针刺对脑缺血再灌注损伤大鼠HIF-1α/NLRP3炎性信号的影响

蔡筝韵; 张新昌; 刘芙蓉; 黄正; 杨梦凝; 黄珮妍; 张智慧; 倪光夏

doi:10.13703/j.0255-2930.20210912-k0002

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针刺对脑缺血再灌注损伤大鼠HIF-1α/NLRP3炎性信号的影响

机制探讨 | 更新时间：2024-06-03

- 针刺对脑缺血再灌注损伤大鼠HIF-1α/NLRP3炎性信号的影响
- Effect of acupuncture on HIF-1α/NLRP3 inflammatory signaling pathway in the rats with cerebral ischemia-reperfusion injury
- 中国针灸 2023年43卷第9期页码：1056-1061
- 作者机构：
  
  1.南京中医药大学针灸推拿学院·养生康复学院，江苏南京210023
  2.南京中医药大学针药结合教育部重点实验室，江苏南京210023
- 作者简介：
  
  蔡筝韵，南京中医药大学硕士研究生。E-mail：295658006@qq.com
  倪光夏，主任中医师。E-mail：270010@njucm.edu.cn
- 基金信息：
  
  国家自然科学基金资助项目(82074525);江苏省中医药科技发展计划项目(YB2020005);江苏高校护理学优势学科建设工程资助项目(2019YSHL055);江苏省研究生科研创新项目(KYCX21_1695)
- DOI：10.13703/j.0255-2930.20210912-k0002
  中图分类号： R 245.3
- 收稿：2021-09-12，
  
  网络首发：2022-06-26，
  
  纸质出版：2023-09-12
- 稿件说明：
移动端阅览
蔡筝韵,张新昌,刘芙蓉等.针刺对脑缺血再灌注损伤大鼠HIF-1α/NLRP3炎性信号的影响[J].中国针灸,2023,43(09):1056-1061.

CAI Zheng-yun,ZHANG Xin-chang,LIU Fu-rong,et al.Effect of acupuncture on HIF-1α/NLRP3 inflammatory signaling pathway in the rats with cerebral ischemia-reperfusion injury[J].Chinese Acupuncture & Moxibustion,2023,43(09):1056-1061.
蔡筝韵,张新昌,刘芙蓉等.针刺对脑缺血再灌注损伤大鼠HIF-1α/NLRP3炎性信号的影响[J].中国针灸,2023,43(09):1056-1061. DOI： 10.13703/j.0255-2930.20210912-k0002.

CAI Zheng-yun,ZHANG Xin-chang,LIU Fu-rong,et al.Effect of acupuncture on HIF-1α/NLRP3 inflammatory signaling pathway in the rats with cerebral ischemia-reperfusion injury[J].Chinese Acupuncture & Moxibustion,2023,43(09):1056-1061. DOI： 10.13703/j.0255-2930.20210912-k0002.

摘要

目的

观察“醒脑开窍”针法对脑缺血再灌注损伤大鼠缺氧诱导因子1α（HIF-1α）、Nod样受体蛋白3（NLRP3）表达的影响，探讨针刺抗脑缺血再灌注损伤的作用机制。

方法

将72只雄性SD大鼠随机分为假手术组、模型组、针刺组及非穴位针刺组，每组18只。采用改良Longa线栓法建立急性局灶性脑缺血大鼠模型，缺血2 h后进行再灌注建立脑缺血再灌注大鼠模型。再灌注后即刻，针刺组采用“醒脑开窍”针法进行干预，穴取双侧“内关”及“水沟”，非穴位针刺组在非穴点行针刺干预，留针30 min。各组大鼠于再灌注后24 h取材。Zea-Longa神经功能缺损评分法对大鼠脑神经功能损伤程度进行评估，TTC染色法观察大鼠脑梗死体积百分比，HE染色法观察大鼠右侧大脑皮层组织形态，Western blot法检测大鼠右侧大脑皮层HIF-1α、NLRP3蛋白表达情况。

结果

与假手术组比较，模型组大鼠神经功能缺损评分、脑梗死体积百分比升高（

0.01），HIF-1α、NLRP3蛋白表达升高（

0.01）。与模型组比较，针刺组大鼠神经功能缺损评分、脑梗死体积百分比降低（

0.01），HIF-1α、NLRP3蛋白表达下降（

0.01）；非穴位针刺组大鼠以上指标差异无统计学意义（

0.05）。针刺组以上指标均优于非穴位针刺组（

0.01）。与假手术组比较，模型组与非穴位针刺组大鼠脑组织疏松水肿，细胞核皱缩，针刺组大鼠脑组织形态趋近假手术组。

结论

针刺干预可减轻脑缺血再灌注损伤，其作用机制可能与调控损伤侧大脑皮层HIF-1α、NLRP3表达从而减轻炎性反应有关。

Abstract

Objective

To observe the effects of

Xingnao Kaiqiao

(regaining consciousness and opening orifices) acupuncture therapy on the expression of hypoxia-inducible factor 1α (HIF-1α) and Nod-like receptor protein 3 (NLRP3) in cerebral ischemia-reperfusion rats

and to explore the mechanism of acupuncture against cerebral ischemia-reperfusion injury.

Methods

Seventy-two ma

le SD rats were randomly divided into a sham-operation group

a model group

an acupuncture group and a non-point acupuncture group

with 18 rats in each one. Using modified Longa thread embolization method

the rat model of acute focal cerebral ischemia was prepared; and after 2 h ischemia

the reperfusion was performed to prepared the model of cerebral ischemia-reperfusion. Immediately after reperfusion

Xingnao Kaiqiao

acupuncture method was applied to bilateral "Neiguan" (PC 6) and "Shuigou" (GV 26) in the acupuncture group

while in the non-point acupuncture group

acupuncture was delivered at non-points and all of the needles were retained for 30 min in these two groups. The samples were collected 24 h after reperfusion in the rats of each group. Zea-Longa neurological deficit score was used to evaluate the degree of cerebral neurological impairment

TTC staining was adopted to observe the volume percentage of cerebral infarction

HE staining was provided to observe the morphological changes of brain

and Western blot was applied for detecting the expression of HIF-1α and NLRP3 proteins in the cerebral cortex on the right side.

Results

Compared with the sham-operation group

neurological deficit score and volume percentage of cerebral infarction were increased in the model group (

0.01)

and HIF-1α and NLRP3 protein expression was elevated (

0.01). Compared with the model group

neurological deficit score and volume percentage of cerebral infarction were decreased (

0.01)

and HIF-1α and NLRP3 protein expression was lower (

0.01) in the acupuncture group. There was no significant difference in above indexes in the non-point acupuncture group compared with the model group

0.05). Compared with the sham-operation group

the brain tissue of the rats in the model group and the non-point acupuncture group was loose and edema

and the nuclei were shriveled. The

brain tissue morphology in the acupuncture group was similar to that of the sham-operation group.

Conclusion

Acupuncture can alleviate cerebral ischemia-reperfusion injury

and its mechanism may be related to the regulation of HIF-1α/NLRP3 signaling pathway to attenuate inflammatory response.

关键词

Keywords

references

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