Objective To observe the effect of wheat-grain moxibustion at "Dazhui"（GV 14）

"Zusanli"（ST 36） and "Sanyinjiao"（SP 6） on Wnt/β-catenin signaling pathway in bone marrow cell in mice with bone marrow inhibition

and to explore the possible mechanism of wheat-grain moxibustion in treating bone marrow inhibition. Methods Forty-five SPF male CD1（ICR） mice were randomly divided into a blank group

a model group and a wheat-grain moxibustion group

15 mice in each group. The bone marrow inhibition model was established by intraperitoneal injection of 80 mg/kg of cyclophosphamide（CTX）. The mice in the wheat-grain moxibustion group were treated with wheat-grain moxibustion at "Dazhui"（GV 14）

"Zusanli"（ST 36） and "Sanyinjiao"（SP 6）

3 moxa cones per acupoint

30 s per moxa cone

once a day

for 7 consecutive days. The white blood cell count（WBC） was measured before modeling

before intervention and 3

5 d and 7 d into intervention. After intervention

the general situation of mice was observed; the number of nucleated cells in bone marrow was detected; the serum levels of interleukin-3（IL-3）

interleukin-6（IL-6） and granulocyte macrophage colony stimulating factor（GM-CSF） were measured by ELISA; the protein and mRNA expression of β-catenin

cyclinD1 and C-Myc in bone marrow cells was measured by Western blot and real-time PCR method. Results Compared with the blank group

the mice in the model group showed sluggish reaction

unstable gait

decreased body weight

and the WBC

number of nucleated cells in bone marrow as well as serum levels of IL-3

IL-6

GM-CSF were decreased（P<0.01）

and the protein and mRNA expression of β-catenin

cyclinD1 and C-Myc was decreased（P<0.01）. Compared with the model group

the mice in the wheat-grain moxibustion group showed better general condition

and WBC

the number of nucleated cells in bone marrow as well as serum levels of IL-3

IL-6

GM-CSF were increased（P<0.01

P<0.05）

and the protein and mRNA expression of β-catenin

cyclinD1 and C-Myc was increased（P<0.05）. Conclusion Wheat-grain moxibustion shows therapeutic effect on bone marrow inhibition

and its mechanism may be related to activating Wnt/β-catenin signaling pathway in bone marrow cells

improving bone medullary hematopoiesis microenvironment and promoting bone marrow cell proliferation.