Objective

2

To investigate the effect and potential mechanism of electroacupuncture (EA) at the acupoints of the hand-

jueyin

pericardium meridian on oxidative stress-related proteins in ischemic stroke rats.

Methods

2

Of 108 SD male rats

27 rats were randomly assigned to a sham-operation group

and the rest 81 rats were in a modeling group and prepared the middle cerebral artery occlusion (MCAO) models by inserting a suture into the carotid artery. The 81 successfully modeled rats were randomized into a model group

a pericardium meridian group and a non-meridian point group

27 rats in each one; and each group was subdivided into a 1-day group (12 rats) and a 7-day group (15 rats) according to intervention duration. In the pericardium meridian group

EA was delivered at "Tianquan" (PC2)

"Quze" (PC3)

"Neiguan" (PC6)

and "Daling" (PC7) on the right side; and the electric stimulation was attached to two pairs of points "Tianquan" (PC2) and "Quze" (PC3)

and "Neiguan" (PC6) and "Daling" (PC7)

respectively

with continuous wave

at the frequency of 1 Hz to 20 Hz and a current intensity of 4 mA to 6 mA

30 min each time. In the non-meridian point group

the sites 1.5 mm lateral to "Tianquan" (PC2)

"Quze" (PC3)

"Neiguan" (PC6) and "Daling" (PC7) on the right side were located and stimulated with the same procedure as the pericardium meridian group. The intervention in each subgroup was conducted for 1 day and 7 days respectively. The Zea Longa score and Bederson score were used to evaluate the neurological deficits in rats. TTC staining was adopted to determine the infarct volume

HE staining and Nissl staining were employed to observe the morphology of brain tissue

and transmission electron microscopy was utilized to examine the ultrastructure of mitochondria in neurons on the ischemic side. Using ELISA

the levels of nuclear factor E2-related factor 2 (Nrf2)

heme oxygenase 1 (HO-1)

and peroxisome proliferator-γ coactivator-1α (PGC-1α) in serum were detected. Using Western blot and real-time fluorescent quantitative PCR

the protein and mRNA expression of Nrf2

HO-1

and PGC-1α in brain tissue on the ischemic side was detected.

Results

2

The neurological deficits and pathological changes were not found in the sham-operation group. In 1 and 7 days of intervention

in the model group

the neuronal density in the infarcted area was reduced and neuronal edema was obvious; and when compared with the sham-operation group

the neurological deficit scores were higher (

P

<

0.001)

the volume of cerebral infarction increased (

P

<

0.001) and the number of cortical neurons decreased (

P

<

0.001). In 7 days of intervention

compared with the sham-operation group

the levels of serum Nrf2

HO-1

and PGC-1α were reduced (

P

<

0.01)

and the protein and mRNA expression of Nrf2

HO-1

and PGC-1α in brain tissue on the ischemic side decreased (

P

<

0.001) in the model group; neuronal cell edema was alleviated

necrotic cells were declined

and mitochondrial morphology was improved in the pericardium meridian group

and the number of neurons in the cerebral cortex was higher than that in the model group (

P

<

0.01). When compared with the model group and non-meridian point group

the levels of serum Nrf2

HO-1 and PGC-1α were elevated (

P

<

0.001)

and the protein and mRNA expression of Nrf2

HO-1 and PGC-1α in brain tissue on the ischemic side increased in the pericardium meridian group (

P

<

0.001).

Conclusion

2

Electroacupuncture at the acupoints of hand-

jueyin

pericardium meridian alleviates neurological deficits and promotes neurological functional recovery in MCAO rats

which may be mediated by upregulating the expression of Nrf2

HO-1 and PGC-1α

thereby mitigating oxidative stress injury.