电针抑制Notch1/Hes1通路介导的小胶质细胞活化对帕金森病小鼠的影响

蒋金序; 刘洋; 樊慧杰; 张天生; 王利然; 徐磊; 杨丽霞; 宋云飞; 马存根; 郝重耀; 柴智

doi:10.13703/j.0255-2930.20240528-0002

您当前的位置：

首页 >

文章列表页 >

电针抑制Notch1/Hes1通路介导的小胶质细胞活化对帕金森病小鼠的影响

机制探讨 | 更新时间：2025-09-12

- 电针抑制Notch1/Hes1通路介导的小胶质细胞活化对帕金森病小鼠的影响
- Inhibitory effect of electroacupuncture on microglial activation via Notch1/Hes1 pathway in Parkinson’s disease mice
- 中国针灸 2025年45卷第9期页码：1290-1298
- 作者机构：
  
  山西中医药大学第二临床学院，太原　030012
  山西中医药大学多发性硬化益气活血重点研究室/神经生物学研究中心，晋中　030619
- 作者简介：
  
  蒋金序，山西中医药大学硕士研究生。E-mail：jiangjinxu@stu.sxtcm.edu.cn
  ✉柴智，教授。E-mail：chaizhi008@126.com
- 基金信息：
  
  国家中医药管理局青年岐黄学者培养项目(国中医药人教函〔2022〕256号);山西省重点科技创新人才团队专项(202304051001019);山西省重点国别科技合作项目(202204041101002);山西省青年拔尖人才支持计划项目(晋组办字〔2019〕35号文);山西省省筹资金资助回国留学人员科研项目(2021-142);山西省卫健委中医药重点研究室建设专项(zyyyjs2024022);山西省卫健委中医药创新团队建设专项(zyytd2024022);山西中医药大学科技创新团队建设项目(2022TD1013)
- DOI：10.13703/j.0255-2930.20240528-0002
  中图分类号：
- 收稿：2024-05-28，
  
  网络首发：2025-07-17，
  
  纸质出版：2025-09-12
- 稿件说明：
移动端阅览
蒋金序, 刘洋, 樊慧杰, 等. 电针抑制Notch1/Hes1通路介导的小胶质细胞活化对帕金森病小鼠的影响[J]. 中国针灸, 2025,45(9):1290-1298.

JIANG Jinxu, LIU Yang, FAN Huijie, et al. Inhibitory effect of electroacupuncture on microglial activation via Notch1/Hes1 pathway in Parkinson’s disease mice[J]. Chinese Acupuncture & Moxibustion, 2025, 45(9): 1290-1298.
蒋金序, 刘洋, 樊慧杰, 等. 电针抑制Notch1/Hes1通路介导的小胶质细胞活化对帕金森病小鼠的影响[J]. 中国针灸, 2025,45(9):1290-1298. DOI： 10.13703/j.0255-2930.20240528-0002.

JIANG Jinxu, LIU Yang, FAN Huijie, et al. Inhibitory effect of electroacupuncture on microglial activation via Notch1/Hes1 pathway in Parkinson’s disease mice[J]. Chinese Acupuncture & Moxibustion, 2025, 45(9): 1290-1298. DOI： 10.13703/j.0255-2930.20240528-0002.

摘要

目的

观察电针（EA）对帕金森病（PD）小鼠运动功能的改善作用，以及基于Notch受体1（Notch1）/ Hes家族bHLH转录因子1（Hes1）通路对小胶质细胞的调控效应。

方法

选取36只雄性C57BL/6小鼠随机分为对照组、模型组和电针组，每组12只。模型组和电针组小鼠采用1-甲基-4-苯基-1

6-四氢吡啶（MPTP）连续腹腔注射7 d建立PD模型。自模型制备首日起电针组于“百会”和双侧“肾俞”行电针干预，采用连续波，频率2 Hz，电流2 mA，每次15 min，每天1次，连续14 d。采用步态、爬杆和悬挂实验对各组小鼠行为学表现进行评估；采用免疫荧光法检测各组小鼠中脑黑质区酪氨酸羟化酶（TH）阳性细胞数以及Notch1/离子钙结合接头分子1（Iba-1）共表达阳性细胞数；采用Western blot法检测各组小鼠中脑黑质区TH、α-突触核蛋白（α-syn）、Notch1、Hes1、Iba-1、诱导型一氧化氮合酶（iNOS）、精氨酸酶-1（ARG1）、肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β、IL-6、IL-10蛋白表达；采用实时荧光定量PCR法检测各组小鼠中脑黑质区Notch1和Hes1 mRNA表达。

结果

与对照组比较，模型组小鼠四肢步频加快（

0.001），四肢步长缩短（

0.001），爬杆实验时间延长（

0.01），抓握实验评分降低（

0.01）；中脑黑质区TH阳性细胞数减少（

0.001），Notch1/Iba-1共表达阳性细胞数增多（

0.001），α-syn、Notch1、Hes1、Iba-1、iNOS、TNF-α、IL-6和IL-1β蛋白表达升高（

0.01，

0.05，

0.001），TH、ARG1和IL-10蛋白表达降低（

0.01，

0.001），Notch1、Hes1 mRNA表达升高（

0.01）。与模型组比较，电针组小鼠四肢步频减慢（

0.001），四肢步长增加（

0.05，

0.01，

0.001），爬杆实验时间缩短（

0.05），抓握实验评分升高（

0.05）；中脑黑质区TH阳性细胞数增多（

0.01），Notch1/Iba-1共表达阳性细胞数减少（

0.001），α-syn、Notch1、Hes1、Iba-1、iNOS、TNF-α、IL-6和IL-1β蛋白表达降低（

0.05，

0.01），TH、ARG1和IL-10蛋白表达升高（

0.05，

0.001，

0.01），Notch1、Hes1 mRNA表达降低（

0.05）。

结论

电针干预能够改善PD模型小鼠的行为学表现，保护多巴胺能神经元，其机制可能与通过Notch1/Hes1信号通路抑制神经炎性反应，进而减少小胶质细胞活化有关。

Abstract

Objective

To observe the effects of electroacupuncture (EA) on improving motor function and regulating microglial activation based on Notch receptor 1 (Notch1)/Hes family bHLH transcription factor 1 (Hes1) pathway in mice with Parkinson’s disease (PD).

Methods

Thirty-six male C57BL/6 mice were randomly divided into a control group

a model group and an EA group

12 mice in each group. PD model was established by intraperitoneal injection of 1-methyl-4-phenyl-1

6-tetrahydropyridine (MPTP) for 7 consecutive days in the model group and the EA group. From the 1st day of modeling

EA was applied at "Baihui" (GV20) and bilateral "Shenshu" (BL23) in the EA group

with continuous wave

in frequency of 2 Hz and current of 2 mA

15 min a time

once a day for 14 days continuously. The behavioral performance was evaluated by gait test

pole climbing test and hanging test

the number of positive cells of tyrosine hydroxylase (TH) and the co-expression positive cells of Notch1/ionized calcium binding adaptor molecule 1 (Iba-1) in the substantia nigra of midbrain was assessed by immunofluorescence

the protein expression of TH

α-synuclein (α-syn)

Notch1

Hes1

Iba-1

inducible nitric oxide synthase (iNOS)

Arginase-1 (ARG1)

tumor necrosis factor-α (TNF-α)

interleukin (IL)-1β

IL-6 and IL-10 was detected by Western blot

the mRNA expression of Notch1 and Hes1 was detected by real-time PCR.

Results

Compared with the control group

in the model group

the stride frequency was accelerated (

0.001) and the stride length was shortened (

0.001) for the four limbs

the pole climbing test time was prolonged (

0.01) and the grip level was reduced (

0.01); in the substantia nigra of midbrain

the number of positive cells of TH was decreased (

0.001)

the number of co-expression positive cells of Notch1/Iba-1 was increased (

0.001)

the protein expression of α-syn

Notch1

Hes1

Iba-1

iNOS

TNF-α

IL-1βand IL-6 was increased (

0.01

0.05

0.001)

the protein expression of TH

ARG1 and IL-10 was decreased (

0.01

0.001)

the mRNA expression of Notch1 and Hes1 was increased (

0.01). Compared with the model group

in the EA group

the stride frequency was decelerated (

0.001) and the stride length was increased (

0.05

0.01

0.001) for the four limbs

the pole climbing test time was shortened (

0.05) and the grip level was increased (

0.05); in the substantia nigra of midbrain

the number of positive cells of TH was increased (

0.01)

the number of co-expression positive cells of Notch1/Iba-1 was decreased (

0.001)

the protein expression of α-syn

Notch1

Hes1

Iba-1

iNOS

TNF-α

IL-6 and IL-1β was decreased (

0.05

0.01)

the protein expression of TH

ARG1 and IL-10 was increased (

0.05

0.001

0.01)

the mRNA expression of Notch1 and Hes1 was decreased (

0.05).

Conclusion

EA can improve the behavioral performance and protect the dopaminergic neurons in PD mice

its mechanism may relate to the inhibition of Notch1/Hes1-mediated neuroinflammation

thus inhibiting the microglial activation.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

电针对腰椎间盘退变模型兔腰段脊髓小胶质细胞活化的影响

电针对KOA慢性疼痛伴痛情绪大鼠前扣带回小胶质细胞活性及其极化表型的影响

“嗅三针”调控小胶质细胞改善血管性痴呆大鼠髓鞘再生的机制研究

电针调控mTOR/SREBP1信号通路促进肥胖小鼠白色脂肪棕色化的机制研究

“标本配穴”电针预处理减轻心肌缺血再灌注损伤大鼠心肌细胞铜死亡的机制研究