电针对2型糖尿病大鼠糖脂代谢及股四头肌和脂肪组织ZAG、GLUT4表达的影响

郭彩凤; 李瑞; 宋姗姗; 段浩茹; 张荣媛; 庄舒婷; 郭卫星; 董滢

doi:10.13703/j.0255-2930.20230129-k0001

您当前的位置：

首页 >

文章列表页 >

电针对2型糖尿病大鼠糖脂代谢及股四头肌和脂肪组织ZAG、GLUT4表达的影响

机制探讨1 | 更新时间：2024-06-03

- 电针对2型糖尿病大鼠糖脂代谢及股四头肌和脂肪组织ZAG、GLUT4表达的影响
- Effects of electroacupuncture on the glucose-lipid metabolism and the expression of ZAG and GLUT4 in the femoral quadriceps and adipose tissue in the rats with type 2 diabetes mellitus
- 中国针灸 2023年43卷第12期页码：1425-1430
- 作者机构：
  
  1.北京中医药大学针灸推拿学院，北京102488
  2.北京中医药大学房山医院针灸科
- 作者简介：
  
  李瑞，教授、主任医师。E-mail：tingxuezhai@126.com
- 基金信息：
- DOI：10.13703/j.0255-2930.20230129-k0001
  中图分类号： R 245.9⁺7
- 收稿：2023-01-29，
  
  网络首发：2023-07-20，
  
  纸质出版：2023-12-12
- 稿件说明：
移动端阅览
郭彩凤,李瑞,宋姗姗等.电针对2型糖尿病大鼠糖脂代谢及股四头肌和脂肪组织ZAG、GLUT4表达的影响[J].中国针灸,2023,43(12):1425-1430.

GUO Cai-feng,LI Rui,SONG Shan-shan,et al.Effects of electroacupuncture on the glucose-lipid metabolism and the expression of ZAG and GLUT4 in the femoral quadriceps and adipose tissue in the rats with type 2 diabetes mellitus[J].Chinese Acupuncture & Moxibustion,2023,43(12):1425-1430.
郭彩凤,李瑞,宋姗姗等.电针对2型糖尿病大鼠糖脂代谢及股四头肌和脂肪组织ZAG、GLUT4表达的影响[J].中国针灸,2023,43(12):1425-1430. DOI： 10.13703/j.0255-2930.20230129-k0001.

GUO Cai-feng,LI Rui,SONG Shan-shan,et al.Effects of electroacupuncture on the glucose-lipid metabolism and the expression of ZAG and GLUT4 in the femoral quadriceps and adipose tissue in the rats with type 2 diabetes mellitus[J].Chinese Acupuncture & Moxibustion,2023,43(12):1425-1430. DOI： 10.13703/j.0255-2930.20230129-k0001.

摘要

目的

观察电针“脾俞”“胃脘下俞”“足三里”“三阴交”对2型糖尿病（T2DM）模型大鼠糖脂代谢及股四头肌和脂肪组织锌α2糖蛋白（ZAG）、葡萄糖转运体4（GLUT4）蛋白表达的影响，探讨电针治疗T2DM的作用机制。

方法

12只雄性ZDF大鼠采用Purina #5008高糖高脂饲料喂养4周诱导T2DM模型，造模成功后随机分为模型组和电针组，每组6只；另选取6只同月龄雄性ZL大鼠作为空白组。对电针组大鼠行电针干预，穴取双侧“脾俞”“胃脘下俞”“足三里”“三阴交”，连续波，频率15 Hz，电流强度2 mA，每次20 min，每日1次，每周连续6次，共4周。于造模前、干预前后测量各组大鼠空腹血糖（FBG），干预前后测量大鼠体质量。干预后，采用酶比色法检测血清总胆固醇（TC）、三酰甘油（TG）、游离脂肪酸（FFA）含量；ELISA法检测血清胰岛素（INS）、ZAG含量，计算胰岛素敏感指数（HOMA-ISI）；采用Western blot法检测股四头肌和脂肪组织ZAG、GLUT4蛋白表达。

结果

干预后，与空白组比较，模型组大鼠FBG、体质量及血清TC、TG、FFA、INS含量升高（

0.01），HOMA-ISI降低（

0.01）；血清ZAG含量，股四头肌和脂肪组织ZAG、GLUT4蛋白表达减少（

0.01）。与模型组比较，电针组大鼠FBG、体质量及血清TC、TG、FFA、INS含量降低（

0.01），HOMA-ISI升高（

0.01）；血清ZAG含量，股四头肌和脂肪组织ZAG、GLUT4蛋白表达增加（

0.01，

0.05）。

结论

电针可调节T2DM模型大鼠糖脂代谢，改善胰岛素抵抗，提高胰岛素敏感性，其作用机制与调控骨骼肌与脂肪组织ZAG、GLUT4蛋白表达有关。

Abstract

Objective

To observe the effects on the glucose-lipid metabolism and the expression of zinc-α2-glycoprotein (ZAG) and glucose transporter 4 (GLUT4) in the femoral quadriceps and adipose tissue after electroacupuncture (EA) at "Pishu" (BL 20)

"Weiwanxiashu" (EX-B 3)

"Zusanli" (ST 36) and "Sanyinjiao" (SP 6) in the rats with diabetes mellitus type 2 (T2DM)

so as to explore the effect mechanism of EA in treatment of T2DM.

Methods

Twelve ZDF male rats were fed with high-sugar and high-fat fodder

Purina #5008 for 4 weeks to induce T2DM model. After successfully modeled

the rats were randomly divided into a model group and an EA group

with 6 rats in each one. Additionally

6 ZL male rats of the same months age were collected as the blank group. The rats in the EA group were treated with EA at bilateral "Pishu" (BL 20)

"Weiwanxiashu" (EX-B 3)

"Zusanli" (ST 36) and "Sanyinjiao" (SP 6)

with continuous wave

15 Hz in frequency

and 2 mA in intensity. The electric stimulation lasted 20 min each time. EA was delivered once daily

6 times a week for 4 weeks. Separately

the levels of fasting blood glucose (FBG) was measured before modeling

before and after intervention

and the body mass of each rat was weighted before and after intervention. After intervention

the levels of the total cholesterol (TC)

triacylglycerol (TG) and free fatty acid (FFA) in serum were detected using enzyme colorimetric method; and the levels of the serum insulin (INS) and ZAG were detected by ELISA. Besides

the insulin sensitivity index (HOMA-ISI) was calculated. With Western blot technique adopted

the protein expressions of ZAG and GLUT4 in the femoral quadriceps and adipose tissue were determined.

Results

After intervention

compared with the blank group

the levels of FBG and body mass

and the levels of serum TC

FFA and INS increased (

0.01)

while HOMA-ISI decreased (

0.01); the level of ZAG in the serum and the p

rotein expressions of ZAG and GLUT4 in the femoral quadriceps and adipose tissue dropped (

0.01) in the model group. In the EA group

compared with the model group

the levels of FBG and body mass

and the levels of serum TC

FFA and INS were reduced (

0.01)

and HOMA-ISI increased (

0.01); the level of ZAG in the serum and the protein expressions of ZAG and GLUT4 in the femoral quadriceps and adipose tissue increased (

0.01

0.05).

Conclusion

Electroacupuncture can effectively regulate glucose-lipid metabolism

improve insulin resistance and sensitivity in the rats with T2DM

which is associated with the modulation of ZAG and GLUT4 expression in the skeletal muscle and adipose tissue.

关键词

Keywords

references

Huang XJ , Liu GH , Guo J , et al . The PI3K/AKT pathway in obesity and type 2 diabetes [J ] . Int J Biol Sci , 2018 , 14 ( 11 ): 1483 - 1496 .

Kane JP , Pullinger CR , Goldfine ID , et al . Dyslipidemia and diabetes mellitus: role of lipoprotein species and interrelated pathways of lipid metabolism in diabetes mellitus [J ] . Curr Opin Pharmacol , 2021 , 61 : 21 - 27 .

Cabassi A , Tedeschi S . Zinc-α2-glycoprotein as a marker of fat catabolism in humans [J ] . Curr Opin Clin Nutr Metab Care , 2013 , 16 ( 3 ): 267 - 271 .

Bao Y , Bing C , Hunter L , et al . Zinc-alpha2-glycoprotein, a lipid mobilizing factor, is expressed and secreted by human (SGBS) adipocytes [J ] . FEBS Lett , 2005 , 579 ( 1 ): 41 - 47 .

宋姗姗 , 李瑞 , 曹昺焱 , 等 . 电针对2型糖尿病肥胖大鼠血糖、血脂、瘦素含量的影响 [J ] . 针灸临床杂志 , 2020 , 36 ( 4 ): 87 - 90 .

宋姗姗 , 李瑞 , 曹昺焱 , 等 . 电针调控骨骼肌胰岛素受体底物-1磷酸化改善胰岛素抵抗的机制 [J ] . 中华中医药杂志 , 2021 , 36 ( 3 ): 1389 - 1392 .

段浩茹 , 李瑞 , 宋姗姗 , 等 . 电针调控骨骼肌AdipoR1/AMPK/PPARα通路改善糖尿病肥胖大鼠糖脂代谢紊乱的机制探讨 [J ] . 针刺研究 , 2021 , 46 ( 11 ): 907 - 913 .

Al-Awar A , Kupai K , Veszelka M , et al . Experimental diabetes mellitus in different animal models [J ] . J Diabetes Res , 2016 , 2016 : 9051426 .

李娟娥 , 王磊 , 秦灵灵 , 等 . 自发性2型糖尿病啮齿类动物模型研究概况 [J ] . 中国实验方剂学杂志 , 2010 , 16 ( 6 ): 267 - 271 .

郭义 . 实验针灸学 [M ] . 北京 : 中国中医药出版社 , 2008 .

Lambadiari V , Triantafyllou K , Dimitriadis GD . Insulin action in muscle and adipose tissue in type 2 diabetes: the significance of blood flow [J ] . World J Diabetes , 2015 , 6 ( 4 ): 626 - 633 .

Heinonen I , Nesterov SV , Kemppainen J , et al . Increasing exercise intensity reduces heterogeneity of glucose uptake in human skeletal muscles [J ] . PLoS One , 2012 , 7 ( 12 ): e52191 .

Sylow L , Tokarz VL , Richter EA , et al . The many actions of insulin in skeletal muscle, the paramount tissue determining glycemia [J ] . Cell Metab , 2021 , 33 ( 4 ): 758 - 780 .

Grundy SM . Overnutrition, ectopic lipid and the metabolic syndrome [J ] . J Investig Med , 2016 , 64 ( 6 ): 1082 - 1086 .

El Amrousy D , El-Afify D , Salah S . Insulin resistance, leptin and adiponectin in lean and hypothyroid children and adolescents with obesity [J ] . BMC Pediatr , 2022 , 22 ( 1 ): 245 .

Luo LP , Liu ML . Adipose tissue in control of metabolism [J ] . J Endocrinol , 2016 , 231 ( 3 ): R77 - R99 .

Bing C , Mracek T , Gao D , et al . Zinc-α 2-glycoprotein: an adipokine modulator of body fat mass? [J ] . Int J Obes (Lond) , 2010 , 34 ( 11 ): 1559 - 1565 .

Russell ST , Tisdale MJ . Studies on the anti-obesity activity of zinc-α2-glycoprotein in the rat [J ] . Int J Obes , 2011 , 35 ( 5 ): 658 - 665 .

Hassan MI , Waheed A , Yadav S , et al . Zinc alpha 2-glycoprotein: a multidisciplinary protein [J ] . Mol Cancer Res , 2008 , 6 ( 6 ): 892 - 906 .

Abel ED , Peroni O , Kim JK , et al . Adipose-selective targeting of the GLUT4 gene impairs insulin action in muscle and liver [J ] . Nature , 2001 , 409 ( 6821 ): 729 - 733 .

Jaldin-Fincati JR , Pavarotti M , Frendo-Cumbo S , et al . Update on GLUT4 vesicle traffic: a cornerstone of insulin action [J ] . Trends Endocrinol Metab , 2017 , 28 ( 8 ): 597 - 611 .

戴燕铃 , 陈雪花 , 丁怀利 , 等 . 薯蓣粥对2型糖尿病模型大鼠糖代谢及胰岛功能的影响 [J ] . 中医杂志 , 2021 , 62 ( 18 ): 1628 - 1634 .

郭胜男 , 张莉 , 吴深涛 , 等 . 化浊解毒方对多氯联苯126暴露致大鼠脂肪胰岛素抵抗的机制 [J ] . 中成药 , 2021 , 43 ( 12 ): 3303 - 3309 .

Ceperuelo-Mallafré V , Ejarque M , Duran X , et al . Zinc-α 2-glycoprotein modulates AKT-dependent insulin signaling in human adipocytes by activation of the PP2A phosphatase [J ] . PLoS One , 2015 , 10 ( 6 ): e0129644 .

Lansey MN , Walker NN , Hargett SR , et al . Deletion of Rab GAP AS160 modifies glucose uptake and GLUT4 translocation in primary skeletal muscles and adipocytes and impairs glucose homeostasis [J ] . Am J Physiol Endocrinol Metab , 2012 , 303 ( 10 ): E1273 - E1286 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

电针调控GLP-1R/PKA通路改善2型糖尿病大鼠胰岛素抵抗及肠黏膜屏障损伤的机制研究

电针对腰椎间盘退变模型兔腰段脊髓小胶质细胞活化的影响

电针调控mTOR/SREBP1信号通路促进肥胖小鼠白色脂肪棕色化的机制研究

“标本配穴”电针预处理减轻心肌缺血再灌注损伤大鼠心肌细胞铜死亡的机制研究

穴位埋线治疗早中期膝关节骨关节炎的疗效与成本–效果评价