“标本配穴”艾灸对腹泻型肠易激综合征伴焦虑模型大鼠心率变异性、心房利钠肽的影响

吴帆; 李佳; 王静芝; 王华; 吴松; 卢威

doi:10.13703/j.0255-2930.20221129-k0004

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“标本配穴”艾灸对腹泻型肠易激综合征伴焦虑模型大鼠心率变异性、心房利钠肽的影响

机制探讨 | 更新时间：2024-06-03

- “标本配穴”艾灸对腹泻型肠易激综合征伴焦虑模型大鼠心率变异性、心房利钠肽的影响
- Effects of moxibustion of "biaoben acupoint combination" on heart rate variability, atrial natriuretic peptide in the model rats of IBS-D complicated with anxiety
- 中国针灸 2023年43卷第10期页码：1139-1147
- 作者机构：
  
  1.湖北中医药大学针灸骨伤学院，武汉 430061
  2.湖北中医药大学中医临床学院
- 作者简介：
  
  吴帆，湖北中医药大学博士研究生。E-mail：412872859@qq.com
  王华，教授。E-mail：hwang.tcm@china.com
- 基金信息：
  
  国家自然科学基金面上项目(81973931)
- DOI：10.13703/j.0255-2930.20221129-k0004
  中图分类号： R 224.2
- 收稿：2022-11-29，
  
  网络首发：2023-08-01，
  
  纸质出版：2023-10-12
- 稿件说明：
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吴帆,李佳,王静芝等.“标本配穴”艾灸对腹泻型肠易激综合征伴焦虑模型大鼠心率变异性、心房利钠肽的影响[J].中国针灸,2023,43(10):1139-1147.

WU Fan,LI Jia,WANG Jing-zhi,et al.Effects of moxibustion of "biaobenacupoint combination" on heart rate variability, atrial natriuretic peptide in the model rats of IBS-D complicated with anxiety[J].Chinese Acupuncture & Moxibustion,2023,43(10):1139-1147.
吴帆,李佳,王静芝等.“标本配穴”艾灸对腹泻型肠易激综合征伴焦虑模型大鼠心率变异性、心房利钠肽的影响[J].中国针灸,2023,43(10):1139-1147. DOI： 10.13703/j.0255-2930.20221129-k0004.

WU Fan,LI Jia,WANG Jing-zhi,et al.Effects of moxibustion of "biaobenacupoint combination" on heart rate variability, atrial natriuretic peptide in the model rats of IBS-D complicated with anxiety[J].Chinese Acupuncture & Moxibustion,2023,43(10):1139-1147. DOI： 10.13703/j.0255-2930.20221129-k0004.

摘要

目的

比较“标本配穴”和“常规取穴”艾灸对腹泻型肠易激综合征（IBS-D）伴焦虑模型大鼠心率变异性（HRV）和心肌心房利钠肽（ANP）表达的影响。

方法

从50只健康SPF级3月龄雌性SD大鼠中随机选取8只作为空白组，剩余大鼠制备IBS-D伴焦虑模型，将造模成功的24只大鼠随机分为模型组、常规组和标本组，每组8只。常规组予“天枢”“足三里”“上巨虚”艾灸干预，标本组予“内关”“足三里”“关元”艾灸干预，每次20 min，每天1次，共干预14 d。分别于干预前后比较各组大鼠体质量、粪便含水率；应用腹壁撤退反射（AWR）实验评价内脏高敏感性，高架十字迷宫和明暗穿箱实验评价焦虑状态。干预后，比较各组大鼠HRV，采用透射电镜观察各组大鼠肠黏膜超微结构，Western blot法及免疫荧光法检测大鼠心肌组织ANP表达。

结果

干预前，与空白组比较，模型组、常规组、标本组大鼠体质量、内脏疼痛阈值降低（

0.05），粪便含水率、AWR评分（扩张压力为40、60、80 mm Hg时，1 mm Hg≈0.133 kPa）升高（

0.05），开放臂停留时间、进入开放臂次数、总运动路程（高架十字迷宫）及明箱停留时间、明暗箱穿梭次数、总运动路程（明暗穿箱）均减少（

0.05）。干预后，与空白组比较，模型组大鼠体质量、内脏疼痛阈值、RR间期标准差（SDNN）、连续RR间隔均方差的平方根（RMSSD）降低（

0.05），粪便含水率、AWR评分（扩张压力为40、60、80 mm Hg时）、低频/高频（LF/HF）、ANP表达升高（

0.05），开放臂停留时间、进入开放臂次数、总运动路程（高架十字迷宫）及明箱停留时间、明暗箱穿梭次数、总运动路程（明暗穿箱）减少（

0.05）；与模型组比较，常规组和标本组大鼠体质量、内脏疼痛阈值、SDNN、RMSSD升高（

0.05），粪便含水率、AWR评分（扩张压力为60、80 mm Hg时）、LF/HF、ANP表达降低（

0.05），开放臂停留时间、进入开放臂次数、总运动路程（高架十字迷宫）及明箱停留时间、明暗箱穿梭次数、总运动路程（明暗穿箱）增多（

0.05）；与常规组比较，标本组大鼠体质量、内脏疼痛阈值、SDNN、RMSSD升高（

0.05），粪便含水率、AWR评分（扩张压力为80 mm Hg时）、LF/HF、ANP表达降低（

0.05），开放臂停留时间、进入开放臂次数、总运动路程（高架十字迷宫）及明箱停留时间、明暗箱穿梭次数、总运动路程（明暗穿箱）增多（

0.05）。空白组大鼠肠黏膜上皮细胞形态正常；模型组大鼠肠黏膜上皮紧密连接形态破坏，缝隙连接增宽；常规组大鼠肠黏膜上皮紧密连接不完整；标本组大鼠肠黏膜上皮紧密连接较完整。

结论

“标本配穴”艾灸较“常规取穴”艾灸能更好地改善IBS-D伴焦虑大鼠症状，其机制可能与缓解焦虑样负性情绪、良性调节HRV、稳固IBS-D肠黏膜屏障、下调心肌ANP表达有关。

Abstract

Objective

To compare the effects on the heart rate variability (HRV) and the expression of the atrial natriuretic peptide (ANP) in the model rats of irritable bowel syndrome with predominant diarrhea (IBS-D) rats complicated with anxiety between moxibustion of "biaoben acupoint combination" and that of "conventional acupoint combination".

Methods

Of 50 healthy SPF female SD rats

aged 3 months

8 rats were selected randomly as a blank group

and the rest rats were prepared to be the model of IBS-D complicated with anxiety. Twenty-four rats after successfully modeled were randomized into a model group

a conventional acupoint combination group (convention group) and a biaoben acupoint combination group (biaoben group)

8 rats in each one. In the convention group

moxibustion was delivered at "Tianshu" (ST 25)

"Zusanli"(ST 36) and "Shangjuxu"(ST 37); and in the biaoben group

moxibustion was applied to "Neiguan" (PC 6)

"Zusanli" (ST 36)

and "Guanyuan" (CV 4). One session of moxibustion took 20 min

once daily

for 14 days in total. Before and after intervention

the body mass and fecal moisture content were compared in the rats of each group; using abdominal wall withdrawal reflex

the visceral hypersensitivity was evaluated; with elevated plus maze (EPM) and light-dark box (LDB)

the anxiety conditions were assessed. After intervention

HRV was compared among groups

the ultrastructure of intestinal mucosa was observed under the transmission electron microscope in the rats of each group

and ANP expression in the myocardial tissue was detected using Western blot method and immunofluorescence.

Results

Before the intervention

compared with the blank group

the body mass and visceral pain threshold of rats were reduced in the model group

the convention group and the biaoben group (

0.05)

fecal moisture content and AWR scores (at the dilatatio

n pressure of 40

60 and 80 mm Hg

1 mm Hg ≈ 0.133 kPa) were elevated (

0.05); and time in the open arm

the open arm entry number and the total movement distance (EPM)

the time spent in the light compartment

the number of dark to light transitions and the total transition distance (LDB) were decreased (

0.05). After the intervention

compared with the blank group

in the model group

the body mass

visceral pain threshold

standard diviation of NN intervals (SDNN) and root mean square of successive RR interval differences (RMSSD) were dropped (

0.05)

fecal moisture content

AWR scores (the dilation pressures of 40

60 and 80 mm Hg)

LF/HF and ANP expression were increased (

0.05)

the time in open arm

the open arm entry number and the total movement distance (EPM)

the time spent in the light compartment

the number of dark to light transitions and the total transition distance (LDB) were decreased (

0.05). When compared with the model group

in the convention group and the biaoben group

the body mass

visceral pain threshold

SDNN and RMSSD were increased (

0.05)

fecal moisture content

AWR scores (the dilation pressures of 60 and 80 mm Hg)

LF/HF and ANP expression were dropped (

0.05)

the time in open arm

the open arm entry number and the total movement distance (EPM)

the time spent in the light compartment

the number of dark to light transitions and the total transition distance (LDB) were increased (

0.05). In the biaoben group

compared with the convention group

the body mass

visceral pain threshold

SDNN and RMSSD were elevated (

0.05)

fecal moisture content

AWR score (the dilation pressure of 80 mm Hg)

LF/HF and ANP expression were decreased (

0.05)

the time in open arm

the open arm entry number and the total movement distance (EPM)

the time spent in the light compartment

the num

ber of dark to light transitions and the total transition distance (LDB) were increased (

0.05). The epithelial cells of the intestinal mucosa showed a normal morphology in the blank group

the tight junction of the cells was disrupted and the junction was loose in the model group; the tight junction was imperfect in the convention group

but it was intact in the biaoben group.

Conclusion

Compared with the conventional acupoint combination

moxibustion of biaoben acupoint combination is more effective on the symptoms of IBS-D complicated with anxiety in the model rats. The effect mechanism may be related to attenuating anxiety-like negative emotions

positively regulating HRV

stabilizing IBS-D intestinal mucosal barrier and down-regulating the expression of ANP in myocardium.

关键词

Keywords

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