穴位注射对甲型流感病毒诱导小鼠肺炎模型的作用机制

刘明江; 韩子逸; 王惠文; 于成龙; 徐霄龙; 秦涛; 李金贵; 刘清泉

doi:10.13703/j.0255-2930.20210713-k0002

您当前的位置：

首页 >

文章列表页 >

穴位注射对甲型流感病毒诱导小鼠肺炎模型的作用机制

机制探讨 | 更新时间：2023-05-10

- 穴位注射对甲型流感病毒诱导小鼠肺炎模型的作用机制
- Effect and mechanism of acupoint injection on influenza A virus induced pneumonia in mice
- 中国针灸 2022年42卷第9期页码：1017-1023
- 作者机构：
  
  1. 扬州大学兽医学院
  2. 江苏省动物重要疫病与人畜共患病防控协同创新中心
  3. 首都医科大学附属北京中医医院
- 作者简介：
- 基金信息：
  
  十三五国家科技重大专项项目：2017ZX10305501;江苏省高校优势学科建设工程资助项目
- DOI：10.13703/j.0255-2930.20210713-k0002
  中图分类号： R245
- 纸质出版：2022
- 稿件说明：
移动端阅览
刘明江, 韩子逸, 王惠文, 等. 穴位注射对甲型流感病毒诱导小鼠肺炎模型的作用机制[J]. 中国针灸, 2022,42(9):1017-1023.

Effect and mechanism of acupoint injection on influenza A virus induced pneumonia in mice[J]. Chinese Acupuncture and Moxibustion, 2022, 42(9): 1017-1023.
刘明江, 韩子逸, 王惠文, 等. 穴位注射对甲型流感病毒诱导小鼠肺炎模型的作用机制[J]. 中国针灸, 2022,42(9):1017-1023. DOI： 10.13703/j.0255-2930.20210713-k0002.

Effect and mechanism of acupoint injection on influenza A virus induced pneumonia in mice[J]. Chinese Acupuncture and Moxibustion, 2022, 42(9): 1017-1023. DOI： 10.13703/j.0255-2930.20210713-k0002.

摘要

目的：探讨“天突”“曲池”“足三里”注射0.1%维生素C(V_C)+复合维生素B(V_BCo)溶液（VC+VBCo）对甲型流感病毒A/PR/8/34株[influenza A virus A/PR/8/34(H1N1)

PR8]诱导小鼠肺炎模型的作用及机制。方法：将60只雄性ICR小鼠随机分为空白组、模型组、穴位注射组、腹腔注射组、非穴组、利巴韦林组，每组10只。除空白组外，其他各组小鼠经鼻孔缓慢滴入50μL PR8病毒液诱导肺炎模型。实验第2天，穴位注射组小鼠分别在“天突”“曲池”（左侧）、“足三里”（左侧）注射40μL的VC+VBCo溶液；腹腔注射组小鼠腹腔注射120μL的V_C+V_BCo溶液；非穴组小鼠分别在“天突”左侧和左侧“曲池”“足三里”外侧平行旁开0.5cm处注射40μL的V_C+V_BCo溶液；利巴韦林组小鼠腹腔注射120μL利巴韦林溶液，均每天1次，连续7 d。进行3次平行实验。统计各组小鼠平均死亡率、存活时间，比较各组小鼠体质量、肺指数，HE染色法观察各组小鼠肺组织形态，实时荧光定量PCR法检测各组小鼠肺组织病毒载量，ELISA法检测各组小鼠肺组织炎性因子[肿瘤坏死因子α(TNF-α)、白介素（IL）-1β、IL-10]含量，化学发光法检测各组小鼠肺组织氧化应激指标[超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、丙二醛（MDA）]含量。结果：与空白组比较，模型组小鼠体质量降低、肺指数升高（P<0.01）；与模型组比较，穴位注射组小鼠体质量升高（P<0.05），穴位注射组、利巴韦林组小鼠肺指数降低（P<0.05）。穴位注射组小鼠平均死亡率降低、平均存活时间延长（P<0.01

P<0.05），利巴韦林组小鼠平均死亡率降低（P<0.05）。模型组小鼠肺泡结构不完整，肺泡间隔增厚，炎性细胞浸润、红细胞渗出严重；与模型组比较，穴位注射组、利巴韦林组小鼠肺泡结构较完整，肺泡间隔增厚减轻，炎性细胞浸润、红细胞渗出明显减少。与模型组比较，利巴韦林组小鼠肺组织病毒载量降低（P<0.01）。与空白组比较，模型组小鼠肺组织TNF-α、IL-1β、MDA含量升高（P<0.01）

IL-10、SOD、GSH-Px含量降低（P<0.01）；与模型组比较，穴位注射组、利巴韦林组小鼠肺组织TNF-α、IL-1β、MDA含量降低（P<0.05

P<0.01）

IL-10、SOD、GSH-Px含量升高（P<0.01

P<0.05）。结论：V_C+V_BCo穴位注射可在对病毒载量无影响的情况下，减轻PR8诱导的肺炎小鼠肺组织炎性反应和氧化应激，提高存活率、延长存活时间。

Abstract

Objective To investigate the effect and mechanism of acupoint injection with 0.1% vitamin C+vitamin B complex solution（V

Co） at "Tiantu"（CV 22）

"Quchi"（LI 11） and "Zusanli"（ST 36） in mouse model of pneumonia induced by influenza A virus（A/PR/8/34 [H1N1]

PR8）.Methods Sixty male ICR mice were randomized into 6 groups

i.e.control group

model group

acupoint injection group

intraperitoneal injection group

non-target point group and ribavirin group

10 mice in each one.Except the control group

the pneumonia models were induced by slow nasal dripping PR8 virus in the other groups.On the 2nd day of experiment

Co solution

40 μL was injected at "Tiantu"（CV 22）

"Quchi"（LI 11

left） and "Zusanli"（ST 36

left） in the acupoint injection group;V

Co solution

120 μL was injected intraperitoneally in the intraperitoneal injection group;V

Co solution

40 μL was injected at non-target acupoints（0.5 cm away from "Tiantu" [CV 22] to the left side

"Quchi" [LI 11

left]

and "Zusanli" [ST 36

left]） in the non-target point group;and ribavirin solution

120 μL was injected intraperitoneally in the ribavirin group.The intervention was delivered once daily

for consecutive 7 days.Three parallel experiments were undertaken.The mean death rate and survival time were assessed in each group

the body mass and lung index were compared among groups.Using HE staining

the morphology of lung tissue was observed;and with real-time fluorescence quantitative PCR

viral load in lung tissue was detected.The concentrations of inflammatory factors（tumor necrosis factor α [TNF-α]

interleukin [IL]-1β

IL-10） were detected in lung tissue of each group using ELISA;and those of oxidative stress markers（superoxide dismutase [SOD]

glutathione peroxidase [GSH-Px]

malondialdehyde [MDA]

） were detected with chemiluminescence method.Results Compared with the control group

the body mass was decreased and lung index was increased in the model group（P

0.01）.In comparison with the model group

body mass was increased in the acupoint injection group（P

0.05）

lung index was reduced in the acupoint injection group the and ribavirin group（P

0.05）;the mean death rate was decreased and the mean survival time prolonged in the mice of the acupoint injection group（P

0.01

0.05）;and the mean death rate was reduced in the mice of the ribavirin group（P

0.05）.In the model group

the alveolar structure was not integral

the alveolar septum was thickened

inflammatory cells were infiltrated and red blood cells exudated seriously（P

0.01）.Compared with the model group

in the acupoint injection group and the ribavirin group

the alveolar structure was integral

the thickened alveolar septum was alleviated;and the infiltration of inflammatory cells and the exudation of red blood cells were reduced remarkably.The viral load was reduced in the mice of the ribavirin group when compared with the model group（P

0.01）.Compared with the control group

the concentrations of TNF-α

IL-1β and MDA in lung tissue were increased and those of IL-10

SOD and GSH-Px were reduced in the model group（P

0.01）.In the acupoint injection group and the ribavirin group

the concentrations of TNF-α

IL-1β and MDA were reduced in lung tissue and those of IL-10

SOD and GSH-Px were increased（P

0.05

0.01） when compared with the model group.Conclusion Acupoint injection with V

Co solution may alleviate inflammatory responses and oxidative stress in lung tissue of the PR8-induced pneumonia mice

improve survival rate and prolong the survival time in the case of no effect of the viral load.

关键词

Keywords

references

浏览量

217

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

电针内关穴对肺叶切除术患者单肺通气时肺功能的影响

艾灸联合西药治疗瘀血痹阻型类风湿关节炎及对血小板活化的影响

羊肠线、PGLA线埋入对健康大鼠“足三里”穴区皮肤肥大细胞、P物质及组胺的影响

小剂量吗啡内关穴注射治疗急性心肌梗死之胸痛

基于“肾虚督损-毒损脉络”理论针药结合治疗肌张力障碍的临证经验