针刺对自发性高血压大鼠粪便短链脂肪酸及TLR4/MyD88/NF-κB信号通路的影响

丁志敏; 陈婷玉; 张丽红; 金小琴; 刘斌; 冯晓东; 郑婕

doi:10.13703/j.0255-2930.20230710-k0001

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针刺对自发性高血压大鼠粪便短链脂肪酸及TLR4/MyD88/NF-κB信号通路的影响

机制探讨 | 更新时间：2024-06-14

- 针刺对自发性高血压大鼠粪便短链脂肪酸及TLR4/MyD88/NF-κB信号通路的影响
- Effects of acupuncture on fecal short-chain fatty acids and TLR4/MyD88/NF-κB signaling pathway in spontaneously hypertensive rats
- 中国针灸 2024年44卷第6期页码：661-668
- 作者机构：
  
  1.河南中医药大学康复医学院，郑州　450046
  2.河南中医药大学第一附属医院康复中心
- 作者简介：
  
  ☒郑婕，讲师。E-mail：jiezheng1119@163.com
- 基金信息：
  
  河南省高等学校重点科研项目(21A360013);河南省自然科学基金项目(202300410260);河南中医药大学研究生科研创新项目(2021KYCX031);河南省“双一流”创建学科中医学科学研究专项项目(HSRP-DFCTCM-T-9)
- DOI：10.13703/j.0255-2930.20230710-k0001
  中图分类号：
- 收稿：2023-07-10，
  
  网络首发：2023-11-15，
  
  纸质出版：2024-06-12
- 稿件说明：
移动端阅览
丁志敏, 陈婷玉, 张丽红, 等. 针刺对自发性高血压大鼠粪便短链脂肪酸及TLR4/MyD88/NF-κB信号通路的影响[J]. 中国针灸, 2024,44(6):661-668.

DING Zhimin, CHEN Tingyu, ZHANG Lihong, et al. Effects of acupuncture on fecal short-chain fatty acids and TLR4/MyD88/NF-κB signaling pathway in spontaneously hypertensive rats[J]. Chinese Acupuncture & Moxibustion, 2024, 44(6): 661-668.
丁志敏, 陈婷玉, 张丽红, 等. 针刺对自发性高血压大鼠粪便短链脂肪酸及TLR4/MyD88/NF-κB信号通路的影响[J]. 中国针灸, 2024,44(6):661-668. DOI： 10.13703/j.0255-2930.20230710-k0001.

DING Zhimin, CHEN Tingyu, ZHANG Lihong, et al. Effects of acupuncture on fecal short-chain fatty acids and TLR4/MyD88/NF-κB signaling pathway in spontaneously hypertensive rats[J]. Chinese Acupuncture & Moxibustion, 2024, 44(6): 661-668. DOI： 10.13703/j.0255-2930.20230710-k0001.

摘要

目的：

观察针刺对自发性高血压大鼠（SHR）血压、粪便短链脂肪酸（SCFAs）及toll样受体4（TLR4）/髓样分化因子88（MyD88）/核因子κB（NF-κB）信号通路的影响，探讨针刺降压的作用机制。

方法：

将24只SPF级雄性SHR随机分为模型组、西药组、针刺组和假针组，每组6只；另设6只Wistar-Kyoto（WKY）大鼠为空白组。西药组予氢氯噻嗪溶液灌胃；针刺组针刺双侧“人迎”“足三里”，每次20 min；假针组针刺双侧“人迎”“足三里”附近的非经非穴点，每次20 min。均每日1次，持续4周。干预前及干预第1、2、3、4周后测量各组大鼠尾动脉收缩压（SBP）；HE染色观察大鼠结肠组织形态；气相色谱-质谱联用仪检测大鼠粪便SCFAs含量；ELISA法检测大鼠血清白细胞介素（IL）-6、IL-1β和肿瘤坏死因子-α（TNF-α）含量；Western Blot法检测大鼠肠系膜动脉中TLR4、MyD88和NF-κB p65蛋白表达。

结果：

与空白组比较，模型组大鼠SBP升高（

0.05），结肠组织有明显病理变化，粪便SCFAs含量降低（

0.05），血清

IL-1β、IL-6和TNF-α含量升高（

0.05），肠系膜动脉中TLR4、MyD88、NF-κB p65蛋白表达升高（

0.05）。与模型组比较，针刺组和西药组大鼠干预2、3、4周后SBP降低（

0.05），血清IL-1β、IL-6和TNF-α含量降低（

0.05）；针刺组大鼠结肠组织黏膜上皮完整，肠腺数量丰富，粪便SCFAs含量升高（

0.05），肠系膜动脉中TLR4、MyD88、NF-κB p65蛋白表达降低（

0.05）。与假针组比较，针刺组干预2、3、4周后SBP降低（

0.05），粪便SCFAs含量升高（

0.05），血清IL-1β、IL-6和TNF-α含量降低（

0.05），肠系膜动脉中TLR4、MyD88、NF-κB p65蛋白表达降低（

0.05）。

结论：

针刺双侧“人迎”“足三里”可有效降低SHR血压，其机制可能与调节粪便SCFAs含量、抑制TLR4/MyD88/NF-κB信号通路有关。

Abstract

Objective

To observe the effects of acupuncture on blood pressure

fecal short-chain fatty acids (SCFAs) and toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear factor-κB (NF-κB) signaling pathway in spontaneously hypertensive rats (SHR)

and to explore the mechanism of acupuncture for anti-hypertension.

Methods

Twenty-four male SHR of SPF grade were randomly divided into a model group

a western medication group

an acupuncture group and a sham acupuncture group

with 6 rats in each group

and 6 male Wistar-Kyoto rats were selected as the blank group additionally. Hydrochlorothiazide solution was given by gavage in the western medication group; acupuncture was applied at bilateral "Renying" (ST 9) and "Zusanli" (ST 36) in the acupuncture group

20 min a time; acupuncture was applied at the non-meridian and non-acupoint points close to bilateral "Renying" (ST 9) and "Zusanli" (ST 36) in the sham acupuncture group

20 min a time. The intervention was adopted once a day for 4 weeks continuously in each group. The systolic blood pressure (SBP) of the caudal artery was measured before intervention and after 1

3 and 4 weeks of intervention. After intervention

the morphology of colonic tissue was observed by HE staining; the fecal level of SCFAs was detected by gas chromatography; the serum levels of interleukin (IL)-6

IL-1βand tumor necrosis factor-α (TNF-α) were detected by ELISA; the protein expression of TLR4

MyD88 and NF-κB p65 in the mesenteric artery was detected by Western blot.

Results

Compared with the blank group

in the model group

the SBP was increased (

0.05)

significant pathological changes could be found in the colonic tissue

the fecal SCFAs level was decreased (

0.05)

the serum levels of IL-6

IL-1β and TNF-α were increased (

0.05)

the protein expression of TLR4

MyD88 and NF-κB p65 in the mesenteric artery was increased (

0.05). Compared with the model group

the SBP after 2

3 and 4 weeks of intervention was decreased (

0.05)

the serum levels of IL-6

IL-1β and TNF-α were decreased (

0.05) in the acupuncture group and the western medication group; the mucosal epithelium of colonic tissue was intact

the number of intestinal glands was abundant

the fecal SCFAs level was increased (

0.05)

and the protein expression of TLR4

MyD88 and NF-κB p65 in the mesenteric artery was decreased (

0.05) in the acupuncture group. Compared with the sham acupuncture group

the SBP after 2

3 and 4 weeks of intervention was decreased (

0.05)

the fecal SCFAs level was increased (

0.05)

the serum levels of IL-6

IL-1β and TNF-α were decreased (

0.05)

the protein expression of TLR4

MyD88 and NF-κB p65 in the mesenteric artery was decreased (

0.05) in the acupuncture group.

Conclusion

Acupuncture at bilateral "Renying" (ST 9) and "Zusanli" (ST 36) can effectively play an anti-hypertensive role in SHR. Its mechanism may be related to regulating fecal SCFAs level and inhibiting the TLR4/MyD88/NF-κB signaling pathway.

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