基于lncRNA-RP4介导的Wnt/β-catenin通路探讨艾灸督脉调控自噬抗阿尔茨海默病机制研究

周冰原; 朱才丰; 陈晓宇; 李静; 高大红; 吴安琪; 陶丽; 袁心怡; 杨高尚; 陈希阳; 谢苗苗; 程乐; 王雪伟

doi:10.13703/j.0255-2930.20250309-k0001

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基于lncRNA-RP4介导的Wnt/β-catenin通路探讨艾灸督脉调控自噬抗阿尔茨海默病机制研究

机制探讨 | 更新时间：2026-06-24

- 基于lncRNA-RP4介导的Wnt/β-catenin通路探讨艾灸督脉调控自噬抗阿尔茨海默病机制研究
- Mechanism of moxibustion at the governor vessel for regulating autophagy against Alzheimer's disease via lncRNA-RP4-mediated Wnt/β-catenin pathway
- 中国针灸 2026年46卷第6期页码：929-947
- 作者机构：
  
  安徽中医药大学第一临床医学院，合肥　230038
  安徽中医药大学第二附属医院，老年病三科，合肥　230061
  安徽中医药大学第二附属医院，脑病四科，合肥　230061
  安徽中医药大学第二附属医院，医务科，合肥　230061
  安徽省中医药科学院针灸临床研究所，合肥　236231
  经脉脏腑相关安徽省重点实验室，合肥　230038
- 作者简介：
  
  周冰原，安徽中医药大学博士研究生。E-mail：1327182084@qq.com
  ✉朱才丰，主任医师。E-mail：727687720@qq.com
- 基金信息：
  
  安徽省华佗中医药研究院科技重大专项“揭榜挂帅”项目(BZKZ2402);安徽省中医药传承创新科研项目(2024CCCX004);安徽省新时代育人质量工程项目(2024xscx104);安徽中医药大学第二附属医院科研基金重大项目(2024lnbkzk01);安徽中医药大学探索性科研项目(AHUCM2024TS124);安徽省自然科学基金项目(2208085MH273);国家中医药管理局国家中医优势专科建设项目(国中医药医政函〔2024〕90号)
- DOI：10.13703/j.0255-2930.20250309-k0001
  中图分类号：
- 收稿：2025-03-09，
  
  网络首发：2025-08-19，
  
  纸质出版：2026-06-12
- 稿件说明：
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周冰原, 朱才丰, 陈晓宇, 等. 基于lncRNA-RP4介导的Wnt/β-catenin通路探讨艾灸督脉调控自噬抗阿尔茨海默病机制研究[J]. 中国针灸, 2026,46(6):929-947.

ZHOU Bingyuan, ZHU Caifeng, CHEN Xiaoyu, et al. Mechanism of moxibustion at the governor vessel for regulating autophagy against Alzheimer's disease via lncRNA-RP4-mediated Wnt/β-catenin pathway[J]. Chinese Acupuncture & Moxibustion, 2026, 46(6): 929-947.
周冰原, 朱才丰, 陈晓宇, 等. 基于lncRNA-RP4介导的Wnt/β-catenin通路探讨艾灸督脉调控自噬抗阿尔茨海默病机制研究[J]. 中国针灸, 2026,46(6):929-947. DOI： 10.13703/j.0255-2930.20250309-k0001.

ZHOU Bingyuan, ZHU Caifeng, CHEN Xiaoyu, et al. Mechanism of moxibustion at the governor vessel for regulating autophagy against Alzheimer's disease via lncRNA-RP4-mediated Wnt/β-catenin pathway[J]. Chinese Acupuncture & Moxibustion, 2026, 46(6): 929-947. DOI： 10.13703/j.0255-2930.20250309-k0001.

摘要

目的：

观察艾灸督脉介导长链非编码RNA（lncRNA-RP4）/微小RNA-939-5p（miR-939-5p）及B细胞淋巴瘤-2基因/腺病毒E1B 19 kDa相互作用蛋白3（Bnip3）调控Wnt/β-连环蛋白（β-catenin）通路对APP/PS1双转基因小鼠的影响，探究艾灸治疗阿尔茨海默病（AD）的机制。

方法：

将60只6月龄APP/PS1小鼠随机分为模型组、雷帕霉素组、艾灸+3-甲基腺嘌呤（3-MA）组、艾灸组，每组15只；15只同月龄C57BL/6J小鼠作为对照组。雷帕霉素组予腹腔注射雷帕霉素（2 mg/kg），艾灸组予隔附子饼实按灸“百会”，悬灸“风府”“大椎”各20 min，艾灸+3-MA组小鼠在艾灸组基础上按1.5 mg/kg剂量注射3-MA溶液，连续干预6次后休息1 d，共干预2周。体外培养HEK293T细胞，按照转染miR-939-5p及其空载质粒，转染lncRNA-RP4及Bnip3野生型和突变型并进行分组。体外培养HT22细胞随机分为对照组、Aβ 1-42组（模型组）；设置lncRNA-RP4过表达组、lncRNA-RP4敲减组、miR-939-5p模拟物组、miR-939-5p抑制剂组、Bnip3过表达组、Bnip3敲减组及各自对应的空载质粒组，在模型组基础上进行转染。以Morris水迷宫实验检测小鼠学习记忆能力，HE染色观察各组小鼠海马组织形态，透射电镜观察各组小鼠海马CA1区神经元结构、自噬泡及自噬溶酶体数量和结构，免疫组化法检测各组小鼠海马区Aβ 1-42蛋白表达，实时荧光定量PCR法检测各组小鼠海马区哺乳动物雷帕霉素靶蛋白（mTOR）、转录因子EB（TFEB）、泛素结合蛋白P62（P62）、Wnt3a、β-catenin、糖原合酶激酶-3β（GSK-3β）、lncRNA-RP4、miR-939-5p、Bnip3 mRNA的表达，Western blot法检测各组小鼠海马区mTOR、TFEB、P62、微管相关蛋白1轻链3B-Ⅰ（LC3B-Ⅰ）、微管相关蛋白1轻链3B-Ⅱ（LC3 B-Ⅱ）、组织蛋白酶B（CTSB）、溶酶体相关膜蛋白1（Lamp1）、V型ATP酶（V-ATPase）、Wnt3a、β-catenin、GSK-3β、Bnip3蛋白表达，双荧光素酶实验验证各组HEK293T细胞中lncRNA-RP4、miR-939-5p、Bnip3之间的靶向关系，ELISA法检测各组HT22细胞Aβ 1-42蛋白浓度，实时荧光定量PCR法检测各组HT22细胞lncRNA-RP4、miR-939-5p、Bnip3、Wnt3a、β-catenin、GSK-3β mRNA表达，Western blot法检测各组HT22细胞Bnip3、Wnt3a、β-catenin、GSK-3β蛋白表达。

结果：

与模型组比较，雷帕霉素组、艾灸组逃避潜伏期缩短（

0.05），穿越平台次数增加（

0.05）；海马神经元细胞数量较多，见少量的细胞坏死，细胞排列较有序、界线较清晰，部分神经元变形、萎缩和不规则，自噬泡增多；海马Aβ 1-42蛋白表达，mTOR、P62、GSK-3β mRNA和蛋白表达，miR-939-5p mRNA表达均降低（

0.05），TFEB、Wnt3a、β-catenin、Bnip3 mRNA和蛋白表达、LC3B-Ⅰ、LC3B-Ⅱ、CTSB、Lamp1、V-ATPase蛋白表达，lncRNA-RP4 mRNA表达均升高（

0.05）。与雷帕霉素组及艾灸组比较，艾灸+3-MA组逃避潜伏期延长（

0.05），穿越平台次数减少（

0.05）；海马神经元细胞数量小幅度减少，细胞坏死较多，细胞排列较不规则、界线较模糊，偶见少量自噬泡和较多变形神经元；海马Aβ 1-42蛋白表达，mTOR、P62、GSK-3β mRNA和蛋白表达，miR-939-5p mRNA表达均升高（

0.05）；TFEB、Wnt3a、β-catenin、Bnip3 mRNA和蛋白表达，LC3B-Ⅰ、LC3 B-Ⅱ、CTSB、Lamp1、V-ATPase蛋白表达，lncRNA-RP4 mRNA表达均降低（

0.05）。双荧光素酶实验证实lncRNA-RP4、miR-939-5p、Bnip3之间存在靶向关系。体外实验干预lncRNA-RP4后，与模型组比较，lncRNA-RP4过表达组Aβ 1-42蛋白浓度、miR-939-5p mRNA表达、GSK-3β mRNA和蛋白表达均降低（

0.05），lncRNA-RP4 mRNA表达及Bnip3、Wnt3a、β-catenin mRNA和蛋白表达均升高（

0.05）；lncRNA-RP4敲减组Aβ 1-42蛋白浓度、miR-939-5p mRNA表达、GSK-3β mRNA和蛋白表达均升高（

0.05），lncRNA-RP4 mRNA表达及Bnip3、Wnt3a、β-catenin mRNA和蛋白表达均降低（

0.05）。与lncRNA-RP4过表达组比较，lncRNA-RP4敲减组Aβ 1-42蛋白浓度、miR-939-5p mRNA表达、GSK-3β mRNA和蛋白表达均升高（

0.05），lncRNA-RP4 mRNA表达及Bnip3、Wnt3a、β-catenin mRNA和蛋白表达均降低（

0.05）。干预miR-939-5p后，与模型组比较，miR-939-5p模拟物组Aβ 1-42蛋白浓度、miR-939-5p mRNA表达、GSK-3β mRNA和蛋白表达均升高（

0.05），lncRNA-RP4 mRNA表达及Bnip3、Wnt3a、β-catenin mRNA和蛋白表达均降低（

0.05）；miR-939-5p抑制剂组Aβ 1-42蛋白浓度、miR-939-5p mRNA表达、GSK-3β mRNA和蛋白表达均降低（

0.05），lncRNA-RP4 mRNA表达及Bnip3、Wnt3a、β-catenin mRNA和蛋白表达均升高（

0.05）。与miR-939-5p模拟物组比较，miR-939-5p抑制剂组Aβ 1-42蛋白浓度、miR-939-5p mRNA表达、GSK-3β mRNA和蛋白表达均降低（

0.05），lncRNA-RP4 mRNA表达及Bnip3、Wnt3a、β-catenin mRNA和蛋白表达均升高（

0.05）。干预Bnip3后，与模型组比较，Bnip3过表达组Aβ 1-42蛋白浓度、miR-939-5p mRNA表达、GSK-3β mRNA和蛋白表达均降低（

0.05），lncRNA-RP4 mRNA表达及Bnip3、Wnt3a、β-catenin mRNA和蛋白表达均升高（

0.05）；Bnip3敲减组Aβ 1-42蛋白浓度、miR-939-5p mRNA表达及GSK-3β mRNA和蛋白表达均升高（

0.05），lncRNA-RP4 mRNA表达及Bnip3、Wnt3a、β-catenin mRNA和蛋白表达均降低（

0.05）。与Bnip3过表达组比较，Bnip3敲减组Aβ 1-42蛋白浓度、miR-939-5p mRNA表达、GSK-3β mRNA和蛋白表达均升高（

0.05），lncRNA-RP4 mRNA表达及Bnip3、Wnt3a、β-catenin mRNA和蛋白表达均降低（

0.05）。

结论：

艾灸督脉可通过调节lncRNA-RP4/miR-939-5p/Bnip3轴激活Wnt/β-catenin通路，改善自噬溶酶体活性，促进细胞自噬，加速Aβ 1-42清除，改善AD认知功能障碍。

Abstract

Objective

To observe the effect of moxibustion at the governor vessel on lncRNA-RP4/miR-939-5p and Bnip3 in APP/PS1 double transgenic mice mediated by Wnt/β-catenin pathway

and to explore the mechanism of moxibustion in the treatment of Alzheimer's disease (AD).

Methods

Sixty 6-month-old APP/PS1 mice were randomly divided into a model group

a rapamycin group

a moxibustion+ 3-methyladenine (3-MA) group and a moxibustion group

with 15 mice in each group. Fifteen C57BL/6J mice of the same age were used as the control group. The rapamycin group was given intraperitoneal injection of rapamycin (2 mg/kg). The moxibustion group was given moxibustion at "Baihui" (GV20)

suspended moxibustion at "Fengfu" (GV16) and "Dazhui" (GV14) for 20 min. The moxibustion+3-MA group was injected with 1.5 mg/kg 3-MA on the basis of the moxibustion group. After 6 consecutive treatments

rest for 1 d

and lasted 2 weeks.HEK293T cells were cultured in vitro and transfected with miR-939-5p and its empty plasmid

and transfected with lncRNA-RP4 and Bnip3 wild-type and mutant. HT22 cells cultured in vitro were randomly divided into a control group and a model (Aβ 1-42) group. The lncRNA-RP4 overexpression group

the lncRNA-RP4 knockdown group

the miR-939-5p mimic group

the miR-939-5p inhibitor group

the Bnip3 overexpression group

the Bnip3 knockdown group and the corresponding empty plasmid group were set up

and transfection was performed on the basis of the model group. Morris water maze test was used to detect the learning and memory ability of mice. HE staining was used to observe the morphology of hippocampus in each group. The structure of nerve cells

the number and structure of autophagic vacuoles and autophagic lysosomes in hippocampal CA1 region of mice in each group were observed by transmission electron microscopy. The expression of Aβ 1-42 protein in hippocampus was detected by immunohistochemistry. The expression of mTOR

TFEB

P62

Wnt3 a

β-catenin

GSK-3β

lncRNA-RP4

miR-939-5 p and Bnip3 mRNA in hippocampus of mice in each group was detected by real-time fluorescence quantitative PCR. Western blot was used to detect the expression of mTOR

TFEB

P62

LC3 B-Ⅰ

LC3 B-Ⅱ

CTSB

Lamp1

V-ATPase

Wnt3a

β-catenin

GSK-3β and Bnip3 protein in hippocampus of mice in each group.Dual luciferase assay was used to verify the targeting relationships among lncRNA-RP4

miRNA-939-5p and Bnip3 in HEK293T cells. The concentration of Aβ 1-42 in HT22 cells of each group was detected by ELISA. The expression of lncRNA-RP4

miR-939-5p

Bnip3

Wnt3a

β-catenin and GSK-3β mRNA in HT22 cells of each group was detected by real-time fluorescence quantitative PCR. The expression of Bnip3

Wnt3a

β- catenin and GSK-3β protein in HT22 cells of each group was detected by Western blot.

Results

Compared with the model group

the escape latency of the rapamycin group and the moxibustion group was shortened (

0.05)

and the number of crossing the platform was increased (

0.05).The number of hippoc

ampal neurons was large

and a small amount of cell necrosis was observed. The cells were arranged in an orderly manner with clear boundaries. Some neurons were deformed

atrophied and irregular

and autophagic vacuoles increased. The expression of A β 1-42 protein

mTOR

P62

GSK-3β mRNA and protein

and miR-939-5p mRNA in hippocampus was decreased (

0.05)

while the expression of TFEB

Wnt3a

β-catenin

Bnip3 mRNA and protein

LC3B-Ⅰ

LC3B-Ⅱ

CTSB

Lamp1

V-ATPase protein

and lncRNA-RP4 mRNA was increased (

0.05). Compared with the rapamycin and moxibustion groups

the escape latency of the moxibustion+3-MA group was prolonged (

0.05)

and the number of crossing the platform was decreased (

0.05). The number of hippocampal neurons decreased slightly

the cell necrosis was more

the cell arrangement was irregular

the boundary was blurred

and a small amount of autophagic vacuoles and more deformed neurons were occasionally seen. The expression of A β 1-42 protein

mTOR

P62

GSK-3β mRNA and protein

and miR-939-5p mRNA in hippocampus increased (

0.05)

while the expression of TFEB

Wnt3a

β-catenin

Bnip3 mRNA and protein

LC3B-Ⅰ

LC3B-Ⅱ

CTSB

Lamp1

V-ATPase protein

and lncRNA-RP4 mRNA decreased (

0.05). Dual luciferase assay confirmed that there was a targeting relationship among lncRNA-RP4

miR-939-5p and Bnip3.After the intervention of lncRNA-RP4 in vitro

compared with the model group

the expression of Aβ 1-42 protein

miR-939-5p mRNA

GSK-3 β mRNA and protein in the lncRNA-RP4 overexpression group was decreased (

0.05)

and the expression of lncRNA-RP4 mRNA

Bnip3

Wnt3a

β-catenin mRNA and protein was increased (

0.05). The expression of Aβ 1-42 protein

miR-939-5p mRNA

GSK-3 β mRNA and protein in lncRNA-RP4 knockdown group was increased (

0.05)

while the expression of lncRNA-RP4 mRNA

Bnip3

Wnt3a and β-catenin mR

NA and protein was decreased (

0.05). Compared with the lncRNA-RP4 overexpression group

the expression of Aβ 1-42 protein

miR-939-5p mRNA

GSK-3 β mRNA and protein in the lncRNA-RP4 knockdown group was increased (

0.05)

and the expression of lncRNA-RP4 mRNA

Bnip3

Wnt3a

β-catenin mRNA and protein was decreased (

0.05). After intervention with miR-939-5p

compared with the model group

the expression of Aβ 1-42 protein

miR-939-5p mRNA

GSK-3 β mRNA and protein in the miR-939-5p mimic group was increased (

0.05)

and the expression of lncRNA-RP4 mRNA

Bnip3

Wnt3a

β-catenin mRNA and protein was decreased (

0.05). The expression of A β 1-42 protein

miR-939-5p mRNA

GSK-3 β mRNA and protein in miR-939-5p inhibitor group was decreased (

0.05)

while the expression of lncRNA-RP4 mRNA

Bnip3

Wnt3a and β-catenin mRNA and protein was increased (

0.05). Compared with the miR-939-5p mimic group

the expression of Aβ 1-42 protein

miR-939-5p mRNA

GSK-3β mRNA and protein in the miR-939-5p inhibitor group was decreased (

0.05)

and the expression of lncRNA-RP4 mRNA

Bnip3

Wnt3a

β-catenin mRNA and protein was increased (

0.05). After Bnip3 intervention

compared with the model group

the expression of Aβ 1-42 protein

miR-939-5p mRNA

GSK-3 β mRNA and protein in the Bnip3 overexpression group was decreased (

0.05)

while the expression of lncRNA-RP4 mRNA

Bnip3

Wnt3a and β-catenin mRNA and protein was increased (

0.05). The expression of A β 1-42 protein

miR-939-5p mRNA

GSK-3 β mRNA and protein in the Bnip3 knockdown group was increased (

0.05)

while the expression of lncRNA-RP4 mRNA

Bnip3

Wnt3a and β-catenin mRNA and protein was decreased (

0.05). Compared with the Bnip3 overexpression group

the expression of Aβ 1-42 protein

miR-939-5p mRNA

SK-3β mRNA and protein in Bnip3 knockdown group was increased (

0.05)

and the expression of lncRNA-RP4 mRNA

Bnip3

Wnt3a

β-catenin mRNA and protein was decreased (

0.05).

Conclusion

Moxibustion at the governor vessel ameliorates AD cognitive deficits by activating the lncRNA-RP4/miR-939-5p/Bnip3 axis

enhancing Wnt/β-catenin pathway

restoring autophagosome-lysosome activity

promoting autophagy

accelerating A β 1-42 clearance

and improve cognitive dysfunction of AD. This study elucidates a novel epigenetic mechanism underlying moxibustion's therapeutic efficacy in AD.

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