Objective To explore the relationship between type Ⅲ chronic prostatitis and the positive reaction of foot three yin-meridians based on meridian diagnosis. Methods Using the traditional meridian diagnosis combined with tenderness meter detection

the positive reaction rate of meridians and acupoints of crural foot three yin-meridians and tenderness pain threshold of standard acupoint location were compared in the type Ⅲ chronic prostatitis patients（prostatitis group

32 cases） and healthy subjects（health group

30 cases）. Results The positive reaction rate of the spleen meridian was higher than those in the kidney meridian and the liver meridian in the prostatitis group（P<0.001）. The positive reaction rates of the spleen meridian

the kidney meridian and the liver meridian and the total positive reaction rate of foot three yin-meridians in the prostatitis group were higher than those in the health group（P<0.001）. In the prostatitis group

the positive reaction rates of Sanyinjiao（SP 6）

Yinlingquan（SP 9）

Taixi（KI 3）

Ligou（LR 5）

Diji（SP 8）

Ququan（LR 8）

Shangqiu（SP 5） and Zhongfeng（LR 4） were higher than those in the health group（P<0.001）

the tenderness pain threshold of acupoints of crural foot three yin-meridians was lower than the health group（P<0.001）. The positive reaction rate of the spleen meridian was positively correlated with the pain score and the total score of National Institute of Health chronic prostatitis symptom index（NIH-CPSI）

and the positive reaction rate of the kidney meridian was positively correlated with age and international prostate symptom score（IPSS） in the prostatitis group. Conclusion The positive reactions of foot three yin-meridians

especially the spleen meridian

are closely related to the pathological state of type Ⅲ chronic prostatitis

pain and urination symptom are significantly correlated with the spleen meridian and the kidney meridian respectively.