电头针对缺血性脑卒中大鼠缺血皮质区炎性反应及小胶质细胞极化的影响

彭晓云; 袁博; 田甜; 罗文君; 朱玲桂; 张延菊; 李颖; 杜小正; 王金海

doi:10.13703/j.0255-2930.20221124-k0001

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电头针对缺血性脑卒中大鼠缺血皮质区炎性反应及小胶质细胞极化的影响

机制探讨 | 更新时间：2024-06-03

- 电头针对缺血性脑卒中大鼠缺血皮质区炎性反应及小胶质细胞极化的影响
- Effects of electro-scalp acupuncture on inflammatory response and microglial polarization in the ischemic cortex of rats with ischemic stroke
- 中国针灸 2023年43卷第9期页码：1050-1055
- 作者机构：
  
  1.兰州大学第二医院，康复医学科，甘肃兰州 730030
  3.兰州大学第二医院，中医科，甘肃兰州 730030
  2.甘肃中医药大学附属医院针灸临床中心
  4.甘肃中医药大学针灸推拿学院
- 作者简介：
  
  王金海，主任医师、副教授。E-mail：jhwang0101@163.com
- 基金信息：
  
  国家自然科学基金资助项目(81960896);甘肃中医药大学附属医院院内科研及技术创新基金青年项目(gzfy-2019-13)
- DOI：10.13703/j.0255-2930.20221124-k0001
  中图分类号： R 245.9⁺7
- 收稿：2022-11-24，
  
  网络首发：2023-06-27，
  
  纸质出版：2023-09-12
- 稿件说明：
移动端阅览
彭晓云,袁博,田甜等.电头针对缺血性脑卒中大鼠缺血皮质区炎性反应及小胶质细胞极化的影响[J].中国针灸,2023,43(09):1050-1055.

PENG Xiao-yun,YUAN Bo,TIAN Tian,et al.Effects of electro-scalp acupuncture on inflammatory response and microglial polarization in the ischemic cortex of rats with ischemic stroke[J].Chinese Acupuncture & Moxibustion,2023,43(09):1050-1055.
彭晓云,袁博,田甜等.电头针对缺血性脑卒中大鼠缺血皮质区炎性反应及小胶质细胞极化的影响[J].中国针灸,2023,43(09):1050-1055. DOI： 10.13703/j.0255-2930.20221124-k0001.

PENG Xiao-yun,YUAN Bo,TIAN Tian,et al.Effects of electro-scalp acupuncture on inflammatory response and microglial polarization in the ischemic cortex of rats with ischemic stroke[J].Chinese Acupuncture & Moxibustion,2023,43(09):1050-1055. DOI： 10.13703/j.0255-2930.20221124-k0001.

摘要

目的

观察电头针对缺血性脑卒中大鼠缺血皮质区小胶质细胞标志物CD206、CD32及白细胞介素（IL）-6、IL-1β、IL-10表达的影响，探讨电头针缓解缺血性脑卒中炎性损伤的机制。

方法

从60只7周龄雄性SD大鼠中随机选取15只作为假手术组，剩余大鼠采用线栓法复制大脑中动脉栓塞（MCAO）模型，将造模成功大鼠随机分为模型组、VitD

组和电头针组，每组15只。电头针组取双侧“顶颞前斜线”进行电针干预，疏密波，频率2 Hz /100 Hz，电流强度1 mA，每次30 min，每日1次，共7 d。VitD

组给予1，25-二羟维生素D

（1，25-VitD

）溶液（3 ng/100 g）灌胃，每日1次，共7 d。干预前和干预结束后，观察各组大鼠神经功能缺损评分及神经行为学评分。干预结束后，采用2，3，5-氯化三苯基四氮唑（TTC）染色法检测大鼠脑梗死体积；免疫荧光法检测大鼠缺血皮质区CD32和CD206蛋白表达；Western blot法检测大鼠缺血皮质区IL-6、IL-1β、IL-10蛋白表达。

结果

与假手术组比较，模型组神经功能缺损评分和神经行为学评分升高（

0.01），脑梗死体积增加（

0.01），缺血皮质区CD32、IL-6、IL-1β蛋白表达增加（

0.01），缺血皮质区CD206、IL-10蛋白表达减少（

0.01）。与模型组比较，电头针组和VitD

组神经功能缺损评分和神经行为学评分降低（

0.01），脑梗死体积缩小（

0.01），缺血皮质区CD32、IL-6、IL-1β蛋白表达减少（

0.01），缺血皮质区CD206、IL-10蛋白表达增加（

0.01）。电头针组神经功能缺损评分低于VitD

组（

0.05），脑梗死体积大于VitD

组（

0.05），缺血皮质区CD32、CD206、IL-1β、IL-10蛋白表达少于VitD

组（

0.01，

0.05）。

结论

电头针可改善MCAO模型大鼠神经功能，其机制可能与促进小胶质细胞M1型向M2型极化，缓解炎性损伤有关。

Abstract

Objective

To observe the effects of electro-scalp acupuncture （ESA） on the expression of microglial markers CD206 and CD32

as well as interleukin (IL)-6

IL-1β

and IL-10 in the ischemic cortex of rats with ischemic stroke

and to explore the mechanisms of ESA on alleviating inflammatory damage of ischemic stroke.

Methods

Sixty 7-week-old male SD rats were randomly selected

with 15 rats assigned to a sham surgery group. The remaining rats were treated with suture method to establish rat model of middle cerebral artery occlusion (MCAO). The rats with successful model were randomly divided into a model group

a VitD

group

and an ESA group

with 15 rats in each group. In the ESA group

ESA was performed bilaterally at the "top-temporal anterior oblique line" with disperse-dense wave

a frequency of 2 Hz/100 Hz

and an intensity of 1 mA. Each session lasted for 30 min

once daily

for a total of 7 days. The VitD

group were treated with intragastric administration of 1

25-dihydroxyvitamin D

25-VitD

) solution (3 ng/100 g)

once daily for 7 days. The neurological deficit scores and neurobehavioral scores were assessed before and after the intervention. After the intervention

the brain infarct volume was evaluated using 2

5-triphenyltetrazolium chloride (TTC) staining. Immunofluorescence double staining was performed to detect the protein expression of CD32 and CD206 in the ischemic cortex. Western blot analysis was conducted to measure the protein expression of IL-6

IL-1β

and IL-10 in the ischemic cortex.

Results

Compared with the sham surgery group

the model group showed increased neurological deficit scores and neurobehavioral scores (

0.01)

increased brain infarct volume (

0.01)

increased protein expression of CD32

IL-6

and IL-1β in the ischemic cortex (

0.01)

and decreased protein expression of CD206 and IL-1

0 in the ischemic cortex (

0.01). Compared with the model group

both the ESA group and the VitD

group showed decreased neurological deficit scores and neurobehavioral scores (

0.01)

reduced brain infarct volume (

0.01)

decreased protein expression of CD32

IL-6

and IL-1β in the ischemic cortex (

0.01)

and increased protein expression of CD206 and IL-10 in the ischemic cortex (

0.01). Compared with the VitD

group

the ESA group had lower neurological deficit score (

0.05)

larger brain infarct volume (

0.05)

and lower protein expression of CD32

CD206

IL-1β

and IL-10 in the ischemic cortex (

0.01

0.05).

Conclusion

ESA could improve neurological function in MCAO rats

and its mechanism may be related to promoting microglial M1-to-M2 polarization and alleviating inflammatory damage.

关键词

Keywords

references

Jin R , Liu L , Zhang SH , et al . Role of inflammation and its mediators in acute ischemic stroke [J ] . J Cardiovasc Transl Res , 2013 , 6 ( 5 ): 834 - 851 .

王金海 , 张星华 , 杜小正 , 等 . 头针治疗缺血性卒中疗效的Meta分析 [J ] . 中国循证医学杂志 , 2015 , 15 ( 10 ): 1161 - 1167 .

Wang JH , Tian L , Zhang ZC , et al . Scalp-acupuncture for patients with hemiplegic paralysis of acute ischaemic stroke: a randomized controlled clinical trial [J ] . J Tradit Chin Med , 2020 , 40 ( 5 ): 845 - 854 .

王金海 , 杜小正 , 赵敏 , 等 . 头穴透刺对缺血性中风病患者平衡功能影响的临床研究 [J ] . 江西中医学院学报 , 2013 , 25 ( 2 ): 40 - 42 .

王金海 , 赵敏 , 鲍英存 , 等 . 头穴透刺对急性脑梗死患者血清超敏C反应蛋白及炎性反应因子水平的影响 [J ] . 针刺研究 , 2016 , 41 ( 1 ): 80 - 84 .

王金海 , 张婷卓 , 李兴兰 , 等 . 头穴针刺对局灶性脑缺血大鼠海马旁回炎性反应相关细胞因子表达及脑梗死体积的影响 [J ] . 针刺研究 , 2019 , 44 ( 6 ): 405 - 411 .

王金海 , 杜小正 , 张婷卓 , 等 . 头针对局灶性脑缺血大鼠海马旁回炎性细胞因子表达的影响 [J ] . 针刺研究 , 2019 , 44 ( 3 ): 183 - 188 .

Qin C , Zhou LQ , Ma XT , et al . Dual functions of microglia in ischemic stroke [J ] . Neurosci Bull , 2019 , 35 ( 5 ): 921 - 933 .

Thion MS , Ginhoux F , Garel S . Microglia and early brain development: an intimate journey [J ] . Science , 2018 , 362 ( 6411 ): 185 - 189 .

Chhor V , Le Charpentier T , Lebon S , et al . Characterization of phenotype markers and neuronotoxic potential of polarised primary microglia in vitro [J ] . Brain Behav Immun , 2013 , 32 : 70 - 85 .

Hu XM , Li PY , Guo YL , et al . Microglia/macrophage polarization dynamics reveal novel mechanism of injury expansion after focal cerebral ischemia [J ] . Stroke , 2012 , 43 ( 11 ): 3063 - 3070 .

Cherry JD , Olschowka JA , O'Banion MK . Neuroinflammation and M2 microglia: the good, the bad, and the inflamed [J ] . J Neuroinflammation , 2014 , 11 : 98 .

Tang Y , Le WD . Differential roles of M1 and M2 microglia in neurodegenerative diseases [J ] . Mol Neurobiol , 2016 , 53 ( 2 ): 1181 - 1194 .

Perego C , Fumagalli S , De Simoni MG . Temporal pattern of expression and colocalization of microglia/macrophage phenotype markers following brain ischemic injury in mice [J ] . J Neuroinflammation , 2011 , 8 : 174 .

Longa EZ , Weinstein PR , Carlson S , et al . Reversible middle cerebral artery occlusion without craniectomy in rats [J ] . Stroke , 1989 , 20 ( 1 ): 84 - 91 .

李辞蓉 , 华兴邦 , 周浩良 , 等 . 豚鼠针灸穴位图谱的研制 [J ] . 上海针灸杂志 , 1992 , 11 ( 2 ): 28 - 30 .

高峰 , 郭珲 . 1,25-二羟维生素D 3 通过调节性T细胞延缓肾间质纤维化的作用研究 [J ] . 中国医药导报 , 2020 , 17 ( 13 ): 41 - 44 .

Schäbitz WR , Berger C , Kollmar R , et al . Effect of brain-derived neurotrophic factor treatment and forced arm use on functional motor recovery after small cortical ischemia [J ] . Stroke , 2004 , 35 ( 4 ): 992 - 997 .

彭红霞 , 张伟 , 杨怡 , 等 . 针灸治疗急性缺血性脑血管病的临床效果分析 [J ] . 中医临床研究 , 2019 , 11 ( 24 ): 106 - 108 .

王海桥 , 王凡 , 刘建浩 , 等 . 头针治疗中风偏瘫现代流派简析 [J ] . 中国针灸 , 2010 , 30 ( 9 ): 783 - 786 .

Basic Kes V , Simundic AM , Nikolac N , et al . Pro-inflammatory and anti-inflammatory cytokines in acute ischemic stroke and their relation to early neurological deficit and stroke outcome [J ] . Clin Biochem , 2008 , 41 ( 16/17 ): 1330 - 1334 .

Huang J , Xie YC , Sun XF , et al . DAMPs, ageing, and cancer: the 'DAMP hypothesis' [J ] . Ageing Res Rev , 2015 , 24 ( Pt A ): 3 - 16 .

Mo Y , Xu WL , Fu KJ , et al . The dual function of microglial polarization and its treatment targets in ischemic stroke [J ] . Front Neurol , 2022 , 13 : 921705 .

Zhang CJ , Zhu YB , Wang S , et al . Temporal gene expression profiles after focal cerebral ischemia in mice [J ] . Aging Dis , 2018 , 9 ( 2 ): 249 - 261 .

Boontanrart M , Hall SD , Spanier JA , et al . Vitamin D3 alters microglia immune activation by an IL-10 dependent SOCS 3 mechanism [J ] . J Neuroimmunol , 2016 , 292 : 126 - 136 .

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