艾灸“肺俞”“心俞”对慢性心力衰竭大鼠心肌circPAN3、FOXO3、BNIP3水平及心肌纤维化的影响

李澜; 高兵; 胡婧; 刘攀; 李丽雅; 李瑞华; 王茎

doi:10.13703/j.0255-2930.20241028-k0002

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艾灸“肺俞”“心俞”对慢性心力衰竭大鼠心肌circPAN3、FOXO3、BNIP3水平及心肌纤维化的影响

机制探讨 | 更新时间：2025-11-20

- 艾灸“肺俞”“心俞”对慢性心力衰竭大鼠心肌circPAN3、FOXO3、BNIP3水平及心肌纤维化的影响
- Effects of moxibustion at "Feishu" (BL13) and "Xinshu" (BL15) on myocardial circPAN3, FOXO3, BNIP3 levels and myocardial fibrosis in rats with chronic heart failure
- 中国针灸 2025年45卷第11期页码：1600-1608
- 作者机构：
  
  安徽中医药大学针灸推拿学院，合肥　230012
  安徽中医药大学新安医学与中医药现代化研究所，合肥　230000
  安徽中医药大学中医学院，合肥　230012
  安徽中医药大学新安医学教育部重点实验室，合肥　230000
- 作者简介：
  
  李澜，安徽中医药大学硕士研究生。E-mail：19965069475@163.com
  ✉王茎，教授。E-mail：wangjing2161@126.com
- 基金信息：
  
  安徽省教育厅重点项目(KJ2021A0570;2023AH050796);大健康研究院新安医学与中医药现代化研究所专项项目(2023CXMMTCM022)
- DOI：10.13703/j.0255-2930.20241028-k0002
  中图分类号：
- 收稿：2024-10-28，
  
  网络首发：2025-02-07，
  
  纸质出版：2025-11-12
- 稿件说明：
移动端阅览
李澜, 高兵, 胡婧, 等. 艾灸“肺俞”“心俞”对慢性心力衰竭大鼠心肌circPAN3、FOXO3、BNIP3水平及心肌纤维化的影响[J]. 中国针灸, 2025,45(11):1600-1608.

LI Lan, GAO Bing, HU Jing, et al. Effects of moxibustion at "Feishu" (BL13) and "Xinshu" (BL15) on myocardial circPAN3, FOXO3, BNIP3 levels and myocardial fibrosis in rats with chronic heart failure[J]. Chinese Acupuncture & Moxibustion, 2025, 45(11): 1600-1608.
李澜, 高兵, 胡婧, 等. 艾灸“肺俞”“心俞”对慢性心力衰竭大鼠心肌circPAN3、FOXO3、BNIP3水平及心肌纤维化的影响[J]. 中国针灸, 2025,45(11):1600-1608. DOI： 10.13703/j.0255-2930.20241028-k0002.

LI Lan, GAO Bing, HU Jing, et al. Effects of moxibustion at "Feishu" (BL13) and "Xinshu" (BL15) on myocardial circPAN3, FOXO3, BNIP3 levels and myocardial fibrosis in rats with chronic heart failure[J]. Chinese Acupuncture & Moxibustion, 2025, 45(11): 1600-1608. DOI： 10.13703/j.0255-2930.20241028-k0002.

摘要

目的：

观察艾灸“肺俞”“心俞”对慢性心力衰竭（CHF）大鼠心肌Pan3基因第2-5外显子的环状RNA（circPAN3）、叉头框O3蛋白（FOXO3）及B淋巴细胞瘤-2/腺病毒E1B19kDa相互作用蛋白3（BNIP3）的影响，探讨艾灸减轻心肌纤维化的潜在作用机制。

方法：

60只SPF级雄性SD大鼠随机取10只作为正常组，剩余大鼠采用冠状动脉左前降支（LAD）结扎术制备CHF模型，将模型制备成功的40只大鼠随机分为模型组、艾灸组、雷帕霉素（RAPA）组、艾灸+RAPA组，每组10只。艾灸组予温和灸双侧“肺俞”“心俞”，每次30 min；RAPA组腹腔注射自噬激动剂RAPA（1 mg/kg）；艾灸+RAPA组先腹腔注射RAPA，后予温和灸双侧“肺俞”“心俞”。均每日干预1次，连续4周。干预结束后采用心脏彩超检测大鼠射血分数（EF）、左心室短轴缩短率（FS）；ELISA法检测大鼠血清胎盘生长因子（PLGF）含量；HE染色、Masson染色观察大鼠心肌组织形态及胶原容积；实时荧光定量PCR法检测大鼠心肌组织circPAN3、FOXO3、BNIP3 mRNA表达；Western blot法检测大鼠心肌组织FOXO3、BNIP3蛋白表达。

结果：

与正常组比较，模型组大鼠心肌细胞排列紊乱，纤维化严重，胶原容积增加（

0.01）；EF、FS值及心肌组织circPAN3 mRNA表达降低（

0.01），血清PLGF含量及心肌组织FOXO3、BNIP3 mRNA和蛋白表达升高（

0.01）。与模型组比较，艾灸组大鼠心肌纤维化程度减轻，胶原容积降低（

0.01）；EF、FS值及心肌组织circPAN3 mRNA表达升高（

0.01），血清PLGF含量及心肌组织FOXO3、BNIP3 mRNA和蛋白表达降低（

0.01）。与模型组比较，RAPA组大鼠相关指标进一步恶化（

0.01）。与RAPA组比较，艾灸+RAPA组大鼠心肌纤维化程度减轻，胶原容积降低（

0.01）；EF、FS值及心肌组织circPAN3 mRNA表达升高（

0.01），血清PLGF含量及心肌组织FOXO3、BNIP3 mRNA和蛋白表达降低（

0.01）。

结论：

艾灸可减轻CHF大鼠心肌纤维化，其机制可能与上调心肌组织circPAN3表达，降低FOXO3、BNIP3表达，抑制心肌细胞过度自噬有关。

Abstract

Objective

To observe the effects of moxibustion at "Feishu" (BL13) and "Xinshu" (BL15) on the circular RNA of exon 2-5 of the Pan3 gene (circPAN3)

forkhead box O3 (FOXO3)

and Bcl-2/adenovirus E1B19kDa-interacting protein 3 (BNIP3) in rats with chronic heart failure (CHF)

and explore the potential mechanisms of moxibustion in alleviating myocardial fibrosis.

Methods

Ten rats of 60 male SPF-grade SD rats were randomly assigned into a normal group. The remaining rats underwent left anterior descending coronary artery (LAD) ligation to establish the CHF model. Forty successfully modeled rats were randomly divided into a model group

a moxibustion group

a rapamycin (RAPA) group

and a moxibustion+RAPA group

with 10 rats in each group. The moxibustion group received mild moxibustion at bilateral "Feishu" (BL13) and "Xinshu" (BL15)

30 min per session. The RAPA group received intraperitoneal injection of the autophagy activator RAPA (1 mg/kg). The moxibustion+RAPA group first received RAPA injection

followed by mild moxibustion at bilateral "Feishu" (BL13) and "Xinshu" (BL15). All interventions were administered once daily for 4 consecutive weeks. After the intervention

cardiac ultrasound was used to measure ejection fraction (EF) and left ventricular fractional shortening (FS). Serum placental growth factor (PLGF) level was determined by ELISA. Myocardial tissue morphology and collagen volume were assessed using hematoxylin-eosin (HE) staining and Masson's trichrome staining. The expression levels of circPAN3

FOXO3

and BNIP3 mRNA in myocardial tissue were detected by real-time PCR

while FOXO3 and BNIP3 protein expression levels were analyzed by Western blot.

Results

Compared with the normal group

the model group exhibited myocardial cell disorder

severe fibrosis

and increased collagen volume (

0.01)

along with significantly decreased EF

and c

ircPAN3 mRNA expression in myocardial tissue (

0.01)

and the serum PLGF level

as well as FOXO3 and BNIP3 mRNA and protein expression in myocardial tissue were increased (

0.01). Compared with the model group

the moxibustion group showed reduced myocardial fibrosis

decreased collagen volume (

0.01)

increased EF

and circPAN3 mRNA expression in myocardial tissue (

0.01)

and decreased serum PLGF level as well as FOXO3 and BNIP3 mRNA and protein expression in myocardial tissue (

0.01). Compared with the model group

the RAPA group showed further deterioration in these parameters (

0.01). Compared with the RAPA group

the moxibustion+RAPA group exhibited alleviation of myocardial fibrosis

reduced collagen volume (

0.01)

increased EF

and circPAN3 mRNA expression in myocardial tissue (

0.01)

and decreased serum PLGF level as well as FOXO3 and BNIP3 mRNA and protein expression in myocardial tissue (

0.01).

Conclusion

Moxibustion could alleviate myocardial fibrosis in CHF rats

possibly through upregulation of myocardial circPAN3 expression

downregulation of FOXO3 and BNIP3 expression

and inhibition of excessive myocardial autophagy.

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