电针“内关”对心肌缺血再灌注损伤大鼠缺血期和再灌注期线粒体自噬的调控作用

杨骐瑞; 仇兴华; 戴兴业; 刘道楠; 赵百川; 江文艺; 宋燕华; 蒲通; 程凯

doi:10.13703/j.0255-2930.20241031-k0001

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电针“内关”对心肌缺血再灌注损伤大鼠缺血期和再灌注期线粒体自噬的调控作用

机制探讨 | 更新时间：2025-05-15

- 电针“内关”对心肌缺血再灌注损伤大鼠缺血期和再灌注期线粒体自噬的调控作用
- Regulatory effect of electroacupuncture at "Neiguan" (PC6) on mitochondrial autophagy during the ischemia and reperfusion phases in rats with myocardial ischemia-reperfusion injury
- 中国针灸 2025年45卷第5期页码：646-656
- 作者机构：
  
  北京中医药大学针灸推拿学院，北京　102401
- 作者简介：
  
  杨骐瑞，北京中医药大学硕士研究生。E-mail：18810315891@163.com
  ✉程凯，教授。E-mail：kevin@chengs.cn
- 基金信息：
  
  北京中医药大学揭榜挂帅资助项目(90011431210003)
- DOI：10.13703/j.0255-2930.20241031-k0001
  中图分类号：
- 收稿：2024-10-31，
  
  网络首发：2025-02-27，
  
  纸质出版：2025-05-12
- 稿件说明：
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杨骐瑞, 仇兴华, 戴兴业, 等. 电针“内关”对心肌缺血再灌注损伤大鼠缺血期和再灌注期线粒体自噬的调控作用[J]. 中国针灸, 2025,45(5):646-656.

YANG Qirui, QIU Xinghua, DAI Xingye, et al. Regulatory effect of electroacupuncture at "Neiguan" (PC6) on mitochondrial autophagy during the ischemia and reperfusion phases in rats with myocardial ischemia-reperfusion injury[J]. Chinese Acupuncture & Moxibustion, 2025, 45(5): 646-656.
杨骐瑞, 仇兴华, 戴兴业, 等. 电针“内关”对心肌缺血再灌注损伤大鼠缺血期和再灌注期线粒体自噬的调控作用[J]. 中国针灸, 2025,45(5):646-656. DOI： 10.13703/j.0255-2930.20241031-k0001.

YANG Qirui, QIU Xinghua, DAI Xingye, et al. Regulatory effect of electroacupuncture at "Neiguan" (PC6) on mitochondrial autophagy during the ischemia and reperfusion phases in rats with myocardial ischemia-reperfusion injury[J]. Chinese Acupuncture & Moxibustion, 2025, 45(5): 646-656. DOI： 10.13703/j.0255-2930.20241031-k0001.

摘要

目的：

探讨电针“内关”对心肌缺血再灌注损伤（MIRI）大鼠不同时期（缺血期、再灌注期）线粒体自噬的调控作用，探讨电针“内关”的双向调控作用及可能机制。

方法：

将45只雄性SD大鼠按照随机数字表法分为6组，假手术组（9只）、模型A组（6只）、模型B组（9只）、电针A1组（6只）、电针B1组（6只）、电针B2组（9只）。除假手术组外，其余各组大鼠采用物理结扎加推管法复制MIRI模型，模型A组进行结扎后直接取材，模型B组进行结扎、再灌注后取材；电针A1组结扎的同时进行电针处理，之后直接取材；电针B1组结扎的同时进行电针处理，再灌注后取材；电针B2组结扎、开放冠状动脉左前降支的同时予电针处理，再灌注后取材。电针取双侧“内关”，采用疏密波，频率2 Hz/100 Hz，电流1 mA，时长为30 min。HE染色法观察大鼠心肌细胞形态，TUNEL法检测大鼠心肌细胞凋亡情况，转录组测序检测大鼠左心室差异表达基因，JC-1流式法检测大鼠心肌细胞线粒体膜电位（MMP），Western blot法检测大鼠左心室磷酸酶和张力蛋白同源物诱导激酶1（Pink1）、Parkin和p62蛋白表达，ELISA法检测大鼠血清肌酸激酶同工酶（CK-MB）、心肌肌钙蛋白I（cTn-I）含量。

结果：

与假手术组比较，模型B组大鼠心肌细胞损伤严重，排列紊乱，边界不清，肌纤维断裂，细胞水肿、松散；电针B2组大鼠心肌细胞损伤轻微，细胞结构部分不清，细胞排列较规则，可见完整心肌细胞。与假手术组比较，模型B组大鼠心肌细胞凋亡加重（

0.001）；与模型B组比较，电针B2组心肌细胞凋亡减轻（

0.001）。电针B2组、假手术组和模型B组之间的差异表达基因与细胞自噬和线粒体自噬密切相关。与假手术组比较，模型B组大鼠心肌细胞MMP降低（

0.001），左心室Pink1、Parkin、p62蛋白表达和血清CK-MB、cTn-I含量升高（

0.001）；与模型A组比较，电针A1组心肌细胞MMP和血清CK-MB、cTn-I含量降低（

0.001，

0.05），左心室Pink1蛋白表达升高（

0.01）；与模型B组比较，电针B1组和电针B2组心肌细胞MMP升高（

0.001），左心室Pink1、Parkin、p62蛋白表达及血清CK-MB、cTn-I含量降低（

0.001）；与电针A1组比较，电针B1组心肌细胞MMP升高（

0.001），左心室Pink1、Parkin、p62蛋白表达及血清CK-MB、cTn-I含量降低（

0.01）。

结论：

电针“内关”能改善大鼠MIRI，其机制可能通过Pink1/Parkin介导的线粒体自噬通路实现，在缺血期可以增强线粒体自噬来减轻心肌损伤，在再灌注期可以降低线粒体自噬来减轻再灌注损伤。

Abstract

Objective

To investigate the regulatory effect of electroacupuncture (EA) at "Neiguan" (PC6) on mitochondrial autophagy in rats with myocardial ischemia-reperfusion injury (MIRI) at different phases (ischemia and reperfusion phases)

and to explore the bidirectional regulatory effects of EA at "Neiguan" (PC6) and its potential mechanism.

Methods

Forty-five male SD rats were randomly divided into 6 groups according to the random number table method

namely

sham-operation group (

=9)

model-A group (

=6)

model-B group (

=9)

EA-A1 group (

=6)

EA-B1 group (

=6)

and EA-B2 group (

=9). Except the rats in the sham-operation group

the MIRI model was established in the other groups with the physical ligation and tube pushing method. In the model-A group

the samples

were collected directly after ligation

and in the model-B group

the samples were collected after ligation and reperfusion. In the EA-A1 group

EA was delivered while the ligation was performed

and afterwards

the samples were collected. In the EA-B1 group

while the ligation was performed

EA was operated at the same time

and after reperfusion

the samples were collected. In the EA-B2 group

during ligation and the opening of the left anterior descending branch of the coronary artery

EA was delivered

and after reperfusion

the samples were collected. EA was performed at bilateral "Neiguan" (PC6)

with a disperse-dense wave

a frequency of 2 Hz/100 Hz

a current of 1 mA

and a duration of 30 min. HE staining was employed to observe the morphology of cardiomyocytes

TUNEL was adopted to detect the apoptosis of cardiomyocytes

transcriptome sequencing was to detect the differentially expressed genes in the left ventricle

JC-1 flow cytometry was to detect the mitochondrial membrane potential (MMP) of cardiomyocytes

Western blot was to detect the protein expression of phosphatase and tensin homolog-induced kinase 1 (Pink1)

Parkin and p62 in the left ventricle of rats

and ELISA was to detect the levels of serum creatine kinase isoenzyme (CK-MB) and cardiac troponin I (cTn-I) in the rats.

Results

Compared with the sham-operation group

the cardiomyocytes of rats in the model-B group were severely damaged

with disordered arrangement

unclear boundaries

broken muscle fibers

edema and loose distribution; and the cardiomyocytes in the EA-B2 group were slightly damaged

the cell structure was partially unclear

the cells were arranged more regularly

and the intact cardiomyocytes were visible. Compared with the sham-operation group

the apoptosis of cardiomyocytes increased in the model-B group (

0.001); and when compared with the model-B group

the apoptosis alleviated in the EA-B2 group (

0.001). The differentially expressed genes among

the EA-B2 group

the sham-operation group and the model-B group were closely related to cell autophagy and mitochondrial autophagy. Compared with the sham-operation group

MMP of cardiomyocytes was reduced (

0.001)

the protein expression of Pink1

Parkin

and p62 of the left ventricle and the levels of serum CK-MB and cTn-I were elevated in the model B group (

0.001). In comparison with model-A group

the MMP of cardiomyocytes and the levels of serum CK-MB and cTn-I were reduced (

0.001

0.05)

and the protein expression of Pink1 in the left ventricle rose in the EA-A1 group (

0.01). Compared with the model-B group

MMP of cardiomyocytes increased (

0.001)

the protein expression of Pink1

Parkin

and p62 of the left ventricle

and the levels of serum CK-MB and cTn-I decreased (

0.001) in the EA-B1 group and the EA-B2 group. When compared with the EA-A1 group

MMP of cardiomyocytes increased (

0.001)

and the protein expression of Pink1

Parkin

and p62 of the left ventricle

and the levels of serum CK-MB and cTn-I decreased in the EA-B1 group (

0.01).

Conclusion

EA at "Neiguan" (PC6) can ameliorate MIRI in rats

which may be achieved through the Pink1/Parkin-mediated mitochondrial autophagy pathway. EA can alleviate myocardial injury by enhancing mitochondrial autophagy at the ischemia phase

and it can reduce reperfusion injury by weakening mitochondrial autophagy at the reperfusion phase.

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