Objective

2

To explore the acupoint compatibility patterns of acupuncture for ovarian function decline-related diseases using complex network technology

and to analyze the mechanism of core acupoints through network acupuncture methods.

Methods

2

Literature regarding acupuncture for ovarian function decline-related diseases was retrieved from CNKI

Wanfang

VIP

SinoMed

PubMed

EMbase

Cochrane Library

and Web of Science databases from their inception to April 8th

2024. Frequency analysis

association rule analysis

and complex network analysis were conducted on the selected acupoints to identify core acupoint prescriptions. Core acupoint targets were analyzed from the retrieved literature

and targets for ovarian function decline-related diseases were identified using GeneCards

Therapeutic Target

and DisGeNET databases. Protein-protein interaction (PPI) networks were constructed for intersecting targets using the STRING database. gene ontology (GO) enrichment analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis were performed using R software. A "core acupoint-target-pathway" network diagram of acupuncture for ovarian function decline-related diseases was constructed using Cytoscape software.

Results

2

A total of 133 studies were included

extracting 186 acupoint prescriptions involving 101 acupoints. The core prescription consisted of 15 acupoints

and 73 acupoint targets were identified

involving 13 core acupoints

including Guanyuan (CV4)

Sanyinjiao (SP6)

Shenshu (BL23)

Zhongwan (CV12)

Zusanli (ST36)

Zhongji (CV3)

Qihai (CV6)

Baihui (GV20)

Taichong (LR3)

Taixi (KI3)

Ciliao (BL32)

Zigong (EX-CA1)

and Guilai (ST29). There were 40 intersecting targets between the core acupoints and ovarian function decline-related diseases

with key targets including tumor protein 53

protein kinase B alpha

interleukin 1 beta

interleukin 6

interleukin 10

tumor necrosis factor

and nuclear factor kappa B subunit 1. These targets were associated with multiple signaling pathways such as T-helper 17 (Th17) cell differentiation

inflammatory bowel disease

hepatitis B

influenza A

and human immunodeficiency virus 1 (HIV-1) infection

regulating biological processes including apoptosis

inflammatory response

and immune response.

Conclusion

2

The core acupoints of acupuncture for ovarian function decline-related diseases exert therapeutic effects by acting on multiple targets and pathways

thereby delaying ovarian aging. This provides a theoretical basis for the mechanism research of acupoint prescriptions.