It attempts to determine the theoretical connotation and clinical application of the twelve-meridian based syndrome/pattern differentiation of TCM through the systematic analysis and elaboration

so as to promote the completion of meridian differentiation system. The exploration is conducted on the main body of traditional meridian-syndrome differentiation

meaning the meridian differentiation in terms of location of illness and that in terms of symptoms. The existing problems and causes are analyzed

and the specific methods of meridian differentiation put forward in line with the characteristics of meridian distribution and symptoms. In reference with

Huangdi Neijing

(

Yellow Emperors

’

Canon of Medicine

) and other ancient literature

the theoretical evidences of meridian differentiation are deeply analyzed in view of physiological/pathological characteristics that has been neglected in the past

such as

qi

and blood of meridians

opening

closing and pivoting

and time. Additionally

the category issues related to twelve-meridian differentiation and their relationship with six-meridian differentiation are expounded. The summary on the multiple connotations of twelve-meridian differentiation is of great significance on re-understanding meridians

perfecting meridian-collateral differentiation system and improving the accuracy on meridian-based treatment. Besides

the reconstruction of meridian differentiation and its framework is considered profoundly.