Effect of moxibustion-induced <italic style="font-style: italic">deqi</italic> on hippocampal autophagy function in APPswe/PSEN1dE9 transgenic Alzheimer′s disease mice

LV Zhimai; XIE Dingyi; LI Haiyan; CHEN Cuiyin; CHEN Rixin

doi:10.13703/j.0255-2930.20250408-k0003

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Effect of moxibustion-induced deqi on hippocampal autophagy function in APPswe/PSEN1dE9 transgenic Alzheimer′s disease mice

Study on Mechanism | 更新时间：2026-09-15

- Effect of moxibustion-induced deqi on hippocampal autophagy function in APPswe/PSEN1dE9 transgenic Alzheimer′s disease mice
- Chinese Acupuncture & Moxibustion Vol. 46, Issue 9, Pages: 1465-1472(2026)
- 作者机构：
  
  江西中医药大学，南昌　330006
  赣南医科大学第一附属医院神经内科，江西　赣州　341000
  江西中医药大学附属医院针灸科
  江西中医药大学热敏灸转化医学研究中心
- 作者简介：
- 基金信息：
- DOI：10.13703/j.0255-2930.20250408-k0003
  CLC：
- Received：08 April 2025，
  
  Online First：21 May 2026，
  
  Published：12 September 2026
- 稿件说明：
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LV Zhimai, XIE Dingyi, LI Haiyan, et al. Effect of moxibustion-induced deqi on hippocampal autophagy function in APPswe/PSEN1dE9 transgenic Alzheimer′s disease mice[J]. Chinese Acupuncture & Moxibustion, 2026, 46(9): 1465-1472. DOI: 10.13703/j.0255-2930.20250408-k0003.
DOI：

LV Zhimai, XIE Dingyi, LI Haiyan, et al. Effect of moxibustion-induced deqi on hippocampal autophagy function in APPswe/PSEN1dE9 transgenic Alzheimer′s disease mice[J]. Chinese Acupuncture & Moxibustion, 2026, 46(9): 1465-1472. DOI: 10.13703/j.0255-2930.20250408-k0003. DOI：

摘要

目的：

探讨艾灸“得气”与非“得气”状态下，对APPswe/PSEN1dE9转基因阿尔茨海默病（AD）模型小鼠自噬调控的影响。

方法：

将40只SPF级雄性APPswe/PSEN1dE9转基因小鼠按1∶3的比例随机分为模型组（

=10）、艾灸组（

=30），10只SPF级雄性C57BL/6J小鼠作为对照组。艾灸组小鼠距“大椎”穴约3 cm进行温和灸，每次40 min，每日1次，共干预28 d，根据艾灸干预的前7 d内尾温变化分为艾灸“得气”组[平均尾温升高（TTI）

1 ℃，

=20

]

和艾灸非“得气”组（平均TTI≤1 ℃，

=10）。干预后，各组小鼠行Morris水迷宫实验检测认知行为；HE染色法观察小鼠海马组织形态学；透射电镜观察小鼠海马自噬相关超微结构；Western blot法检测小鼠海马β淀粉样蛋白（Aβ）及自噬相关蛋白[微管相关蛋白轻链3（LC3）Ⅱ、LC3Ⅰ、苄氯素-1（Beclin-1）、泛素结合蛋白（p62）、溶酶体相关膜蛋白1（LAMP1）

]

表达水平。

结果：

与对照组比较，模型组连续5 d的隐蔽平台实验中逃避潜伏期延长（

0.001），空间探索实验平台穿越次数

减少（

0.000 1）；海马神经元排列紊乱，细胞形态异常，自噬-溶酶体复合物数量较少，内部可见大量未溶解的细胞成分；海马LC3Ⅱ/LC3Ⅰ比值及Aβ、p62、LAMP1蛋白表达升高（

0.001，

0.000 1，

0.05），Beclin-1蛋白表达降低（

0.000 1）。与模型组比较，艾灸非“得气”组后2 d逃避潜伏期缩短（

0.05），艾灸“得气”组连续5 d的逃避潜伏期均缩短（

0.05，

0.01）；艾灸非“得气”组、艾灸“得气”组小鼠海马神经元形态改善，可见大量体积增大的自噬小体，溶酶体数量亦明显增加；艾灸非“得气”组、艾灸“得气”组海马Aβ蛋白表达降低（

0.01，

0.001），艾灸“得气”组海马Beclin-1蛋白表达升高（

0.01），p62蛋白表达降低（

0.01）。与艾灸非“得气”组比较，艾灸“得气”组后3 d逃避潜伏期缩短（

0.05）；自噬-溶酶体复合物明显增加，且其内部未溶解的细胞成分明显减少；海马Beclin-1蛋白表达升高（

0.05），p62蛋白表达降低（

0.05），其他指标差异无统计学意义（

0.05）。

结论：

艾灸“得气”较非“得气”能明显改善AD模型小鼠的自噬功能及认知行为学表现。

Abstract

Objective

To investigate the effect of moxibustion on autophagy regulation under

deqi

(arrival of

) versus non-

deqi

moxibustion

using APPswe/PSEN1dE9 transgenic Alzheimer′s disease (AD) model mice.

Methods

Forty SPF-grade male APPswe/PSEN1dE9 transgenic mice were randomly divided into a model group (

=10) and a moxibustion group (

=30) at a ratio of 1∶3. Ten SPF-grade male C57BL/6 mice served as the control group. In the moxibustion group

gentle moxibustion was operated at the site around 3 cm far from "Dazhui" (GV14)

40 min each intervention

once daily and for 28 consecutive days. The deqi group (the mean tail temperature increase [TTI

]

1 ℃ within 7 days

=20) and non-deqi group (the mean TTI≤1 ℃

=10) were subdivided according to the change of tail temperature within 7 days after intervention with moxibustion. After intervention completion

Morris water maze was performed to determi

ne cognitive behavior of mice

HE staining was to observe hippocampal morphology

transmission electron microscopy (TEM) was to observe hippocampal autophagy-related ultrastructure; Western blot analysis was to detect the expression of hippocampal β-amyloid protein (Aβ) and autophagy-related proteins (microtubule-associated protein light chain 3 [LC3

]

Ⅱ、LC3Ⅰ

benzochlorine 1 [Beclin-1

]

ubiquitin-binding protein [p62

]

lysosome-associated membrane protein 1 [LAMP1

]

Results

Compared with the control group

in the model group

the escape latency was prolonged in the hidden platform test for 5 consecutive days (

0.001)

and the number of platform crossings was reduced in spatial probe test (

0.000 1). The hippocampal neuronal arrangement was disorganized

cell morphology was abnormal

and the number of autophagolysosome complexes reduced

and a large number of insoluble cellular components were visible within them. The ratio of LC3Ⅱ/LC3Ⅰand the protein expression of Aβ

p62 and LAMP1 increased (

0.001

0.000 1

0.05)

while the protein expression of Beclin-1 decreased (

0.000 1). When compared with the model group

the escape latency was shortened during the last 2 days of hidden platform test in the non-deqi group (

0.05)

and it was shortened for 5 consecutive days in the deqi group (

0.05

0.01); in the deqi group and the non-deqi group

the hippocampal neuronal morphology was improved

a large number of autophagosomes increased in size

and the number of lysosomes rose. Besides

the hippocampal protein expression of A β was reduced in both the deqi group and the non-deqi group (

0.01

0.001)

the hippocampal protein expression of Beclin-1 was elevated (

0.01) and that of p62 was reduced (

0.01) in the deqi gro

up. When compared with the non-deqi group

in the deqi group

the escape latency was shortened during the last 3 days of the hidden platform test(

0.05)

the number of autophagolysosome complexes increased markedly

the amount of insoluble cellular components inside was significantly reduced; the protein expression of hippocampal Beclin-1 increased (

0.05) and that of p62 decreased (

0.05). The differences in the other indexes were not significant statistically between the two groups (

0.05).

Conclusion

Moxibustion with

deqi

obviously improves autophagic function and cognitive behavioral performance in AD model

versus moxibustion without

deqi

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⁰

Effect of moxibustion-induced deqi on hippocampal autophagy function in APPswe/PSEN1dE9 transgenic Alzheimer′s disease mice

DOI：10.13703/j.0255-2930.20250408-k0003

摘要

Abstract

关键词

Keywords

references