电针不同部位腧穴调节大鼠胃运动效应特征研究：响应时间

余芝; 陆梦江; 韩旭; 徐斌

doi:10.13703/j.0255-2930.20220409-0002

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电针不同部位腧穴调节大鼠胃运动效应特征研究：响应时间

机制探讨 | 更新时间：2024-06-03

- 电针不同部位腧穴调节大鼠胃运动效应特征研究：响应时间
- Effect characteristics of electroacupuncture at the acupoints of different regions in regulating gastric motility of the rats: response time
- 中国针灸 2024年44卷第1期页码：73-77
- 作者机构：
  
  南京中医药大学针药结合教育部重点实验室，江苏南京 210023
- 作者简介：
  
  徐斌，研究员。E-mail：xubin@njucm.edu.cn
- 基金信息：
  
  国家自然科学基金资助项目(82074532);81574071、81873238、81673883
- DOI：10.13703/j.0255-2930.20220409-0002
  中图分类号：
- 收稿：2022-04-09，
  
  修回：2023-09-18，
  
  网络首发：2023-10-12，
  
  纸质出版：2024-01-12
- 稿件说明：
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余芝，陆梦江，韩旭，等.电针不同部位腧穴调节大鼠胃运动效应特征研究：响应时间［J］.中国针灸，2024，44（1）：73-77.

YU Zhi,LU Mengjiang,HAN Xu,et al.Effect characteristics of electroacupuncture at the acupoints of different regions in regulating gastric motility of the rats: response time[J].Chinese Acupuncture & Moxibustion,2024,44(01):73-77.
余芝，陆梦江，韩旭，等.电针不同部位腧穴调节大鼠胃运动效应特征研究：响应时间［J］.中国针灸，2024，44（1）：73-77. DOI： 10.13703/j.0255-2930.20220409-0002.

YU Zhi,LU Mengjiang,HAN Xu,et al.Effect characteristics of electroacupuncture at the acupoints of different regions in regulating gastric motility of the rats: response time[J].Chinese Acupuncture & Moxibustion,2024,44(01):73-77. DOI： 10.13703/j.0255-2930.20220409-0002.

摘要

目的

观察分别电针“中脘”和“足三里”单穴对胃运动的影响，探讨不同部位腧穴调节胃运动的响应时间及其神经机制。

方法

将33只SD大鼠随机分为正常组、迷走神经切断组和内脏大神经切断组，每组11只。迷走神经切断组和内脏大神经切断组大鼠分别予胃迷走神经切断术及左膈角与腰肌之间、肾上腺内侧内脏大神经切断术。各组大鼠均依次予电针“中脘”和左侧“足三里”单穴（疏密波，频率2 Hz/15 Hz，电流2 mA），每穴电针1次，每次1 min。采用多道生理信号采集系统记录各组大鼠电针前1 min及电针0~60 s、0~30 s、30~60 s胃内压，并计算胃内压变化倍率以比较各组效应量。

结果

①与干预前比较，正常组大鼠电针“中脘”0~60 s、0~30 s、30~60 s期间胃内压下降（

0.01）；电针“足三里”0~60 s及30~60 s期间胃内压升高（

0.01）。②与干预前比较，电针“中脘”0~60 s、0~30 s和30~60 s期间，迷走神经切断组及内脏大神经切断组大鼠胃内压降低（

0.01，

0.05）；电针“足三里”0~60 s、0~30 s和30~60 s期间

，内脏大神经切断组大鼠胃内压升高（

0.05）。③与正常组比较，迷走神经切断组大鼠电针“中脘”30~60 s期间抑制效应量降低（

0.01）；内脏大神经切断组大鼠电针“中脘”0~30 s及30~60 s期间抑制效应量降低（

0.01）。电针“中脘”0~30 s及30~60 s期间，内脏大神经切断组抑制效应量较迷走神经切断组降低更明显（

0.01）。与正常组比较，迷走神经切断组大鼠电针“足三里”30~60 s期间兴奋效应量降低（

0.01）；内脏大神经切断组大鼠电针“足三里”0~30 s期间兴奋效应量增加（

0.05）。电针“足三里”0~30 s、30~60 s期间内脏大神经切断组大鼠兴奋效应量较迷走神经切断组增加更明显（

0.01）。

结论

电针不同部位腧穴对正常大鼠胃运动调节效应的响应时间特点及神经机制不同。“中脘”响应时间短，30 s内快速起效；“足三里”响应时间长，30 s后起效。电针0~30 s期间，“中脘”和 “足三里”的效应均为内脏大神经支配占优势；30~60 s期间，“中脘”的效应可能受内脏大神经和迷走神经双重支配，“足三里”的效应受迷走神经支配为主。

Abstract

Objective

To observe the effect of electroacupuncture (EA) at "Zhongwan" (CV 12) and "Zusanli" (ST 36) on gastric motility and explore the response time of the acupoints at different regions for regulating gastric motility and its neuromechanism.

Methods

Thirty-three SD rats were randomly divided into a normal group

a vagectomy group and a splanchnicotomy group

11 rats in each one. Vagectomy and splanchnicotomy were operated in the rats of the vagectomy group and the splanchnicotomy group

respectively. In each group

EA was delivered at "Zhongwan" (CV 2) and "Zusanli"(ST 36) on the left successively (with disperse-dense wave

2 Hz/15 Hz in frequency and 2 mA in intensity); and it was performed at one acupoint for 1 min. Using multiple physiological signal collecting system

the intragastric pressure was measured 1 min before EA and in 0 s to 60 s

0 s to 30 s and 30 s to 60 s of EA in the rats of each group

separately. The change rate of intragastric pressure was calculated to compare the effect size among groups.

Results

1) Compared with that before intervention

in the normal group

the intragastric pressure was dropped in 0 s to 60 s

0 s to 30 s and 30 s to 60 s of EA at "Zhongwan" (CV 12) (

0.01); and it was elevated in 0 s to 60 s and 30 s to 60 s of EA at "Zusanli" (ST 36) (

0.01). 2) In 0 s to 60 s

0 s to 30 s and 30 s to 60 s of EA at "Zhongwan" (CV 12）

the intragastric pressure decreased when compared with that before intervention in both the vagectomy group and the splanchnicotomy group (

0.01

0.05); and it increased in 0 s to 60 s

0 s to 30 s and 30 s to 60 s of EA at "Zusanli" (ST 36) in the splanchnicotomy group (

0.05). 3) Compared with the normal group

the inhibition effect size was reduced in 30 s to 60 s of EA at "Zhongwan" (CV 12) in the vagectomy group (

0.01)

and in 0 s to 30 s and 30 s to 60 s of EA at "Zhongwan" (CV 12) in the splanchnicotomy group (

0.01). During EA at "Zhongwan" (CV 12) for 0 s to 30 s and 30 s to 60 s

the inhibition effect size was declined more obviously in the splanchnicotomy group when compared with that in the vagectomy group (

0.01). The excitation effect size was dropped in the vagectomy group when EA at "Zusanli" (ST 36) for 30 s to 60 s compared with the normal group (

0.01)

while it increased during 0 s to 30 s in the splanchnicotomy group (

0.05). Compared with that of the vagectomy group

the excitation effect size increased more remarkably in the splanchnicotomy group when EA at "Zusanli" (ST 36) for 0 s to 30 s and 30 s to 60 s (

0.01).

Conclusion

Electroacupuncture at the acupoints of different regions differ in the response time and neuromechanism for regulating gastric motility of normal rats. The response time at "Zhongwan" (CV 12) is short and the effect of this acupoint works fast within 30 s; while that at "Zusanli" (ST 36) is prolonged and the effect starts 30 s later. In 0 s to 30 s of EA

the effect of "Zhongwan" (CV 12) and "Zusanli" (ST 36) is predominated by the splanchnic nerve; and in 30 s to 60 s

the effect of "Zhongwan" (CV 12) may be co-innervated by the splanchnic nerve and the vagus nerve

and that of "Zusanli" (ST 36) only by the vagus nerve.

关键词

Keywords

references

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