Neural mechanism of acupuncture-driven vagus nerve-gastric reflex regulating gastric motility in mice with diabetic gastroparesis

LIU Yueyue; LIU Yun; WANG Ying; SUN Hang; ZHU Bing; GAO Xinyan

doi:10.13703/j.0255-2930.20251124-k0001

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Neural mechanism of acupuncture-driven vagus nerve-gastric reflex regulating gastric motility in mice with diabetic gastroparesis

Study on Mechanism | 更新时间：2026-05-12

- Neural mechanism of acupuncture-driven vagus nerve-gastric reflex regulating gastric motility in mice with diabetic gastroparesis
- Chinese Acupuncture & Moxibustion Vol. 46, Issue 5, Pages: 773-781(2026)
- 作者机构：
  
  中国中医科学院针灸研究所，北京　100700
  长春中医药大学东北亚中医药研究院
- 作者简介：
- 基金信息：
- DOI：10.13703/j.0255-2930.20251124-k0001
  CLC：
- Received：24 November 2025，
  
  Online First：19 March 2026，
  
  Published：12 May 2026
- 稿件说明：
移动端阅览
LIU Yueyue, LIU Yun, WANG Ying, et al. Neural mechanism of acupuncture-driven vagus nerve-gastric reflex regulating gastric motility in mice with diabetic gastroparesis[J]. Chinese Acupuncture & Moxibustion, 2026, 46(5): 773-781.
DOI：

LIU Yueyue, LIU Yun, WANG Ying, et al. Neural mechanism of acupuncture-driven vagus nerve-gastric reflex regulating gastric motility in mice with diabetic gastroparesis[J]. Chinese Acupuncture & Moxibustion, 2026, 46(5): 773-781. DOI： 10.13703/j.0255-2930.20251124-k0001.

摘要

目的：

观察电针经迷走神经对糖尿病性胃轻瘫（DGP）小鼠胃运动的即刻调控作用，探讨穴位产生作用可能的迷走神经相关中枢机制。

方法：

选取清洁级雄性C57BL/6小鼠27只，将其中12只小鼠随机分为对照1组、模型1组，每组6只，给予电针“足三里”“内关”“中脘”并检测胃内压相关指标，离断小鼠膈下迷走神经，再次给予上述穴位相同电刺激并检测胃内压相关指标；剩余15只小鼠随机分为对照2组、模型2组、足三里组、内关组、中脘组，每组3只，足三里组、内关组、中脘组分别予电针相应穴位，采用免疫荧光染色法观察小鼠迷走神经运动背核（DMV）中胆碱乙酰转移酶（ChAT）神经元激活数量。除对照1组和对照2组外，余组采用腹腔注射链脲佐菌素（120 mg/kg）联合高脂饲料喂养建立糖尿病模型，8周后检测小鼠胃排空率验证DGP造模情况；电针干预均采用连续波，频率10 Hz，电流1 mA。

结果：

与对照1组比较，模型1组小鼠空腹血糖升高（

0.001），造模第8周体质量升高（

0.01），胃排空率降低（

0.001）。与膈下迷走神经离断术前（pre-sVX）基线比较，对照1组、模型1组小鼠膈下迷走神经离断术后（post-sVX）胃运动频率下降（

0.001，

0.05）；与pre-sVX比较，对照1组、模型1组post-sVX电针“足三里”“内关”胃运动振幅、曲线下面积、频率均下降（

0.001）；与pre-sVX电针“内关”比较，对照1组、模型1组pre-sVX电针“足三里”胃运动振幅、曲线下面

积均升高（

0.001）。与对照2组比较，模型2组小鼠DMV中ChAT神经元激活数量减少（

0.001）；与模型2组比较，足三里组、内关组小鼠DMV中ChAT神经元激活数量增加（

0.001，

0.05）；与内关组比较，足三里组小鼠DMV中ChAT神经元激活数量增加（

0.01）。

结论：

电针“足三里”“内关”均能促进DGP小鼠胃运动，“足三里”较“内关”促进胃运动的作用更显著，其神经机制可能与DMV中ChAT神经元下行经迷走神经调控胃动力相关。

Abstract

Objective

To observe the immediate regulatory effect of electroacupuncture (EA) on gastric motility via the vagus nerve in mice with diabetic gastroparesis (DGP)

and to explore the potential central mechanism related to vagus nerve underlying the effects of acupoints.

Methods

A total of 27 male C57BL/6 mice of clean grade were selected. Of them

12 mice were randomly divided into a control group 1 and a model group 1

6 mice in each group. In the two groups

EA was applied at "Zusanli" (ST36)

"Neiguan" (PC6) and "Zhongwan" (CV12)

and the intragastric pressure-related indexes were detected. After subdiaphragmatic vagus nerve transection (sVX)

the same EA stimulation was delivered

and the intragastric pressure-related indexes were detected again in the two groups. The remaining 15 mice were randomly divided into a control group 2

a model group 2

a Zusanli group

a Neiguan group and a Zhongwan group

3 mice in each group. In the Zusanli group

the Zhongwan group and the Neiguan group

EA was applied at "Zusanli" (ST36)

"Zhongwan" (CV12) and "Neiguan" (PC6)

respectively

the number of activated choline acetyltransferase (ChAT) neurons in the dorsal motor nucleus of the vagus nerve (DMV) was detected by immunofluorescence staining. Except the control groups 1 and 2

in the remaining groups

diabetes model was established by intraperitoneal injection of streptozotocin (120 mg/kg) combined with high-fat diet feeding. Eight weeks into modeling

the gastric emptying rate was measured to verify the DGP model establishment. Continuous wave was adopted for all EA interventions

of a frequency of 10 Hz and a current of 1 mA.

Results

Compared with the control group 1

the fasting blood glucose was increased (

0.001)

the body weight was increased 8 weeks into modeling (

0.01)

the gastric emptying rate was decreased (

0.001) in the model group 1. Compared with the baseline before sVX (pre-sVX)

the gastric motility frequency after sVX (post-sVX) was decreased in the control group 1 and the model group 1 (

0.001

0.05). Compared with pre-sVX

the gastric motility amplitude

area under the curve (AUC) and frequency post-sVX of EA at "Zusanli" (ST36) and "Neiguan" (PC6) were decreased in the control group 1 and the model group 1 (

0.001). Compared with EA at "Neiguan" (PC6) pre-sVX

the gastric motility amplitude and AUC pre-sVX of EA at "Zusanli" (ST36) were increased in the control group 1 and the model group 1 (

0.001). Compared with the control group 2

the number of activated ChAT neurons in the DMV was decreased in the model group 2 (

0.001). Compared with the model group 2

the number of activated ChAT neurons in the DMV was increased in the Zusanli group and the Neiguan group (

0.001

0.05). Compared with the Neiguan group

the number of activated ChAT neurons in the DMV was increased in the Zusanli group (

0.01).

Conclusion

EA at both "Zusanli" (ST36) and "Neiguan" (PC6) can promote gastric motility in DGP mice

and the effect of "Zusanli" (ST36) is superior to that of "Neiguan" (PC6). Its neural mechanism may be related to the regulation of gastric motility by descending ChAT neurons in the DMV through the vagus nerve.

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