电针“肠病方”对急性溃疡性结肠炎大鼠NLRP3炎性小体和肠黏膜屏障的影响

蒋子文; 武荣林; 徐慧超; 王海军; 董爱爱; 冀来喜

doi:10.13703/j.0255-2930.20230716-k0001

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电针“肠病方”对急性溃疡性结肠炎大鼠NLRP3炎性小体和肠黏膜屏障的影响

机制探讨 | 更新时间：2024-06-03

- 电针“肠病方”对急性溃疡性结肠炎大鼠NLRP3炎性小体和肠黏膜屏障的影响
- Effects of electroacupuncture with "intestinal disease prescription" on NLRP3 inflammasome and intestinal mucosal barrier in rats with acute ulcerative colitis
- 中国针灸 2024年44卷第4期页码：441-448
- 作者机构：
  
  1.山西中医药大学第二临床学院，太原030619
  2.成都中医药大学针灸推拿学院
- 作者简介：
  
  蒋子文，山西中医药大学硕士研究生。E-mail：1144589546@qq.com
  冀来喜，教授。E-mail：tyjilaixi@126.com
- 基金信息：
  
  国家自然科学基金资助项目(82074549)
- DOI：10.13703/j.0255-2930.20230716-k0001
  中图分类号：
- 收稿：2023-07-16，
  
  网络首发：2024-02-06，
  
  纸质出版：2024-04-12
- 稿件说明：
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蒋子文,武荣林,徐慧超,等.电针“肠病方”对急性溃疡性结肠炎大鼠NLRP3炎性小体和肠黏膜屏障的影响[J].中国针灸,2024,44(4):441-448.

JIANG Ziwen,WU Ronglin,XU Huichao,et al.Effects of electroacupuncture with "intestinal disease prescription" on NLRP3 inflammasome and intestinal mucosal barrier in rats with acute ulcerative colitis[J].Chinese Acupuncture & Moxibustion,2024,44(04):441-448.
蒋子文,武荣林,徐慧超,等.电针“肠病方”对急性溃疡性结肠炎大鼠NLRP3炎性小体和肠黏膜屏障的影响[J].中国针灸,2024,44(4):441-448. DOI： 10.13703/j.0255-2930.20230716-k0001.

JIANG Ziwen,WU Ronglin,XU Huichao,et al.Effects of electroacupuncture with "intestinal disease prescription" on NLRP3 inflammasome and intestinal mucosal barrier in rats with acute ulcerative colitis[J].Chinese Acupuncture & Moxibustion,2024,44(04):441-448. DOI： 10.13703/j.0255-2930.20230716-k0001.

摘要

目的

观察电针“肠病方”对葡聚糖硫酸钠（DSS）诱导的急性溃疡性结肠炎（UC）大鼠肠黏膜屏障和核苷酸结合寡聚化结构域样受体蛋白3（NLRP3）炎性小体的影响，探讨电针“肠病方”治疗UC的作用机制。

方法

将32只SPF级健康雄性SD大鼠随机分成空白组、模型组、西药组和电针组，每组8只。除空白组外，大鼠饮用5%DSS溶液7 d制备UC模型。造模后，西药组予美沙拉嗪混悬液（200 mg/kg）灌胃；电针组予针刺双侧“天枢”“上巨虚”和“中脘”穴，同侧“天枢”与“上巨虚”连接电子针疗仪电极，予疏密波，频率10 Hz/50 Hz，每次干预20 min。两组均每日1次，干预3 d。每天观察各组大鼠一般情况。干预后，计算大鼠疾病活动指数（DAI）评分；HE染色法观察大鼠结肠组织形态；ELISA法检测血清促炎性细胞因子［白细胞介素（IL）-18、IL-1β］含量；Western blot法检测大鼠结肠组织NLRP3、凋亡相关斑点样蛋白（ASC）、半胱氨酸天冬氨酸蛋白酶-1（Caspase-1）蛋白表达；免疫荧光法检测结肠组织闭锁连接蛋白-1（ZO-1）、闭锁蛋白（Occludin）阳性表达。

结果

与空白组比较，模型组大鼠一般状况较差，体质量增长缓慢，结肠长度缩短（

0.01），DAI评分与脾脏指数升高（

0.01），血清IL-18、IL-1β含量与结肠组织NLRP3、ASC、Caspase-1蛋白表达升高（

0.01），结肠组织ZO-1、Occludin阳性表达减少（

0.01）。与模型组比较，电针组与西药组大鼠的一般状况改善，体质量增长加快，结肠长度增加（

0.05），DAI评分与脾脏指数降低（

0.05），血清IL-18、IL-1β含量与结肠组织NLRP3、ASC、Caspase-1蛋白表达降低（

0.05），结肠组织ZO-1、Occludin阳性表达增加（

0.05）。西药组和电针组以上指标比较，差异无统计学意义（

0.05）。与空白组比较，模型组结肠黏膜形态破坏，腺体排列紊乱，隐窝萎缩，有明显的炎性细胞浸润；与模型组比较，西药组和电针组大鼠结肠黏膜形态相对完好，腺体与隐窝结构得到了恢复与改善，炎性细胞浸润减少。

结论

电针“肠病方”对DSS诱导的急性UC具有显著的改善作用，可能是通过调节NLRP3炎性小体和肠黏膜屏障相关蛋白的表达，从而减轻溃疡性结肠炎的症状。

Abstract

Objective

To observe the effects of electroacupuncture (EA) with "intestinal disease prescription" on the intestinal mucosal barrier and NLRP3 inflammasome in rats with dextran sulfate sodium (DSS)-induced acute ulcerative colitis (UC)

and explore the underlying mechanism of EA with "intestinal disease prescription" for the treatment of UC.

Methods

Thirty-two healthy male SPF-grade SD rats were randomly divided into a blank group

a model group

a medication group

and an EA group

with 8 rats in each group. Except for the blank group

the UC model was established by administering 5% DSS solution for 7 days. After modeling

the rats in the medication group were treated with mesalazine suspension (200 mg/kg) by gavage

while the rats in the EA group were treated with acupuncture at bilateral "Tianshu" (ST 25)

"Shangjuxu" (ST 37) and "Zhongwan" (CV 12)

with the ipsilateral "Tianshu" (ST 25) and "Shangjuxu" (ST 37) connected to the electrodes of the EA instrument

using disperse-dense wave

with a frequency of 10 Hz/50 Hz

and each intervention lasted for 20 minutes. Both interventions were performed once daily for 3 days. The general conditions of rats were observed daily. After intervention

the disease activity index (DAI) score was calculated; colon tissue morphology was observed using HE staining; serum levels of pro-inflammatory cytokines (interleukin [IL]-18

IL-1β) were measured by ELISA; protein expression of NLRP3

apoptosis-associated speck-like protein containing a CARD (ASC)

and Caspase-1 in colon tissues was detected by Western blot; positive expression of zonula occludens-1 (ZO-1) and Occludin in colon tissues was examined by immunofluorescence.

Results

Compared with the blank group

the rats in the model group exhibited poor general conditions

slow body weight gain

shortened colon length (

0.01)

increased DAI score and spleen index (

0.01)

elevated serum IL-18 and IL-1β levels

and increased protein expression of NLRP3

ASC

and Caspase-1 in colon tissues (

0.01)

along with decreased positive expression of ZO-1 and Occludin in colon tissues (

0.01). Compared with the model group

the rats in the medication group and the EA group exhibited improved gener

al conditions

accelerated body weight gain

increased colon length (

0.05)

reduced DAI scores and spleen indexes (

0.05)

decreased serum IL-18 and IL-1β levels

and lower protein expression of NLRP3

ASC and Caspase-1 in colon tissues (

0.05)

as well as increased positive expression of ZO-1 and Occludin in colon tissues (

0.05). There were no significant differences in the above indexes between the medication group and the EA group (

0.05). Compared with the blank group

the rats in the model group exhibited disrupted colon mucosal morphology

disordered gland arrangement

and atrophy of crypts

along with significant inflammatory cell infiltration. Compared with the model group

the rats in both the medication group and the EA group showed relatively intact colon mucosal morphology

with restored and improved gland and crypt structures

and reduced inflammatory cell infiltration.

Conclusion

EA with "intestinal disease prescription" has a significant therapeutic effect on DSS-induced UC

possibly by regulating the expression of NLRP3 inflammasome and proteins related to the intestinal mucosal barrier

thereby alleviating symptoms of ulcerative colitis.

关键词

Keywords

references

Kaplan GG . The global burden of IBD: from 2015 to 2025 [J ] . Nat Rev Gastroenterol Hepatol , 2015 , 12 ( 12 ): 720 - 727 .

Ungaro R , Mehandru S , Allen PB , et al . Ulcerative colitis [J ] . Lancet , 2017 , 389 ( 10080 ): 1756 - 1770 .

金淑萍 , 王红梅 , 于洋 . 美沙拉嗪不良反应文献概述 [J ] . 中国药物滥用防治杂志 , 2017 , 23 ( 4 ): 239 - 240 .

张浩冉 , 崔海栋 , 梁国英 . 生物制剂在溃疡性结肠炎治疗中应用的研究进展 [J ] . 国际消化病杂志 , 2023 , 43 ( 4 ): 213 - 216 .

李嫣红 , 曹晖 . 针灸联合中医药治疗溃疡性结肠炎临床疗效的Meta分析 [J ] . 中医临床研究 , 2022 , 14 ( 34 ): 98 - 101 .

Foerster EG , Mukherjee T , Cabral-Fernandes L , et al . How autophagy controls the intestinal epithelial barrier [J ] . Autophagy , 2022 , 18 ( 1 ): 86 - 103 .

Wang J , Zhang CL , Guo CM , et al . Chitosan ameliorates DSS-induced ulcerative colitis mice by enhancing intestinal barrier function and improving microflora [J ] . Int J Mol Sci , 2019 , 20 ( 22 ): 5751 .

陆璐 , 刘宇婧 , 李瑗 , 等 . 基于NLRP3相关炎症反应和肠道黏膜屏障功能探究汉黄芩素治疗溃疡性结肠炎小鼠的作用机制 [J ] . 中华中医药杂志 , 2022 , 37 ( 10 ): 5992 - 5999 .

Zhang J , Liu XQ , Wan CY , et al . NLRP3 inflammasome mediates M1 macrophage polarization and IL-1β production in inflammatory root resorption [J ] . J Clin Periodontol , 2020 , 47 ( 4 ): 451 - 460 .

王倩 , 包永欣 . 2种质量浓度DSS致急性溃疡性结肠炎大鼠模型的构建与比较 [J ] . 广州中医药大学学报 , 2019 , 36 ( 4 ): 574 - 577 .

徐叔云 . 药理实验方法学 [M ] . 3版 . 北京 : 人民卫生出版社 , 2002 .

郭义 . 实验针灸学 [M ] . 北京 : 中国中医药出版社 , 2008 : 3 .

Bang B , Lichtenberger LM . Methods of inducing inflammatory bowel disease in mice [J ] . Curr Protoc Pharmacol , 2016 , 72: 5.58. 1 - 5 .58.42.

于树涛 , 李玲 , 周荣柱 , 等 . 乳酸菌发酵饲料对蛋鸡脏器指数及血液抗氧化和免疫指标的影响 [J ] . 中国饲料 , 2023 ( 11 ): 124 - 128 .

刘梦茹 , 李慧 , 魏兰福 , 等 . 黄芩汤对溃疡性结肠炎模型小鼠NLRP3/Caspase-1通路的影响 [J ] . 中国中药杂志 , 2023 , 48 ( 1 ): 226 - 233 .

刘喜梅 . 益生菌联合美沙拉嗪治疗轻中度溃疡性结肠炎的效果及安全性探讨 [J ] . 中国现代药物应用 , 2022 , 16 ( 18 ): 133 - 135 .

程艳婷 , 路晓红 , 王艳珍 , 等 . 中脘、天枢、上巨虚为肠病基本方的古代文献研究 [J ] . 江苏中医药 , 2011 , 43 ( 7 ): 79 - 80 .

闫丽萍 , 冀来喜 , 王海军 , 等 . 电针治疗肠黏膜损伤大鼠基本腧穴配伍“肠病方”的筛选 [J ] . 针刺研究 , 2012 , 37 ( 6 ): 458 - 463 .

Wan F , Wang MY , Zhong RQ , et al . Supplementation with Chinese medicinal plant extracts from lonicera hypoglauca and scutellaria baicalensis mitigates colonic inflammation by regulating oxidative stress and gut microbiota in a colitis mouse model [J ] . Front Cell Infect Microbiol , 2021 , 11 : 798052 .

Zhu L , Gu PQ , Shen H . Protective effects of berberine hydrochloride on DSS-induced ulcerative colitis in rats [J ] . Int Immunopharmacol , 2019 , 68 : 242 - 251 .

Linghu KG , Ma QS , Xiong SH , et al . The "whole ingredients extract" of astragali radix improves the symptoms of dextran sulfate sodium-induced ulcerative colitis in mice through systemic immunomodulation [J ] . Chin Med , 2022 , 17 ( 1 ): 109 .

刘坤 , 徐菁 , 马竹芳 . 溃疡性结肠炎血清IGF-1、IL-6、IL-18表达水平的变化及意义 [J ] . 临床和实验医学杂志 , 2023 , 22 ( 2 ): 140 - 143 .

徐素芳 , 张英 , 赵小燕 . 溃疡性结肠炎患者肠道菌群与血清IFN-γ、IL-1β、sIL-2R水平的关系 [J ] . 检验医学与临床 , 2023 , 20 ( 5 ): 664 - 667,672 .

Zhen Y , Zhang H . NLRP3 inflammasome and inflammatory bowel disease [J ] . Front Immunol , 2019 , 10 : 276 .

Kelley N , Jeltema D , Duan YH , et al . The NLRP3 inflammasome: an overview of mechanisms of activation and regulation [J ] . Int J Mol Sci , 2019 , 20 ( 13 ): 3328 .

Huang Y , Xu W , Zhou RB . NLRP3 inflammasome activation and cell death [J ] . Cell Mol Immunol , 2021 , 18 ( 9 ): 2114 - 2127 .

Zhong ZY , Umemura A , Sanchez-Lopez E , et al . NF-κB restricts inflammasome activation via elimination of damaged mitochondria [J ] . Cell , 2016 , 164 ( 5 ): 896 - 910 .

Cosin-Roger J , Simmen S , Melhem H , et al . Hypoxia ameliorates intestinal inflammation through NLRP3/mTOR downregulation and autophagy activation [J ] . Nat Commun , 2017 , 8 ( 1 ): 98 .

Shao XY , Sun SS , Zhou YQ , et al . Bacteroides fragilis restricts colitis-associated cancer via negative regulation of the NLRP3 axis [J ] . Cancer Lett , 2021 , 523 : 170 - 181 .

Kayama H , Okumura R , Takeda K . Interaction between the microbiota, epithelia, and immune cells in the intestine [J ] . Annu Rev Immunol , 2020 , 38 : 23 - 48 .

Martens EC , Neumann M , Desai MS . Interactions of commensal and pathogenic microorganisms with the intestinal mucosal barrier [J ] . Nat Rev Microbiol , 2018 , 16 ( 8 ): 457 - 470 .

Qu SW , Fan LN , Qi YD , et al . Akkermansia muciniphila alleviates dextran sulfate sodium (DSS)-induced acute colitis by NLRP3 activation [J ] . Microbiol Spectr , 2021 , 9 ( 2 ): e0073021 .

Barbara G , Barbaro MR , Fuschi D , et al . Inflammatory and microbiota-related regulation of the intestinal epithelial barrier [J ] . Front Nutr , 2021 , 8 : 718356 .

Cui L , Guan XN , Ding WB , et al . Scutellaria baicalensis Georgi polysaccharide ameliorates DSS-induced ulcerative colitis by improving intestinal barrier function and modulating gut microbiota [J ] . Int J Biol Macromol , 2021 , 166 : 1035 - 1045 .

Wu H , Chen QY , Wang WZ , et al . Compound sophorae decoction enhances intestinal barrier function of dextran sodium sulfate induced colitis via regulating Notch signaling pathway in mice [J ] . Biomed Pharmacother , 2021 , 133 : 110937 .

徐雪飞 , 王恩力 , 杨田野 , 等 . 基于肠黏膜屏障及TLR4/NF-κB/NLRP3信号通路探讨燮和饮改善PCOS-IR小鼠炎症反应的影响 [J ] . 中国实验方剂学杂志 , 2021 , 27 ( 17 ): 90 - 98 .

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