This paper presents the design of a mouse multi-site fixation device for acupuncture experiment. The fixation device comprises a multi-modal body exposure system

a neck dynamic adjustment system

and a head fixation system

designed for easy operation and remained fixation. It is not only capable for acupuncture experiment of mouse under non-anesthetized conditions and improving the accuracy of experimental data

but also ensures the full explosion of points on the mouse's torso

four limbs

abdomen

head and face

facilitating acupuncture delivered simultaneously at multiple points. The device is made by 3D printing

which has many advantages as personalization

lightweight

low cost and quick production.