基于转录组测序技术探讨电针“天枢”“大肠俞”改善炎症后肠易激综合征大鼠内脏高敏感性的机制

常晓丽; 王丽君; 张丽丽; 杨宗保; 陈少宗

doi:10.13703/j.0255-2930.20250228-k0001

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基于转录组测序技术探讨电针“天枢”“大肠俞”改善炎症后肠易激综合征大鼠内脏高敏感性的机制

机制探讨 | 更新时间：2026-06-24

- 基于转录组测序技术探讨电针“天枢”“大肠俞”改善炎症后肠易激综合征大鼠内脏高敏感性的机制
- Mechanism of electroacupuncture at "Tianshu" (ST25) and "Dachangshu" (BL25) for attenuating visceral hypersensitivity in rats with post-inflammatory irritable bowel syndrome based on transcriptome sequencing technology
- 中国针灸 2026年46卷第6期页码：970-978
- 作者机构：
  
  山东中医药大学针灸研究院，济南　250355
  厦门大学医学院
- 作者简介：
  
  ✉陈少宗，教授。E-mail：ccsz1963@163.com
- 基金信息：
  
  国家重点研发计划项目(2022YFC3500602);山东省自然科学基金项目(ZR2023QH041;ZR2024QH060);山东省医药卫生科技项目(202302011716)
- DOI：10.13703/j.0255-2930.20250228-k0001
  中图分类号：
- 收稿：2025-02-28，
  
  网络首发：2025-06-04，
  
  纸质出版：2026-06-12
- 稿件说明：
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常晓丽, 王丽君, 张丽丽, 等. 基于转录组测序技术探讨电针“天枢”“大肠俞”改善炎症后肠易激综合征大鼠内脏高敏感性的机制[J]. 中国针灸, 2026,46(6):970-978.

CHANG Xiaoli, WANG Lijun, ZHANG Lili, et al. Mechanism of electroacupuncture at "Tianshu" (ST25) and "Dachangshu" (BL25) for attenuating visceral hypersensitivity in rats with post-inflammatory irritable bowel syndrome based on transcriptome sequencing technology[J]. Chinese Acupuncture & Moxibustion, 2026, 46(6): 970-978.
常晓丽, 王丽君, 张丽丽, 等. 基于转录组测序技术探讨电针“天枢”“大肠俞”改善炎症后肠易激综合征大鼠内脏高敏感性的机制[J]. 中国针灸, 2026,46(6):970-978. DOI： 10.13703/j.0255-2930.20250228-k0001.

CHANG Xiaoli, WANG Lijun, ZHANG Lili, et al. Mechanism of electroacupuncture at "Tianshu" (ST25) and "Dachangshu" (BL25) for attenuating visceral hypersensitivity in rats with post-inflammatory irritable bowel syndrome based on transcriptome sequencing technology[J]. Chinese Acupuncture & Moxibustion, 2026, 46(6): 970-978. DOI： 10.13703/j.0255-2930.20250228-k0001.

摘要

目的：

利用转录组测序技术探讨电针改善炎症后肠易激综合征（PI-IBS）大鼠内脏高敏感性的机制。

方法：

从40只SPF级雄性Wistar大鼠中随机选取12只作为对照组，剩余28只大鼠采用2

6-三硝基苯磺酸（TNBS）灌肠法制备PI-IBS模型，将造模成功的24只大鼠随机分为模型组和电针组，每组12只。电针组大鼠予电针双侧“天枢”“大肠俞”干预，采用疏密波，频率2 Hz/100 Hz，电流1 mA，每次20 min，每天1次，干预10 d。采用腹部撤退反射（AWR）实验检测大鼠内脏敏感性；腹直肌肌电图检测大鼠在不同压力结肠扩张下的曲线下面积（AUC）；HE染色观察结肠黏膜形态并计算病理评分；转录组测序技术检测结肠差异表达基因；实时荧光定量PCR法检测结肠差异基因mRNA表达。

结果：

与对照组比较，模型组大鼠在各个压力下AWR评分、AUC升高（

0.001，

0.01）；与模型组比较，电针组大鼠在各个压力下AWR评分、AUC降低（

0.001，

0.01）。模型组大鼠结肠黏膜完整性受损，伴有轻度充血、水肿和少量炎性细胞浸润；电针组大鼠结肠黏膜病变好转。各组大鼠差异表达基因中，电针组大鼠回调的基因141个。与对照组比较，模型组大鼠结肠差异表达基因主要富集在细胞因子-细胞因子受体相互作用、趋化因子信号通路、P53信号通路等；与模型组比较，电针组大鼠结肠差异表达基因主要富集在过氧化物酶体增殖物激活受体信号通路、氨基酸生物合成、P53信号通路等。电针组大鼠回调的前5位核心差异表达基因是CXC趋化因子配体10（CXCL10）、趋化因子配体20（CCL20）、CXC趋化因子受体4（CXCR4）、趋化因子配体21（CCL21）、CXC趋化因子受体5（CXCR5）。与对照组比较，模型组大鼠结肠CXCL10、CCL20 mRNA表达升高（

0.001），CXCR4 mRNA表达降低（

0.01）；与模型组比较，电针组大鼠结肠CXCL10、CCL20 mRNA表达降低（

0.01，

0.001），CXCR4 mRNA表达升高（

0.001）。

结论：

电针“天枢”“大肠俞”可改善PI-IBS大鼠的内脏高敏感性，其作用机制可能与调控CXCL10、CCL20、CXCR4基因表达有关。

Abstract

Objective

To investigate the mechanism of electroacupuncture (EA) for attenuating visceral hypersensitivity in rats with post-inflammatory irritable bowel syndrome (PI-IBS) using transcriptome sequencing technology.

Methods

Of 40 SPF grade male Wistar rats

12 rats were randomly selected as the control group. Enema of 2

6-trinitrobenzenesulfonic acid solution (TNBS) was adopted to prepare PI-IBS models among the remaining 28 rats. The successfully-modeled 24 rats were randomized into a model group and an EA group

12 rats in each one. In the EA group

EA was delivered at bilateral"Tianshu" (ST25) and "Dachangshu" (BL25)

with disperse-dense wave

at a frequency of 2 Hz/100 Hz and an electric current of 1 mA

for 20 min

once daily

and EA intervention was composed of 10 days. Visceral sensitivity was detected by the abdominal withdrawal reflex (AWR) method

and the area under the curve (AUC) of rectus abdominis electromyography(EMG) was measured under different pressures of colonic dilation. HE staining was used to observe colonic mucosal morphology and pathological score was calculated; transcriptome sequencing was applied to detect differentially expressed genes (DEGs) in the colon; and real-time fluorescent quantitative PCR was used to detect the mRNA expression of DEGs.

Results

Compared with the control group

AWR scores and AUC under each pressure increased in the model group (

0.001

0.01). Compared with the model group

EA group showed the decrease in AWR scores and AUC under each pressure (

0.001

0.01). The model group exhibited the damaged colonic mucosal integrity

accompanied by mild congestion

edema

and a small amount of inflammatory cell infiltration

while the EA group showed the alleviation in colonic mucosal lesions. Regarding DEGs

there were 141 reversed genes in the EA group. When compared with the control group

DEGs in the model group were mainly enriched in cytokine-cytokine receptor interaction

chemokine sign

aling pathway and P53 signaling pathway

etc. DEGs in the EA group were mainly enriched in peroxisome proliferator-activated receptor signaling pathway

biosynthesis of amino acid

and P53 signaling pathway in comparison with the model group. The top 5 core DEGs reversed by EA were CXC chemokine ligand 10 (CXCL10)

chemokine ligand 20 (CCL20)

CXC chemokine receptor 4(CXCR4)

chemokine ligand 21 (CCL21)

and CXC chemokine receptor 5 (CXCR5). Compared with the control group

the mRNA expression of CXCL10 and CCL20 of the colon was elevated (

0.001 )

and that of CXCR4 reduced (

0.01) in the model group. When compared with the model group

EA group showed the decrease in the mRNA expression of CXCL10 and CCL20 of the colon (

0.01

0.001)

and the increase in that of CXCR4 (

0.001).

Conclusion

Electroacupuncture at "Tianshu" (ST25) and "Dachangshu" (BL25) can attenuate visceral hypersensitivity in PI-IBS rats

probably by regulating the expression of genes such as CXCL10

CCL20

CXCR4.

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