The "intelligence three-needling" therapy

developed by Professor

JIN Rui

the eminent acupuncture- moxibustion master in the south of Five Ridges

is effective on cognitive dysfunction in practice. The paper reviews the animal experiment researches of Alzheimer's disease (AD) treated with this therapy

aiming to explain its core mechanism and effect characteristics for AD. The results showed that the "intelligence three-needling" therapy exerts its effect through multiple targets and diverse pathways. It improves cholinergic system function

enhances glucose metabolism in brain regions

reduces oxidative stress damage

suppresses neuroinflammation

regulates the Wnt/β-catenin signaling pathway

promotes autophagy-lysosomal clearance of pathological proteins

activates the transmembrane receptor protein (Notch) pathway to strengthen synaptic plasticity

demonstrates neuroprotective and anti-apoptotic effects

and modulates gut microbiota. In experiments

this therapy demonstrated specific effect in brain regions of 5xFAD mouse models. Compared with the single ap

plication at "Benshen" (GB13) or "Shenting" (GV24)

the acupoint combination in this therapy displayed the synergistic advantages

regulating more comprehensively adenosine monophosphate activated protein kinase (AMPK)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) kinase network. This review provides the theoretical basis for optimizing the protocol of acupuncture and moxibustion for AD

and is conducive to promoting the deep integration of traditional acupuncture-moxibustion therapy with modern neuroscience.