats of rheumatoid arthritis (RA)

and to explore the regulatory mechanism of moxibustion on gasdermin D (GSDMD)-mediated cell pyroptosis.

Methods

Fifty SD rats were randomly divided into 5 groups

with 10 rats in each group

including a control group

a model group

a moxibustion group

a GSDMD overexpression group

and a GSDMD overexpression combined with moxibustion treatment group (GSDMD overexpression + moxibustion group). Except for the control group

RA rat models were established in the other groups using Freund's complete adjuvant (FCA). In the GSDMD overexpression group and the GSDMD overexpression + moxibustion group

the rats received a GSDMD lentiviral vector to induce GSDMD overexpression. In the moxibustion group and the GSDMD overexpression + moxibustion group

moxibustion was performed at "Shenshu" (BL23) and "Zusanli" (ST36) alternately on both sides

with 5 moxa cones at each point and in each session

for 6 consecutive days followed by 1 day of rest. The intervention completion covered 18 sessions. The general conditions of rats and the changes in right paw volume were observed before modeling

7 days after modeling

7 days after lentiviral injection and after intervention completion. After intervention completion

using ELISA

serum levels of IL-1β

IL-18

and MMP-3 were measured.

Results

Compared with the control group

the model group showed the increase in the right paw volume (

0.05) and serum levels of IL-1β

IL-18 and MMP-3 (

0.05). Compared with the model group

the moxibustion group exhibited the de

crease in the right paw volume (

0.05) and serum levels of IL-1β

IL-18 and MMP-3 (

0.05); and in the GSDMD overexpression group

the volume of right paw was higher (

0.05) and the serum levels of IL-1β

IL-18 and MMP-3 were elevated (

0.05). In the moxibustion group

the volume of right paw and the serum levels of IL-1β

IL-18 and MMP-3 were all lower when compared with the GSDMD overexpression + moxibustion group (

0.05).

Conclusion

Moxibustion can alleviate paw swelling and mitigate the serum inflammatory response in RA rats

which may be obtained by regulating GSDMD-mediated pyroptosis.

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